Rosiglitazone promotes glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway
Abstract The study aimed to explore the effects of rosiglitazone on glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway. The experiment was divided into five groups: normal starch group (32%, LC), high starch group (53%, HC), high starch +rosiglitazone group 1 (10 mg/kg, R1),...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-03-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14765 |
| _version_ | 1818910728401190912 |
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| author | Dong‐Yan Guan Hui‐Wen Sun Ji‐Ting Wang Zhen Wang Yang Li Hao‐Jun Han Xiang Li Ting‐Ting Fang |
| author_facet | Dong‐Yan Guan Hui‐Wen Sun Ji‐Ting Wang Zhen Wang Yang Li Hao‐Jun Han Xiang Li Ting‐Ting Fang |
| author_sort | Dong‐Yan Guan |
| collection | DOAJ |
| description | Abstract The study aimed to explore the effects of rosiglitazone on glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway. The experiment was divided into five groups: normal starch group (32%, LC), high starch group (53%, HC), high starch +rosiglitazone group 1 (10 mg/kg, R1), high starch + rosiglitazone group 2 (20 mg/kg, R2), and high starch + rosiglitazone group 3 (30 mg/kg, R3). The results showed that a high starch diet supplemented with 10–20 mg/kg rosiglitazone had a better specific growth rate and protein efficiency that was beneficial for the growth of the tilapia. Rosiglitazone had no significant effect on the contents of crude lipid, crude protein, crude ash, and moisture of the whole fish body (p > 0.05). The contents of triglycerides and total cholesterol in the R1, R2, and R3 groups were lower than those in the HC group. The levels of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in R1 and R2 groups were significantly lower than those in the HC groups (p < 0.05). However, the GOT and GPT levels in the R3 groups were significantly higher than those in the R1 and R2 groups (p < 0.05). With an increase in the rosiglitazone concentration, the contents of serum glucose, insulin, and hepatic glycogen in the R1, R2, and R3 groups decreased gradually. Meanwhile, the muscle glycogen content in the R1, R2, and R3 groups increased gradually. The mRNA expression of the IRS‐1, PI3K, GLUT‐4, and Akt proteins in the R1, R2, and R3 groups was significantly higher than that in the HC group (p < 0.05). Compared with the HC group, the expression of the GSK‐3 mRNA in the R1, R2, and R3 groups was significantly reduced (p < 0.05). The protein expression of p‐Akt in the R1 and R2 groups was higher than that in the HC group (p > 0.05). The protein expression of p‐GSK‐3β in the R1 and R2 groups was significantly higher than that in the HC group (p < 0.05). In conclusion, a high starch diet supplemented with rosiglitazone can improve growth, enhance the serum biochemical indices, and increase the muscle glycogen content in the GIFT tilapia. It benefits in upregulating the IRS‐1, PI3K, and GLUT‐4 mRNA levels in the skeletal muscle and promotes glucose uptake. Meanwhile, the phosphorylation of Akt and GSK‐3β increased significantly and resulted in the inactivation of GSK‐3β and alleviation of insulin resistance. |
| first_indexed | 2024-12-19T22:47:25Z |
| format | Article |
| id | doaj.art-808ebe192ce54c53b4d424b2acae509b |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-19T22:47:25Z |
| publishDate | 2021-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-808ebe192ce54c53b4d424b2acae509b2022-12-21T20:02:55ZengWileyPhysiological Reports2051-817X2021-03-0195n/an/a10.14814/phy2.14765Rosiglitazone promotes glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathwayDong‐Yan Guan0Hui‐Wen Sun1Ji‐Ting Wang2Zhen Wang3Yang Li4Hao‐Jun Han5Xiang Li6Ting‐Ting Fang7Shandong Provincial Key Lab. of Animal Biotechnology and Disease Control and Prevention Lab of Aquatic Animal Nutrition & Environmental Health Shandong Agricultural University Taian City Shandong Province ChinaShandong Provincial Key Lab. of Animal Biotechnology and Disease Control and Prevention Lab of Aquatic Animal Nutrition & Environmental Health Shandong Agricultural University Taian City Shandong Province ChinaShandong Provincial Key Lab. of Animal Biotechnology and Disease Control and Prevention Lab of Aquatic Animal Nutrition & Environmental Health Shandong Agricultural University Taian City Shandong Province ChinaShandong Provincial Key Lab. of Animal Biotechnology and Disease Control and Prevention