Flavonoids and coumarins from Hieracium pilosella L. (Asteraceae)
Typical chromatographic methods were successfully applied to isolate nine flavonoid compounds and two coumarin glycosides from the inflorescences and the herb of Hieracium pilosella L. Repeated column chromatography, occasionally paper chromatography and recrystallization made the separation of thre...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2011-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/387 |
| _version_ | 1818304769387659264 |
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| author | Tadeusz Krzaczek Monika Gawrońska-Grzywacz |
| author_facet | Tadeusz Krzaczek Monika Gawrońska-Grzywacz |
| author_sort | Tadeusz Krzaczek |
| collection | DOAJ |
| description | Typical chromatographic methods were successfully applied to isolate nine flavonoid compounds and two coumarin glycosides from the inflorescences and the herb of Hieracium pilosella L. Repeated column chromatography, occasionally paper chromatography and recrystallization made the separation of three flavonoid aglycones and six glycosides - possible. Coumarin glycosides were isolated by preparative thin layer chromatography. Subsequent UV, NMR and MS analyses have led to identification of the following flavonoid derivatives: known for the species - apigenin, luteolin, luteolin 7-O- ß-glucopyranoside, luteolin 4’-O-ß-glucopyranoside, isoetin 7-O-ß- -glucopyranoside, isoetin 4’-O-ß-glucuronide and new for the species – kaempferol 3-methyl ether and apigenin 7-O-ß-glucopyranoside. Third isoetin glycoside contained two different sugar moieties: xylose and glucose, probably attached to the hydroxyl groups at C-4’ or C-4’ and C-2’(or 5’) of an aglycone. Umbelliferone 7-O-ß-glucopyranoside (skimmin) and new for the genus Hieracium esculetin 7-O-ß-glucopyranoside (cichoriin) were determined by NMR and MS methods. |
| first_indexed | 2024-12-13T06:15:57Z |
| format | Article |
| id | doaj.art-8092b10e53d743fbb54fe0dc77ecd1d4 |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-13T06:15:57Z |
| publishDate | 2011-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-8092b10e53d743fbb54fe0dc77ecd1d42022-12-21T23:56:58ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802011-01-0178318919510.5586/asbp.2009.023199Flavonoids and coumarins from Hieracium pilosella L. (Asteraceae)Tadeusz Krzaczek0Monika Gawrońska-Grzywacz1Medical University of LublinMedical University of LublinTypical chromatographic methods were successfully applied to isolate nine flavonoid compounds and two coumarin glycosides from the inflorescences and the herb of Hieracium pilosella L. Repeated column chromatography, occasionally paper chromatography and recrystallization made the separation of three flavonoid aglycones and six glycosides - possible. Coumarin glycosides were isolated by preparative thin layer chromatography. Subsequent UV, NMR and MS analyses have led to identification of the following flavonoid derivatives: known for the species - apigenin, luteolin, luteolin 7-O- ß-glucopyranoside, luteolin 4’-O-ß-glucopyranoside, isoetin 7-O-ß- -glucopyranoside, isoetin 4’-O-ß-glucuronide and new for the species – kaempferol 3-methyl ether and apigenin 7-O-ß-glucopyranoside. Third isoetin glycoside contained two different sugar moieties: xylose and glucose, probably attached to the hydroxyl groups at C-4’ or C-4’ and C-2’(or 5’) of an aglycone. Umbelliferone 7-O-ß-glucopyranoside (skimmin) and new for the genus Hieracium esculetin 7-O-ß-glucopyranoside (cichoriin) were determined by NMR and MS methods.https://pbsociety.org.pl/journals/index.php/asbp/article/view/387Hieracium pilosellaflavonoidscoumarin glycosidesLCNMRLSI MS |
| spellingShingle | Tadeusz Krzaczek Monika Gawrońska-Grzywacz Flavonoids and coumarins from Hieracium pilosella L. (Asteraceae) Acta Societatis Botanicorum Poloniae Hieracium pilosella flavonoids coumarin glycosides LC NMR LSI MS |
| title | Flavonoids and coumarins from Hieracium pilosella L. (Asteraceae) |
| title_full | Flavonoids and coumarins from Hieracium pilosella L. (Asteraceae) |
| title_fullStr | Flavonoids and coumarins from Hieracium pilosella L. (Asteraceae) |
| title_full_unstemmed | Flavonoids and coumarins from Hieracium pilosella L. (Asteraceae) |
| title_short | Flavonoids and coumarins from Hieracium pilosella L. (Asteraceae) |
| title_sort | flavonoids and coumarins from hieracium pilosella l asteraceae |
| topic | Hieracium pilosella flavonoids coumarin glycosides LC NMR LSI MS |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/387 |
| work_keys_str_mv | AT tadeuszkrzaczek flavonoidsandcoumarinsfromhieraciumpilosellalasteraceae AT monikagawronskagrzywacz flavonoidsandcoumarinsfromhieraciumpilosellalasteraceae |