Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis
Objective: To assess the effect of metalloestrogen cadmium (Cd) on the proliferation of endometrial stromal cells (ESC) isolated from the eutopic endometrium of women with endometriosis Materials and Methods: ESC were isolated from eutopic endometrial samples from 10 women with endometriosis and 10...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013-12-01
|
| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1028455913001782 |
| _version_ | 1818179314730926080 |
|---|---|
| author | Nalinda Silva Kamani Tennekoon Hemantha Senanayake Sameera Samarakoon |
| author_facet | Nalinda Silva Kamani Tennekoon Hemantha Senanayake Sameera Samarakoon |
| author_sort | Nalinda Silva |
| collection | DOAJ |
| description | Objective: To assess the effect of metalloestrogen cadmium (Cd) on the proliferation of endometrial stromal cells (ESC) isolated from the eutopic endometrium of women with endometriosis
Materials and Methods: ESC were isolated from eutopic endometrial samples from 10 women with endometriosis and 10 women without endometriosis. ESC cultures were established and maintained in RPMI medium. Cultures were treated with Cd at a concentration of 10−6 M and after 24 hours and 48 hours, cell number was counted using the Neubauer hemocytometer to calculate the relative cell proliferation. At 48 hours, the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay was used to test the effect of different concentrations (10−8 M to 10−3 M) of Cd on ESC cultures. Relative cell proliferation and MTT assay results were analyzed with ANOVA.
Results: At 48 hours, Cd-induced ESC proliferation was higher (p = 0.02) in women with endometriosis than in women without endometriosis, which was confirmed by the MTT assay.
Conclusion: The results of this study demonstrate the ability of metalloestrogen Cd to induce the proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis. |
| first_indexed | 2024-12-11T21:01:55Z |
| format | Article |
| id | doaj.art-8096b08254d04b89968519e731b77e8a |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-12-11T21:01:55Z |
| publishDate | 2013-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-8096b08254d04b89968519e731b77e8a2022-12-22T00:50:57ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592013-12-0152454054510.1016/j.tjog.2013.10.015Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosisNalinda Silva0Kamani Tennekoon1Hemantha Senanayake2Sameera Samarakoon3Department of Physiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Sri LankaInstitute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri LankaDepartment of Obstetrics and Gynaecology, Faculty of Medicine, University of Colombo, Colombo, Sri LankaInstitute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri LankaObjective: To assess the effect of metalloestrogen cadmium (Cd) on the proliferation of endometrial stromal cells (ESC) isolated from the eutopic endometrium of women with endometriosis Materials and Methods: ESC were isolated from eutopic endometrial samples from 10 women with endometriosis and 10 women without endometriosis. ESC cultures were established and maintained in RPMI medium. Cultures were treated with Cd at a concentration of 10−6 M and after 24 hours and 48 hours, cell number was counted using the Neubauer hemocytometer to calculate the relative cell proliferation. At 48 hours, the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay was used to test the effect of different concentrations (10−8 M to 10−3 M) of Cd on ESC cultures. Relative cell proliferation and MTT assay results were analyzed with ANOVA. Results: At 48 hours, Cd-induced ESC proliferation was higher (p = 0.02) in women with endometriosis than in women without endometriosis, which was confirmed by the MTT assay. Conclusion: The results of this study demonstrate the ability of metalloestrogen Cd to induce the proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis.http://www.sciencedirect.com/science/article/pii/S1028455913001782cadmiumendometrial stromal cellsendometriosismetalloestrogensestrogen receptor |
| spellingShingle | Nalinda Silva Kamani Tennekoon Hemantha Senanayake Sameera Samarakoon Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis Taiwanese Journal of Obstetrics & Gynecology cadmium endometrial stromal cells endometriosis metalloestrogens estrogen receptor |
| title | Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis |
| title_full | Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis |
| title_fullStr | Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis |
| title_full_unstemmed | Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis |
| title_short | Metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis |
| title_sort | metalloestrogen cadmium stimulates proliferation of stromal cells derived from the eutopic endometrium of women with endometriosis |
| topic | cadmium endometrial stromal cells endometriosis metalloestrogens estrogen receptor |
| url | http://www.sciencedirect.com/science/article/pii/S1028455913001782 |
| work_keys_str_mv | AT nalindasilva metalloestrogencadmiumstimulatesproliferationofstromalcellsderivedfromtheeutopicendometriumofwomenwithendometriosis AT kamanitennekoon metalloestrogencadmiumstimulatesproliferationofstromalcellsderivedfromtheeutopicendometriumofwomenwithendometriosis AT hemanthasenanayake metalloestrogencadmiumstimulatesproliferationofstromalcellsderivedfromtheeutopicendometriumofwomenwithendometriosis AT sameerasamarakoon metalloestrogencadmiumstimulatesproliferationofstromalcellsderivedfromtheeutopicendometriumofwomenwithendometriosis |