Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation
Summary: Chronic pain remains challenging to treat, despite numerous reports of its pathogenesis, including neuronal plasticity in the spinal dorsal horn (SDH). We hypothesized that understanding plasticity only at a specific time point after peripheral nerve injury (PNI) is insufficient to solve ch...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222018272 |
| _version_ | 1811304778855088128 |
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| author | Miyuki Kurabe Mika Sasaki Kenta Furutani Hidemasa Furue Yoshinori Kamiya Hiroshi Baba |
| author_facet | Miyuki Kurabe Mika Sasaki Kenta Furutani Hidemasa Furue Yoshinori Kamiya Hiroshi Baba |
| author_sort | Miyuki Kurabe |
| collection | DOAJ |
| description | Summary: Chronic pain remains challenging to treat, despite numerous reports of its pathogenesis, including neuronal plasticity in the spinal dorsal horn (SDH). We hypothesized that understanding plasticity only at a specific time point after peripheral nerve injury (PNI) is insufficient to solve chronic pain. Here, we analyzed the temporal changes in synaptic transmission and astrocyte-neuron interactions in SDH after PNI. We found that synaptic transmission in the SDH after PNI changed in a time-dependent manner, which was accompanied by astrocyte proliferation and loss of inhibitory and excitatory neurons. Furthermore, neuronal loss was accompanied by necroptosis. Short-term inhibition of astrocytes after PNI suppressed these physiological and morphological changes and long-term pain-related behaviors. These results are the first to demonstrate that the inhibition of astrocyte proliferation after PNI contributes to the long-term regulation of plasticity and of necroptosis development in the SDH. |
| first_indexed | 2024-04-13T08:13:41Z |
| format | Article |
| id | doaj.art-808bf8f90042401e89539af0ded7b502 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-13T08:13:41Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-808bf8f90042401e89539af0ded7b5022022-12-22T02:54:53ZengElsevieriScience2589-00422022-12-012512105555Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activationMiyuki Kurabe0Mika Sasaki1Kenta Furutani2Hidemasa Furue3Yoshinori Kamiya4Hiroshi Baba5Division of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences, Chuo-Ku, Niigata City 951-8510, Japan; Corresponding authorDivision of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences, Chuo-Ku, Niigata City 951-8510, JapanDivision of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences, Chuo-Ku, Niigata City 951-8510, JapanDepartment of Neurophysiology, Hyogo College of Medicine, Nishinomiya 663-8501, JapanDivision of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences, Chuo-Ku, Niigata City 951-8510, JapanDivision of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences, Chuo-Ku, Niigata City 951-8510, JapanSummary: Chronic pain remains challenging to treat, despite numerous reports of its pathogenesis, including neuronal plasticity in the spinal dorsal horn (SDH). We hypothesized that understanding plasticity only at a specific time point after peripheral nerve injury (PNI) is insufficient to solve chronic pain. Here, we analyzed the temporal changes in synaptic transmission and astrocyte-neuron interactions in SDH after PNI. We found that synaptic transmission in the SDH after PNI changed in a time-dependent manner, which was accompanied by astrocyte proliferation and loss of inhibitory and excitatory neurons. Furthermore, neuronal loss was accompanied by necroptosis. Short-term inhibition of astrocytes after PNI suppressed these physiological and morphological changes and long-term pain-related behaviors. These results are the first to demonstrate that the inhibition of astrocyte proliferation after PNI contributes to the long-term regulation of plasticity and of necroptosis development in the SDH.http://www.sciencedirect.com/science/article/pii/S2589004222018272Molecular biologyNeuroscienceCell biology |
| spellingShingle | Miyuki Kurabe Mika Sasaki Kenta Furutani Hidemasa Furue Yoshinori Kamiya Hiroshi Baba Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation iScience Molecular biology Neuroscience Cell biology |
| title | Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation |
| title_full | Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation |
| title_fullStr | Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation |
| title_full_unstemmed | Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation |
| title_short | Structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation |
| title_sort | structural and functional properties of spinal dorsal horn neurons after peripheral nerve injury change overtime via astrocyte activation |
| topic | Molecular biology Neuroscience Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222018272 |
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