Adjuvant activity of cordycepin, a natural derivative of adenosine from Cordyceps militaris, on an inactivated rabies vaccine in an animal model
Vaccination is the most feasible way of preventing rabies, an ancient zoonosis that remains a major public health concern globally. However, administration of inactivated rabies vaccination without adjuvants is always inefficient and necessitates four to five injections. In the current study, we exp...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024006431 |
| _version_ | 1797328604873359360 |
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| author | Xin Chen Boyu Liao Tianci Ren Zhipeng Liao Zijie Huang Yujuan Lin Shouhao Zhong Jiaying Li Shun Wen Yingyan Li Xiaohan Lin Xingchen Du Yuhui Yang Jiubiao Guo Xiaohui Zhu Haishu Lin Rui Liu Jingbo Wang |
| author_facet | Xin Chen Boyu Liao Tianci Ren Zhipeng Liao Zijie Huang Yujuan Lin Shouhao Zhong Jiaying Li Shun Wen Yingyan Li Xiaohan Lin Xingchen Du Yuhui Yang Jiubiao Guo Xiaohui Zhu Haishu Lin Rui Liu Jingbo Wang |
| author_sort | Xin Chen |
| collection | DOAJ |
| description | Vaccination is the most feasible way of preventing rabies, an ancient zoonosis that remains a major public health concern globally. However, administration of inactivated rabies vaccination without adjuvants is always inefficient and necessitates four to five injections. In the current study, we explored the adjuvant characteristics of cordycepin, a major bioactive component of Cordyceps militaris, to boost immune responses against a commercially available rabies vaccine. We found that cordycepin could stimulate stronger phenotypic and functional maturation of dendritic cells (DCs). For animal experiments, mice were immunized 3 times with rabies vaccine in the presence or absence of cordycepin at 1-week interval. Analysis of T cell differentiation and serum antibody isotypes showed that humoral immunity was dominant with a Th2 biased immune response. These results were also supported by the raised ratio of follicular helper T cells (TFH) and germinal center B cells (GCB). Thus, titer of rabies virus neutralizing antibody (RVNAb) and rabies virus-specific memory B cells were both raised as a result. Furthermore, administration of cordycepin did not cause pathological phenomena or body weight loss. The findings indicate that cordycepin could be used as a promising adjuvant for rabies vaccines to get a higher range of protection without any side effects. |
| first_indexed | 2024-03-08T06:55:03Z |
| format | Article |
| id | doaj.art-808711d998a64dc3b81f5c1814d1a488 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T06:55:03Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-808711d998a64dc3b81f5c1814d1a4882024-02-03T06:38:01ZengElsevierHeliyon2405-84402024-01-01102e24612Adjuvant activity of cordycepin, a natural derivative of adenosine from Cordyceps militaris, on an inactivated rabies vaccine in an animal modelXin Chen0Boyu Liao1Tianci Ren2Zhipeng Liao3Zijie Huang4Yujuan Lin5Shouhao Zhong6Jiaying Li7Shun Wen8Yingyan Li9Xiaohan Lin10Xingchen Du11Yuhui Yang12Jiubiao Guo13Xiaohui Zhu14Haishu Lin15Rui Liu16Jingbo Wang17College of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, China; School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, China; School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, China; School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, China; School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, 518118, ChinaLaboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200438, China; Corresponding author.College of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, China; School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, 518118, China; Corresponding author. College of Pharmacy, Shenzhen Technology University, Shenzhen, 518118, China.Vaccination is the most feasible way of preventing rabies, an ancient zoonosis that remains a major public health concern globally. However, administration of inactivated rabies vaccination without adjuvants is always inefficient and necessitates four to five injections. In the current study, we explored the adjuvant characteristics of cordycepin, a major bioactive component of Cordyceps militaris, to boost immune responses against a commercially available rabies vaccine. We found that cordycepin could stimulate stronger phenotypic and functional maturation of dendritic cells (DCs). For animal experiments, mice were immunized 3 times with rabies vaccine in the presence or absence of cordycepin at 1-week interval. Analysis of T cell differentiation and serum antibody isotypes showed that humoral immunity was dominant with a Th2 biased immune response. These results were also supported by the raised ratio of follicular helper T cells (TFH) and germinal center B cells (GCB). Thus, titer of rabies virus neutralizing antibody (RVNAb) and rabies virus-specific memory B cells were both raised as a result. Furthermore, administration of cordycepin did not cause pathological phenomena or body weight loss. The findings indicate that cordycepin could be used as a promising adjuvant for rabies vaccines to get a higher range of protection without any side effects.http://www.sciencedirect.com/science/article/pii/S2405844024006431Rabies vaccineCordycepinAdjuvant |
| spellingShingle | Xin Chen Boyu Liao Tianci Ren Zhipeng Liao Zijie Huang Yujuan Lin Shouhao Zhong Jiaying Li Shun Wen Yingyan Li Xiaohan Lin Xingchen Du Yuhui Yang Jiubiao Guo Xiaohui Zhu Haishu Lin Rui Liu Jingbo Wang Adjuvant activity of cordycepin, a natural derivative of adenosine from Cordyceps militaris, on an inactivated rabies vaccine in an animal model Heliyon Rabies vaccine Cordycepin Adjuvant |
| title | Adjuvant activity of cordycepin, a natural derivative of adenosine from Cordyceps militaris, on an inactivated rabies vaccine in an animal model |
| title_full | Adjuvant activity of cordycepin, a natural derivative of adenosine from Cordyceps militaris, on an inactivated rabies vaccine in an animal model |
| title_fullStr | Adjuvant activity of cordycepin, a natural derivative of adenosine from Cordyceps militaris, on an inactivated rabies vaccine in an animal model |
| title_full_unstemmed | Adjuvant activity of cordycepin, a natural derivative of adenosine from Cordyceps militaris, on an inactivated rabies vaccine in an animal model |
| title_short | Adjuvant activity of cordycepin, a natural derivative of adenosine from Cordyceps militaris, on an inactivated rabies vaccine in an animal model |
| title_sort | adjuvant activity of cordycepin a natural derivative of adenosine from cordyceps militaris on an inactivated rabies vaccine in an animal model |
| topic | Rabies vaccine Cordycepin Adjuvant |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024006431 |
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