Frequency and Antibiotic Susceptibility Patterns of <i>Streptococcus agalactiae</i> Strains Isolated from Women in Yaounde, Cameroon
Group B <i>Streptococcus</i> (GBS), a commensal in the body, causes a wide range of infectious diseases. This bacterium is dangerous for pregnant women and their babies, in whom it is responsible for early neonatal bacterial sepsis (EOS). The colonisation levels of GBS and its resistance...
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MDPI AG
2022-11-01
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| Series: | Microbiology Research |
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| author | Cécile Ingrid Djuikoue Paule Dana Djouela Djoulako Rodrigue Kamga Wouambo Rosine Yemetio Foutsa Dorine Ekeu Ngatcheu Teke Apalata |
| author_facet | Cécile Ingrid Djuikoue Paule Dana Djouela Djoulako Rodrigue Kamga Wouambo Rosine Yemetio Foutsa Dorine Ekeu Ngatcheu Teke Apalata |
| author_sort | Cécile Ingrid Djuikoue |
| collection | DOAJ |
| description | Group B <i>Streptococcus</i> (GBS), a commensal in the body, causes a wide range of infectious diseases. This bacterium is dangerous for pregnant women and their babies, in whom it is responsible for early neonatal bacterial sepsis (EOS). The colonisation levels of GBS and its resistance profile to antibiotics provide important information that is useful for orienting prevention strategies. There are few data available on the subject on the determination of resistance phenotypes in Cameroon. We therefore aimed to determine the prevalence of colonisation and antibiotic resistance, including patterns of inducible resistance to clindamycin, of GBS in the city of Yaounde. To achieve this goal, a prospective cross-sectional study with an analytical component was carried out from 28 June to 29 August 2020 at the BIOSANTE laboratory and the Yaounde Gynaeco-Obstetrics and Paediatrics hospital. Vaginal swabs and urine were collected from 163 women. This samples were analysed using 5% defibrinated sheep blood agar and chocolate plus polyvitex agar. The isolates were identified using the morphology of the colony, Gram staining, haemolysis, catalase tests and latex grouping tests. Antibiotic susceptibility testing was carried out by disk diffusion method following the recommendations of the ACFSM 2019. The double disk diffusion method was used to identify isolates with clindamycin-inducible resistance. Our data were analysed with SPSS version 2.1. The results obtained showed that the overall prevalence of colonisation by GBS was 37% (57/163), or 40.3% in non-pregnant women and 59.7% in pregnant women. Pregnancy (<i>p</i>-value = 0.019) and earlier (from the second semester of pregnancy) gestational age (<i>p</i>-value = 0.025) constituted the risk factors of maternal colonisation by GBS. In addition, the strains of GBS were resistant to all 16 antibiotics tested. A D test showed that 64.7% of GBS strains were constitutively resistant to clindamycin. We also note the presence of M phenotypes. As a whole, our results demonstrated that the rate of GBS colonisation in this study was similar to or higher than those in previous reports in Cameroon. All these results indicate that attention should be paid to this bacterium in the monitoring of antimicrobial resistance and in the care of pregnant women and newborns. |
| first_indexed | 2024-03-09T16:05:15Z |
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| id | doaj.art-809a3989df6243bc8b47cac6b99f4369 |
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| publishDate | 2022-11-01 |
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| spelling | doaj.art-809a3989df6243bc8b47cac6b99f43692023-11-24T16:42:55ZengMDPI AGMicrobiology Research2036-74812022-11-0113495496210.3390/microbiolres13040068Frequency and Antibiotic Susceptibility Patterns of <i>Streptococcus agalactiae</i> Strains Isolated from Women in Yaounde, CameroonCécile Ingrid Djuikoue0Paule Dana Djouela Djoulako1Rodrigue Kamga Wouambo2Rosine Yemetio Foutsa3Dorine Ekeu Ngatcheu4Teke Apalata5Microbiology Department, Faculty of Health Sciences, Université des Montagnes, Bangangté BP 208, CameroonDepartment of Microbiology and Parasitology, Faculty of Sciences, University of Buea, Buea P.O. Box 63, CameroonDepartment of Microbiology and Parasitology, Faculty of