Identification of Crotonylation Metabolism Signature Predicting Overall Survival for Clear Cell Renal Cell Carcinoma
Background. Immunotherapy shows promise in treating cancer by leveraging the immune system to combat cancer cells. However, the influence of crotonylation metabolism on the prognosis and tumor environment in ccRCC patients is not fully understood. Methods. We conducted various systematic analyses, i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi-Wiley
2023-01-01
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| Series: | International Journal of Clinical Practice |
| Online Access: | http://dx.doi.org/10.1155/2023/5558034 |
| _version_ | 1797403712938835968 |
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| author | Jie Zheng Yingqing Liu Kai Wei Jiewu Shi Lin Li Xuefeng Jiang Lingsong Tao |
| author_facet | Jie Zheng Yingqing Liu Kai Wei Jiewu Shi Lin Li Xuefeng Jiang Lingsong Tao |
| author_sort | Jie Zheng |
| collection | DOAJ |
| description | Background. Immunotherapy shows promise in treating cancer by leveraging the immune system to combat cancer cells. However, the influence of crotonylation metabolism on the prognosis and tumor environment in ccRCC patients is not fully understood. Methods. We conducted various systematic analyses, including prognosis and cluster analyses, to investigate the role of KAT2A in immunotherapy. We used qRT-PCR to compare KAT2A expression in cancer and adjacent tissues and among different cell lines. Additionally, we employed Cell Counting Kit-8, wound healing, and Transwell chamber assays to assess changes in the proliferative and metastatic ability of A498 and 786-O cells. Results. We identified three clusters related to crotonylation metabolism, each with distinct prognosis and immune characteristics in ccRCC. We categorized CT1 as immune-inflamed, CT2 as immune-excluded, and CR3 as immune-desert. A new system, CRS, emerged as an effective predictor of patient outcomes with differing immune characteristics. Moreover, qRT-PCR revealed elevated KAT2A levels in ccRCC tissues and cell lines. KAT2A was found to promote ccRCC and correlate significantly with immunosuppressive elements and checkpoints. Reducing KAT2A expression hindered ccRCC cell growth and metastasis. Conclusion. Our study highlights the critical role of crotonylation metabolism in cancer development and progression, particularly its link to poor prognosis. CRS proves to be an accurate predictor of patient outcomes and immune features in ccRCC. KAT2A shows strong associations with clinical factors and the immunosuppressive environment, suggesting potential for innovative immunotherapies in ccRCC treatment. |
| first_indexed | 2024-03-09T02:43:23Z |
| format | Article |
| id | doaj.art-808fc5fe59174c179aa3b0caed6a5d81 |
| institution | Directory Open Access Journal |
| issn | 1742-1241 |
| language | English |
| last_indexed | 2024-03-09T02:43:23Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi-Wiley |
| record_format | Article |
| series | International Journal of Clinical Practice |
| spelling | doaj.art-808fc5fe59174c179aa3b0caed6a5d812023-12-06T00:00:04ZengHindawi-WileyInternational Journal of Clinical Practice1742-12412023-01-01202310.1155/2023/5558034Identification of Crotonylation Metabolism Signature Predicting Overall Survival for Clear Cell Renal Cell CarcinomaJie Zheng0Yingqing Liu1Kai Wei2Jiewu Shi3Lin Li4Xuefeng Jiang5Lingsong Tao6Department of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyBackground. Immunotherapy shows promise in treating cancer by leveraging the immune system to combat cancer cells. However, the influence of crotonylation metabolism on the prognosis and tumor environment in ccRCC patients is not fully understood. Methods. We conducted various systematic analyses, including prognosis and cluster analyses, to investigate the role of KAT2A in immunotherapy. We used qRT-PCR to compare KAT2A expression in cancer and adjacent tissues and among different cell lines. Additionally, we employed Cell Counting Kit-8, wound healing, and Transwell chamber assays to assess changes in the proliferative and metastatic ability of A498 and 786-O cells. Results. We identified three clusters related to crotonylation metabolism, each with distinct prognosis and immune characteristics in ccRCC. We categorized CT1 as immune-inflamed, CT2 as immune-excluded, and CR3 as immune-desert. A new system, CRS, emerged as an effective predictor of patient outcomes with differing immune characteristics. Moreover, qRT-PCR revealed elevated KAT2A levels in ccRCC tissues and cell lines. KAT2A was found to promote ccRCC and correlate significantly with immunosuppressive elements and checkpoints. Reducing KAT2A expression hindered ccRCC cell growth and metastasis. Conclusion. Our study highlights the critical role of crotonylation metabolism in cancer development and progression, particularly its link to poor prognosis. CRS proves to be an accurate predictor of patient outcomes and immune features in ccRCC. KAT2A shows strong associations with clinical factors and the immunosuppressive environment, suggesting potential for innovative immunotherapies in ccRCC treatment.http://dx.doi.org/10.1155/2023/5558034 |
| spellingShingle | Jie Zheng Yingqing Liu Kai Wei Jiewu Shi Lin Li Xuefeng Jiang Lingsong Tao Identification of Crotonylation Metabolism Signature Predicting Overall Survival for Clear Cell Renal Cell Carcinoma International Journal of Clinical Practice |
| title | Identification of Crotonylation Metabolism Signature Predicting Overall Survival for Clear Cell Renal Cell Carcinoma |
| title_full | Identification of Crotonylation Metabolism Signature Predicting Overall Survival for Clear Cell Renal Cell Carcinoma |
| title_fullStr | Identification of Crotonylation Metabolism Signature Predicting Overall Survival for Clear Cell Renal Cell Carcinoma |
| title_full_unstemmed | Identification of Crotonylation Metabolism Signature Predicting Overall Survival for Clear Cell Renal Cell Carcinoma |
| title_short | Identification of Crotonylation Metabolism Signature Predicting Overall Survival for Clear Cell Renal Cell Carcinoma |
| title_sort | identification of crotonylation metabolism signature predicting overall survival for clear cell renal cell carcinoma |
| url | http://dx.doi.org/10.1155/2023/5558034 |
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