Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic isolates in southwestern Iran
Objectives This study was undertaken to evaluate AmpC β-lactamase-producing Escherichia coli urine isolates and to characterize the frequency of plasmid-mediated AmpC (pAmpC)-encoding genes. Methods Antimicrobial susceptibility tests were performed using the disk diffusion technique. AmpC β-lactamas...
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| Format: | Article |
| Language: | English |
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Korea Disease Control and Prevention Agency
2021-12-01
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| Series: | Osong Public Health and Research Perspectives |
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| Online Access: | http://ophrp.org/upload/pdf/j-phrp-2021-0272.pdf |
| _version_ | 1827851415690674176 |
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| author | Nabi Jomehzadeh Khadijeh Ahmadi Zahra Rahmani |
| author_facet | Nabi Jomehzadeh Khadijeh Ahmadi Zahra Rahmani |
| author_sort | Nabi Jomehzadeh |
| collection | DOAJ |
| description | Objectives This study was undertaken to evaluate AmpC β-lactamase-producing Escherichia coli urine isolates and to characterize the frequency of plasmid-mediated AmpC (pAmpC)-encoding genes. Methods Antimicrobial susceptibility tests were performed using the disk diffusion technique. AmpC β-lactamase production was assessed with a phenotypic inhibitor-based method. The presence of 6 pAmpC-encoding cluster genes was detected by multiplex polymerase chain reaction (PCR). Results The proportion of antibiotic resistance of E. coli isolates ranged from 7.4% to 90.5%, and more than half (51.6%) of the total isolates were multidrug-resistant (MDR). Among the 95 E. coli isolates, 60 (63.2%) were found to be cefoxitin-resistant, but only 14 (14.7%) isolates were confirmed as AmpC β-lactamase-producers. In the PCR assay, pAmpC-encoding genes were found in 15 (15.8%) isolates, and blaDHA was the most prevalent type. However, blaFOX, blaMOX, and blaACC genes were not detected in the isolates. Conclusion Our findings contributed valuable information concerning antibiotic resistance, confirmatory phenotypic testing for AmpC production, and pAmpC β-lactamase gene content in E. coli isolates in southwestern Iran. The level of MDR recorded in AmpC-producing strains of this study was worrying; therefore, implementing strong infection control approaches to reduce the MDR burden is recommended. |
| first_indexed | 2024-03-12T10:32:48Z |
| format | Article |
| id | doaj.art-8092b66331ac43078db264f9a8f3cca5 |
| institution | Directory Open Access Journal |
| issn | 2210-9099 2210-9110 |
| language | English |
| last_indexed | 2024-03-12T10:32:48Z |
| publishDate | 2021-12-01 |
| publisher | Korea Disease Control and Prevention Agency |
| record_format | Article |
| series | Osong Public Health and Research Perspectives |
| spelling | doaj.art-8092b66331ac43078db264f9a8f3cca52023-09-02T09:06:24ZengKorea Disease Control and Prevention AgencyOsong Public Health and Research Perspectives2210-90992210-91102021-12-0112639039510.24171/j.phrp.2021.0272640Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic isolates in southwestern IranNabi Jomehzadeh0Khadijeh Ahmadi1Zahra Rahmani2 Department of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran Department of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran Abadan University of Medical Sciences, Abadan, IranObjectives This study was undertaken to evaluate AmpC β-lactamase-producing Escherichia coli urine isolates and to characterize the frequency of plasmid-mediated AmpC (pAmpC)-encoding genes. Methods Antimicrobial susceptibility tests were performed using the disk diffusion technique. AmpC β-lactamase production was assessed with a phenotypic inhibitor-based method. The presence of 6 pAmpC-encoding cluster genes was detected by multiplex polymerase chain reaction (PCR). Results The proportion of antibiotic resistance of E. coli isolates ranged from 7.4% to 90.5%, and more than half (51.6%) of the total isolates were multidrug-resistant (MDR). Among the 95 E. coli isolates, 60 (63.2%) were found to be cefoxitin-resistant, but only 14 (14.7%) isolates were confirmed as AmpC β-lactamase-producers. In the PCR assay, pAmpC-encoding genes were found in 15 (15.8%) isolates, and blaDHA was the most prevalent type. However, blaFOX, blaMOX, and blaACC genes were not detected in the isolates. Conclusion Our findings contributed valuable information concerning antibiotic resistance, confirmatory phenotypic testing for AmpC production, and pAmpC β-lactamase gene content in E. coli isolates in southwestern Iran. The level of MDR recorded in AmpC-producing strains of this study was worrying; therefore, implementing strong infection control approaches to reduce the MDR burden is recommended.http://ophrp.org/upload/pdf/j-phrp-2021-0272.pdfampc multidrug resistanceurinary tract infections |
| spellingShingle | Nabi Jomehzadeh Khadijeh Ahmadi Zahra Rahmani Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic isolates in southwestern Iran Osong Public Health and Research Perspectives ampc multidrug resistance urinary tract infections |
| title | Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic isolates in southwestern Iran |
| title_full | Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic isolates in southwestern Iran |
| title_fullStr | Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic isolates in southwestern Iran |
| title_full_unstemmed | Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic isolates in southwestern Iran |
| title_short | Prevalence of plasmid-mediated AmpC β-lactamases among uropathogenic isolates in southwestern Iran |
| title_sort | prevalence of plasmid mediated ampc β lactamases among uropathogenic isolates in southwestern iran |
| topic | ampc multidrug resistance urinary tract infections |
| url | http://ophrp.org/upload/pdf/j-phrp-2021-0272.pdf |
| work_keys_str_mv | AT nabijomehzadeh prevalenceofplasmidmediatedampcblactamasesamonguropathogenicisolatesinsouthwesterniran AT khadijehahmadi prevalenceofplasmidmediatedampcblactamasesamonguropathogenicisolatesinsouthwesterniran AT zahrarahmani prevalenceofplasmidmediatedampcblactamasesamonguropathogenicisolatesinsouthwesterniran |