Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH
The design and synthesis of fluorescent probes for monitoring pH values inside living cells have attracted great attention, due to the important role pH plays in many biological processes. In this study, the optical properties of two different two-photon fluorescent probes for pH are studied. The ra...
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| Format: | Article |
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MDPI AG
2018-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/18/12/4407 |
| _version_ | 1828395616680542208 |
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| author | Yujin Zhang Wei Hu |
| author_facet | Yujin Zhang Wei Hu |
| author_sort | Yujin Zhang |
| collection | DOAJ |
| description | The design and synthesis of fluorescent probes for monitoring pH values inside living cells have attracted great attention, due to the important role pH plays in many biological processes. In this study, the optical properties of two different two-photon fluorescent probes for pH are studied. The ratiometric sensing of the probes are theoretically illustrated. Meanwhile, the recognitional mechanisms of the probes are investigated, which shows the energy transfer process when react with H<sup>+</sup>. Specially, the calculated results demonstrate that Probe1 possesses a higher energy transfer efficiency and a larger two-photon absorption cross-section than Probe2, indicating it to be a preferable pH fluorescent probe. Therefore, the influence of connection between the donor and the acceptor on the sensing performances of the probe is demonstrated. Our results help to understand the experimental observations and provide a theoretical basis to synthesize efficient two-photon fluorescent probes for monitoring pH changes. |
| first_indexed | 2024-12-10T08:16:10Z |
| format | Article |
| id | doaj.art-808ba6cfb399488d85d62e0eb7e9734b |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T08:16:10Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-808ba6cfb399488d85d62e0eb7e9734b2022-12-22T01:56:28ZengMDPI AGSensors1424-82202018-12-011812440710.3390/s18124407s18124407Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pHYujin Zhang0Wei Hu1School of Electronic and Information Engineering (Department of Physics), Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaSchool of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaThe design and synthesis of fluorescent probes for monitoring pH values inside living cells have attracted great attention, due to the important role pH plays in many biological processes. In this study, the optical properties of two different two-photon fluorescent probes for pH are studied. The ratiometric sensing of the probes are theoretically illustrated. Meanwhile, the recognitional mechanisms of the probes are investigated, which shows the energy transfer process when react with H<sup>+</sup>. Specially, the calculated results demonstrate that Probe1 possesses a higher energy transfer efficiency and a larger two-photon absorption cross-section than Probe2, indicating it to be a preferable pH fluorescent probe. Therefore, the influence of connection between the donor and the acceptor on the sensing performances of the probe is demonstrated. Our results help to understand the experimental observations and provide a theoretical basis to synthesize efficient two-photon fluorescent probes for monitoring pH changes.https://www.mdpi.com/1424-8220/18/12/4407fluorescent probetwo-photon absorptionpH sensortime-dependent density functional theory |
| spellingShingle | Yujin Zhang Wei Hu Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH Sensors fluorescent probe two-photon absorption pH sensor time-dependent density functional theory |
| title | Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH |
| title_full | Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH |
| title_fullStr | Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH |
| title_full_unstemmed | Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH |
| title_short | Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH |
| title_sort | sensing performance and efficiency of two energy transfer based two photon fluorescent probes for ph |
| topic | fluorescent probe two-photon absorption pH sensor time-dependent density functional theory |
| url | https://www.mdpi.com/1424-8220/18/12/4407 |
| work_keys_str_mv | AT yujinzhang sensingperformanceandefficiencyoftwoenergytransferbasedtwophotonfluorescentprobesforph AT weihu sensingperformanceandefficiencyoftwoenergytransferbasedtwophotonfluorescentprobesforph |