Sciences, University of Buea, Buea P.O. Box 63, CameroonDepartment of Microbiology and Parasitology, Faculty of Sciences, University of Buea, Buea P.O. Box 63, CameroonMicrobiology Department, Faculty of Health Sciences, Université des Montagnes, Bangangté BP 208, CameroonNational Health Laboratory Services, Faculty of Health Sciences, Walter Sisulu University, Nelson Mandela Drive, Mthatha 5117, South AfricaGroup B <i>Streptococcus</i> (GBS), a commensal in the body, causes a wide range of infectious diseases. This bacterium is dangerous for pregnant women and their babies, in whom it is responsible for early neonatal bacterial sepsis (EOS). The colonisation levels of GBS and its resistance profile to antibiotics provide important information that is useful for orienting prevention strategies. There are few data available on the subject on the determination of resistance phenotypes in Cameroon. We therefore aimed to determine the prevalence of colonisation and antibiotic resistance, including patterns of inducible resistance to clindamycin, of GBS in the city of Yaounde. To achieve this goal, a prospective cross-sectional study with an analytical component was carried out from 28 June to 29 August 2020 at the BIOSANTE laboratory and the Yaounde Gynaeco-Obstetrics and Paediatrics hospital. Vaginal swabs and urine were collected from 163 women. This samples were analysed using 5% defibrinated sheep blood agar and chocolate plus polyvitex agar. The isolates were identified using the morphology of the colony, Gram staining, haemolysis, catalase tests and latex grouping tests. Antibiotic susceptibility testing was carried out by disk diffusion method following the recommendations of the ACFSM 2019. The double disk diffusion method was used to identify isolates with clindamycin-inducible resistance. Our data were analysed with SPSS version 2.1. The results obtained showed that the overall prevalence of colonisation by GBS was 37% (57/163), or 40.3% in non-pregnant women and 59.7% in pregnant women. Pregnancy (<i>p</i>-value = 0.019) and earlier (from the second semester of pregnancy) gestational age (<i>p</i>-value = 0.025) constituted the risk factors of maternal colonisation by GBS. In addition, the strains of GBS were resistant to all 16 antibiotics tested. A D test showed that 64.7% of GBS strains were constitutively resistant to clindamycin. We also note the presence of M phenotypes. As a whole, our results demonstrated that the rate of GBS colonisation in this study was similar to or higher than those in previous reports in Cameroon. All these results indicate that attention should be paid to this bacterium in the monitoring of antimicrobial resistance and in the care of pregnant women and newborns.https://www.mdpi.com/2036-7481/13/4/68antibiotic resistancecolonisationprevalenceGBSresistance phenotype |
| spellingShingle | Cécile Ingrid Djuikoue Paule Dana Djouela Djoulako Rodrigue Kamga Wouambo Rosine Yemetio Foutsa Dorine Ekeu Ngatcheu Teke Apalata Frequency and Antibiotic Susceptibility Patterns of <i>Streptococcus agalactiae</i> Strains Isolated from Women in Yaounde, Cameroon Microbiology Research antibiotic resistance colonisation prevalence GBS resistance phenotype |
| title | Frequency and Antibiotic Susceptibility Patterns of <i>Streptococcus agalactiae</i> Strains Isolated from Women in Yaounde, Cameroon |
| title_full | Frequency and Antibiotic Susceptibility Patterns of <i>Streptococcus agalactiae</i> Strains Isolated from Women in Yaounde, Cameroon |
| title_fullStr | Frequency and Antibiotic Susceptibility Patterns of <i>Streptococcus agalactiae</i> Strains Isolated from Women in Yaounde, Cameroon |
| title_full_unstemmed | Frequency and Antibiotic Susceptibility Patterns of <i>Streptococcus agalactiae</i> Strains Isolated from Women in Yaounde, Cameroon |
| title_short | Frequency and Antibiotic Susceptibility Patterns of <i>Streptococcus agalactiae</i> Strains Isolated from Women in Yaounde, Cameroon |
| title_sort | frequency and antibiotic susceptibility patterns of i streptococcus agalactiae i strains isolated from women in yaounde cameroon |
| topic | antibiotic resistance colonisation prevalence GBS resistance phenotype |
| url | https://www.mdpi.com/2036-7481/13/4/68 |
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