Lab of Aquatic Animal Nutrition & Environmental Health Shandong Agricultural University Taian City Shandong Province ChinaShandong Provincial Key Lab. of Animal Biotechnology and Disease Control and Prevention Lab of Aquatic Animal Nutrition & Environmental Health Shandong Agricultural University Taian City Shandong Province ChinaShandong Provincial Key Lab. of Animal Biotechnology and Disease Control and Prevention Lab of Aquatic Animal Nutrition & Environmental Health Shandong Agricultural University Taian City Shandong Province ChinaShandong Provincial Key Lab. of Animal Biotechnology and Disease Control and Prevention Lab of Aquatic Animal Nutrition & Environmental Health Shandong Agricultural University Taian City Shandong Province ChinaShandong Provincial Key Lab. of Animal Biotechnology and Disease Control and Prevention Lab of Aquatic Animal Nutrition & Environmental Health Shandong Agricultural University Taian City Shandong Province ChinaAbstract The study aimed to explore the effects of rosiglitazone on glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway. The experiment was divided into five groups: normal starch group (32%, LC), high starch group (53%, HC), high starch +rosiglitazone group 1 (10 mg/kg, R1), high starch + rosiglitazone group 2 (20 mg/kg, R2), and high starch + rosiglitazone group 3 (30 mg/kg, R3). The results showed that a high starch diet supplemented with 10–20 mg/kg rosiglitazone had a better specific growth rate and protein efficiency that was beneficial for the growth of the tilapia. Rosiglitazone had no significant effect on the contents of crude lipid, crude protein, crude ash, and moisture of the whole fish body (p > 0.05). The contents of triglycerides and total cholesterol in the R1, R2, and R3 groups were lower than those in the HC group. The levels of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in R1 and R2 groups were significantly lower than those in the HC groups (p < 0.05). However, the GOT and GPT levels in the R3 groups were significantly higher than those in the R1 and R2 groups (p < 0.05). With an increase in the rosiglitazone concentration, the contents of serum glucose, insulin, and hepatic glycogen in the R1, R2, and R3 groups decreased gradually. Meanwhile, the muscle glycogen content in the R1, R2, and R3 groups increased gradually. The mRNA expression of the IRS‐1, PI3K, GLUT‐4, and Akt proteins in the R1, R2, and R3 groups was significantly higher than that in the HC group (p < 0.05). Compared with the HC group, the expression of the GSK‐3 mRNA in the R1, R2, and R3 groups was significantly reduced (p < 0.05). The protein expression of p‐Akt in the R1 and R2 groups was higher than that in the HC group (p > 0.05). The protein expression of p‐GSK‐3β in the R1 and R2 groups was significantly higher than that in the HC group (p < 0.05). In conclusion, a high starch diet supplemented with rosiglitazone can improve growth, enhance the serum biochemical indices, and increase the muscle glycogen content in the GIFT tilapia. It benefits in upregulating the IRS‐1, PI3K, and GLUT‐4 mRNA levels in the skeletal muscle and promotes glucose uptake. Meanwhile, the phosphorylation of Akt and GSK‐3β increased significantly and resulted in the inactivation of GSK‐3β and alleviation of insulin resistance.https://doi.org/10.14814/phy2.14765GIFT tilapiaglucose metabolismgrowthPI3K/Aktrosiglitazone |
| spellingShingle | Dong‐Yan Guan Hui‐Wen Sun Ji‐Ting Wang Zhen Wang Yang Li Hao‐Jun Han Xiang Li Ting‐Ting Fang Rosiglitazone promotes glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway Physiological Reports GIFT tilapia glucose metabolism growth PI3K/Akt rosiglitazone |
| title | Rosiglitazone promotes glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway |
| title_full | Rosiglitazone promotes glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway |
| title_fullStr | Rosiglitazone promotes glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway |
| title_full_unstemmed | Rosiglitazone promotes glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway |
| title_short | Rosiglitazone promotes glucose metabolism of GIFT tilapia based on the PI3K/Akt signaling pathway |
| title_sort | rosiglitazone promotes glucose metabolism of gift tilapia based on the pi3k akt signaling pathway |
| topic | GIFT tilapia glucose metabolism growth PI3K/Akt rosiglitazone |
| url | https://doi.org/10.14814/phy2.14765 |
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