Decreased FMR1 mRNA levels found in men with substance use disorders
FMR1 gene (fragile X mental retardation 1) represents a genetic and epigenetic factor in a number of human diseases. Though the role of FMR1 gene in substance use disorders (SUDs) is not well studied, a number of investigations indicate that SUDs and FMR1-accociated disorders may share common underl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020321137 |
| _version_ | 1819141375176736768 |
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| author | Maria Krasteva Yana Koycheva Rositsa Racheva Teodora Taseva Tsveta Raycheva Stiliana Simeonova Boryan Andreev |
| author_facet | Maria Krasteva Yana Koycheva Rositsa Racheva Teodora Taseva Tsveta Raycheva Stiliana Simeonova Boryan Andreev |
| author_sort | Maria Krasteva |
| collection | DOAJ |
| description | FMR1 gene (fragile X mental retardation 1) represents a genetic and epigenetic factor in a number of human diseases. Though the role of FMR1 gene in substance use disorders (SUDs) is not well studied, a number of investigations indicate that SUDs and FMR1-accociated disorders may share common underlying mechanisms. We examined the relative FMR1 mRNA levels and their sex-distribution in leukocytes from patients with alcohol and drug dependence compared to healthy controls. The study included 44 participants, 16 with alcohol dependence (mean age 43, 10 males and 6 females), 17 with drug dependence (mean age 41, 12 males and 5 females) and 11 healthy controls (mean age 47, 5 males and 6 females). Participants donated 5–6 ml of blood and completed a specialized questionnaire. Total RNA was isolated and cDNA was synthesized and used as a template for qRT-PCR analysis. The studied persons with alcohol and drug dependence share common socio-demographic and substance-use related characteristics. Significant FMR1 down-regulation was observed in the alcohol dependent group (25 % decrease; p = 0.005). Sex-associated analysis revealed that FMR1 down-regulation was primarily in alcohol-dependent men (40% decrease; p = 0.001) and did not reach significance in women. A similar sex-dependent pattern was observed among drug-dependent individuals. Drug-dependent men had significantly lower FMR1 mRNA levels (24% decrease; p = 0.015) compared with controls, while no significant difference was observed in drug-dependent females. These data indicate FMR1 mRNA down-regulation in persons with alcohol- and drug-dependence, relative to controls, is sex-dependent. This implies a role for FMR1 in substance use disorders. These findings require confirmation by including protein measures and the recruitment of larger cohorts. |
| first_indexed | 2024-12-22T11:53:27Z |
| format | Article |
| id | doaj.art-808db35a7d7042439e36b6a3f46e7428 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-22T11:53:27Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-808db35a7d7042439e36b6a3f46e74282022-12-21T18:26:55ZengElsevierHeliyon2405-84402020-10-01610e05270Decreased FMR1 mRNA levels found in men with substance use disordersMaria Krasteva0Yana Koycheva1Rositsa Racheva2Teodora Taseva3Tsveta Raycheva4Stiliana Simeonova5Boryan Andreev6Institute of Plant Physiology and Genetics, Laboratory of Genome Dynamics and Stability, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bldg. 21, 1113 Sofia, Bulgaria; Corresponding author.Institute of Plant Physiology and Genetics, Laboratory of Genome Dynamics and Stability, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaInstitute for Population and Human Studies, Department of Psychology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bldg. 6, 1113 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Laboratory of Genome Dynamics and Stability, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaNational Center for Public Health and Analyses, Department of Addictions, Acad. Ivan Ev. Geshov Street 15, 1431 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Laboratory of Genome Dynamics and Stability, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bldg. 21, 1113 Sofia, BulgariaInstitute for Population and Human Studies, Department of Psychology, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bldg. 6, 1113 Sofia, BulgariaFMR1 gene (fragile X mental retardation 1) represents a genetic and epigenetic factor in a number of human diseases. Though the role of FMR1 gene in substance use disorders (SUDs) is not well studied, a number of investigations indicate that SUDs and FMR1-accociated disorders may share common underlying mechanisms. We examined the relative FMR1 mRNA levels and their sex-distribution in leukocytes from patients with alcohol and drug dependence compared to healthy controls. The study included 44 participants, 16 with alcohol dependence (mean age 43, 10 males and 6 females), 17 with drug dependence (mean age 41, 12 males and 5 females) and 11 healthy controls (mean age 47, 5 males and 6 females). Participants donated 5–6 ml of blood and completed a specialized questionnaire. Total RNA was isolated and cDNA was synthesized and used as a template for qRT-PCR analysis. The studied persons with alcohol and drug dependence share common socio-demographic and substance-use related characteristics. Significant FMR1 down-regulation was observed in the alcohol dependent group (25 % decrease; p = 0.005). Sex-associated analysis revealed that FMR1 down-regulation was primarily in alcohol-dependent men (40% decrease; p = 0.001) and did not reach significance in women. A similar sex-dependent pattern was observed among drug-dependent individuals. Drug-dependent men had significantly lower FMR1 mRNA levels (24% decrease; p = 0.015) compared with controls, while no significant difference was observed in drug-dependent females. These data indicate FMR1 mRNA down-regulation in persons with alcohol- and drug-dependence, relative to controls, is sex-dependent. This implies a role for FMR1 in substance use disorders. These findings require confirmation by including protein measures and the recruitment of larger cohorts.http://www.sciencedirect.com/science/article/pii/S2405844020321137GeneticsEpigeneticsPublic healthClinical geneticsPsychiatryClinical research |
| spellingShingle | Maria Krasteva Yana Koycheva Rositsa Racheva Teodora Taseva Tsveta Raycheva Stiliana Simeonova Boryan Andreev Decreased FMR1 mRNA levels found in men with substance use disorders Heliyon Genetics Epigenetics Public health Clinical genetics Psychiatry Clinical research |
| title | Decreased FMR1 mRNA levels found in men with substance use disorders |
| title_full | Decreased FMR1 mRNA levels found in men with substance use disorders |
| title_fullStr | Decreased FMR1 mRNA levels found in men with substance use disorders |
| title_full_unstemmed | Decreased FMR1 mRNA levels found in men with substance use disorders |
| title_short | Decreased FMR1 mRNA levels found in men with substance use disorders |
| title_sort | decreased fmr1 mrna levels found in men with substance use disorders |
| topic | Genetics Epigenetics Public health Clinical genetics Psychiatry Clinical research |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020321137 |
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