Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1
<p>Abstract</p> <p>Background</p> <p>HBO1 (histone acetyltransferase binding to ORC1) is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. However, the biological role and underlying mechanism of HBO1 in breast cancer remains large...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-11-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Online Access: | http://www.jeccr.com/content/29/1/140 |
| _version_ | 1818758904118509568 |
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| author | Sun Lin-lin Zhou Lei Wu Wei-bin Liu Ye-heng Yang Jun-wu Hong Yi Wu Yi-hong Liu Hai-ou Wang Wen-zhong Xu Jie-jie Yun Xiao-jing Gu Jian-xin |
| author_facet | Sun Lin-lin Zhou Lei Wu Wei-bin Liu Ye-heng Yang Jun-wu Hong Yi Wu Yi-hong Liu Hai-ou Wang Wen-zhong Xu Jie-jie Yun Xiao-jing Gu Jian-xin |
| author_sort | Sun Lin-lin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>HBO1 (histone acetyltransferase binding to ORC1) is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. However, the biological role and underlying mechanism of HBO1 in breast cancer remains largely unknown. In the current study, we aimed to investigate the role of HBO1 in breast cancer and uncover the underlying molecular mechanism.</p> <p>Methods</p> <p>Immunohistochemistry was applied to detect HBO1 protein expression in breast cancer specimens (n = 112). The expression of protein level was scored by integral optical density (IOD) for further statistical analyses using SPSS. Real-time PCR was used to simultaneously measure mRNA levels of HBO1. The HBO1 protein expression in breast cancer cells was confirmed by western blot.</p> <p>Results</p> <p>HBO1 was highly expressed in breast cancer tissues and significantly correlated with estrogen receptor α (ERα) (p < 0.001) and progestational hormone (PR) (p = 0.002). HBO1 protein level also correlated positively with histology grade in ERα positive tumors (p = 0.016) rather than ERα negative tumors. 17β-estradiol (E2) could upregulate HBO1 gene expression which was significantly inhibited by ICI 182,780 or ERα RNAi. E2-increased HBO1 protein expression was significantly suppressed by treatment with inhibitor of MEK1/2 (U0126) in T47 D and MCF-7 cells.</p> <p>Conclusions</p> <p>HBO1 was an important downstream molecule of ERα, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.</p> |
| first_indexed | 2024-12-18T06:34:14Z |
| format | Article |
| id | doaj.art-808c15b244f849958eb95ab8f63339b7 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-18T06:34:14Z |
| publishDate | 2010-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-808c15b244f849958eb95ab8f63339b72022-12-21T21:17:50ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662010-11-0129114010.1186/1756-9966-29-140Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1Sun Lin-linZhou LeiWu Wei-binLiu Ye-hengYang Jun-wuHong YiWu Yi-hongLiu Hai-ouWang Wen-zhongXu Jie-jieYun Xiao-jingGu Jian-xin<p>Abstract</p> <p>Background</p> <p>HBO1 (histone acetyltransferase binding to ORC1) is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. However, the biological role and underlying mechanism of HBO1 in breast cancer remains largely unknown. In the current study, we aimed to investigate the role of HBO1 in breast cancer and uncover the underlying molecular mechanism.</p> <p>Methods</p> <p>Immunohistochemistry was applied to detect HBO1 protein expression in breast cancer specimens (n = 112). The expression of protein level was scored by integral optical density (IOD) for further statistical analyses using SPSS. Real-time PCR was used to simultaneously measure mRNA levels of HBO1. The HBO1 protein expression in breast cancer cells was confirmed by western blot.</p> <p>Results</p> <p>HBO1 was highly expressed in breast cancer tissues and significantly correlated with estrogen receptor α (ERα) (p < 0.001) and progestational hormone (PR) (p = 0.002). HBO1 protein level also correlated positively with histology grade in ERα positive tumors (p = 0.016) rather than ERα negative tumors. 17β-estradiol (E2) could upregulate HBO1 gene expression which was significantly inhibited by ICI 182,780 or ERα RNAi. E2-increased HBO1 protein expression was significantly suppressed by treatment with inhibitor of MEK1/2 (U0126) in T47 D and MCF-7 cells.</p> <p>Conclusions</p> <p>HBO1 was an important downstream molecule of ERα, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.</p>http://www.jeccr.com/content/29/1/140 |
| spellingShingle | Sun Lin-lin Zhou Lei Wu Wei-bin Liu Ye-heng Yang Jun-wu Hong Yi Wu Yi-hong Liu Hai-ou Wang Wen-zhong Xu Jie-jie Yun Xiao-jing Gu Jian-xin Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1 Journal of Experimental & Clinical Cancer Research |
| title | Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1 |
| title_full | Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1 |
| title_fullStr | Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1 |
| title_full_unstemmed | Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1 |
| title_short | Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1 |
| title_sort | estrogen receptor α erα mediates 17β estradiol e2 activated expression of hbo1 |
| url | http://www.jeccr.com/content/29/1/140 |
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