Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice
The purpose of the present study was to investigate the in vitro and in vivo effects of aluminum sulfate on delta-aminolevulinic acid dehydratase (ALA-D) activity from the brain, liver and kidney of adult mice (Swiss albine). In vitro experiments showed that the aluminum sulfate concentration needed...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1999-06-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600012&tlng=en |
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| author | M.R.C. Schetinger C.D. Bonan V.M. Morsch D. Bohrer L.M. Valentim S.R. Rodrigues |
| author_facet | M.R.C. Schetinger C.D. Bonan V.M. Morsch D. Bohrer L.M. Valentim S.R. Rodrigues |
| author_sort | M.R.C. Schetinger |
| collection | DOAJ |
| description | The purpose of the present study was to investigate the in vitro and in vivo effects of aluminum sulfate on delta-aminolevulinic acid dehydratase (ALA-D) activity from the brain, liver and kidney of adult mice (Swiss albine). In vitro experiments showed that the aluminum sulfate concentration needed to inhibit the enzyme activity was 1.0-5.0 mM (N = 3) in brain, 4.0-5.0 mM (N = 3) in liver and 0.0-5.0 mM (N = 3) in kidney. The in vivo experiments were performed on three groups for one month: 1) control animals (N = 8); 2) animals treated with 1 g% (34 mM) sodium citrate (N = 8) and 3) animals treated with 1 g% (34 mM) sodium citrate plus 3.3 g% (49.5 mM) aluminum sulfate (N = 8). Exposure to aluminum sulfate in drinking water inhibited ALA-D activity in kidney (23.3 ± 3.7%, mean ± SEM, P<0.05 compared to control), but enhanced it in liver (31.2 ± 15.0%, mean ± SEM, P<0.05). The concentrations of aluminum in the brain, liver and kidney of adult mice were determined by graphite furnace atomic absorption spectrometry. The aluminum concentrations increased significantly in the liver (527 ± 3.9%, mean ± SEM, P<0.05) and kidney (283 ± 1.7%, mean ± SEM, P<0.05) but did not change in the brain of aluminum-exposed mice. One of the most important and striking observations was the increase in hepatic aluminum concentration in the mice treated only with 1 g% sodium citrate (34 mM) (217 ± 1.5%, mean ± SEM, P<0.05 compared to control). These results show that aluminum interferes with delta-aminolevulinate dehydratase activity in vitro and in vivo. The accumulation of this element was in the order: liver > kidney > brain. Furthermore, aluminum had only inhibitory properties in vitro, while in vivo it inhibited or stimulated the enzyme depending on the organ studied. |
| first_indexed | 2024-04-11T16:43:53Z |
| format | Article |
| id | doaj.art-8093b916229d496b91ec97a589b7d6df |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-11T16:43:53Z |
| publishDate | 1999-06-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-8093b916229d496b91ec97a589b7d6df2022-12-22T04:13:36ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X1999-06-0132676176610.1590/S0100-879X1999000600012Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult miceM.R.C. Schetinger0C.D. Bonan1V.M. Morsch2D. Bohrer3L.M. Valentim4S.R. Rodrigues5Universidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaThe purpose of the present study was to investigate the in vitro and in vivo effects of aluminum sulfate on delta-aminolevulinic acid dehydratase (ALA-D) activity from the brain, liver and kidney of adult mice (Swiss albine). In vitro experiments showed that the aluminum sulfate concentration needed to inhibit the enzyme activity was 1.0-5.0 mM (N = 3) in brain, 4.0-5.0 mM (N = 3) in liver and 0.0-5.0 mM (N = 3) in kidney. The in vivo experiments were performed on three groups for one month: 1) control animals (N = 8); 2) animals treated with 1 g% (34 mM) sodium citrate (N = 8) and 3) animals treated with 1 g% (34 mM) sodium citrate plus 3.3 g% (49.5 mM) aluminum sulfate (N = 8). Exposure to aluminum sulfate in drinking water inhibited ALA-D activity in kidney (23.3 ± 3.7%, mean ± SEM, P<0.05 compared to control), but enhanced it in liver (31.2 ± 15.0%, mean ± SEM, P<0.05). The concentrations of aluminum in the brain, liver and kidney of adult mice were determined by graphite furnace atomic absorption spectrometry. The aluminum concentrations increased significantly in the liver (527 ± 3.9%, mean ± SEM, P<0.05) and kidney (283 ± 1.7%, mean ± SEM, P<0.05) but did not change in the brain of aluminum-exposed mice. One of the most important and striking observations was the increase in hepatic aluminum concentration in the mice treated only with 1 g% sodium citrate (34 mM) (217 ± 1.5%, mean ± SEM, P<0.05 compared to control). These results show that aluminum interferes with delta-aminolevulinate dehydratase activity in vitro and in vivo. The accumulation of this element was in the order: liver > kidney > brain. Furthermore, aluminum had only inhibitory properties in vitro, while in vivo it inhibited or stimulated the enzyme depending on the organ studied.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600012&tlng=enaluminum sulfatealuminum determinationdelta-aminolevulinate dehydratase |
| spellingShingle | M.R.C. Schetinger C.D. Bonan V.M. Morsch D. Bohrer L.M. Valentim S.R. Rodrigues Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice Brazilian Journal of Medical and Biological Research aluminum sulfate aluminum determination delta-aminolevulinate dehydratase |
| title | Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice |
| title_full | Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice |
| title_fullStr | Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice |
| title_full_unstemmed | Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice |
| title_short | Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice |
| title_sort | effects of aluminum sulfate on delta aminolevulinate dehydratase from kidney brain and liver of adult mice |
| topic | aluminum sulfate aluminum determination delta-aminolevulinate dehydratase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600012&tlng=en |
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