Temporal and Spatial Variation in the Population Structure of Spanish <i>Fusarium circinatum</i> Infecting Pine Stands
<i>Fusarium circinatum</i> is an introduced fungal pathogen extended to the northern regions of Spain that causes Pine Pitch Canker (PPC) disease. In this work, we analyzed the pathogen’s genetic diversity to study changes over time and space since the first outbreak occurred in Spain. U...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/2/159 |
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| author | David Fariña-Flores Mónica Berbegal Eugenia Iturritxa Laura Hernandez-Escribano Olga Aguín Rosa Raposo |
| author_facet | David Fariña-Flores Mónica Berbegal Eugenia Iturritxa Laura Hernandez-Escribano Olga Aguín Rosa Raposo |
| author_sort | David Fariña-Flores |
| collection | DOAJ |
| description | <i>Fusarium circinatum</i> is an introduced fungal pathogen extended to the northern regions of Spain that causes Pine Pitch Canker (PPC) disease. In this work, we analyzed the pathogen’s genetic diversity to study changes over time and space since the first outbreak occurred in Spain. Using six polymorphic SSR markers, 15 MLGs were identified in 66 isolates, and only three haplotypes were found with frequencies higher than one. In general, genotypic diversity was low and decreased shortly over time in the northwestern regions while maintained at País Vasco, where only one haplotype (MLG32) was detected 10 years. This population also included isolates of a single mating type (MAT-2) and VCGs identified in only two groups, while isolates from NW regions were of both mating types and VCGs represented in 11 groups. The existence of haplotype MLG32 maintained on time and widely distributed suggests its good adaptation to the environment and the host. Results showed that the pathogen in País Vasco remains clearly differentiated from other northwestern populations. This fact was supported with no evidence of migration among regions. Results are explained by the asexual reproduction, but also selfing at least to a lesser extent that leads to identification of two new haplotypes. |
| first_indexed | 2024-03-11T08:35:46Z |
| format | Article |
| id | doaj.art-80a363af254c48a48bc34f7f16cdf67d |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T08:35:46Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-80a363af254c48a48bc34f7f16cdf67d2023-11-16T21:29:54ZengMDPI AGJournal of Fungi2309-608X2023-01-019215910.3390/jof9020159Temporal and Spatial Variation in the Population Structure of Spanish <i>Fusarium circinatum</i> Infecting Pine StandsDavid Fariña-Flores0Mónica Berbegal1Eugenia Iturritxa2Laura Hernandez-Escribano3Olga Aguín4Rosa Raposo5Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, 28040 Madrid, SpainInstituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, SpainNEIKER, Granja Modelo—Arkaute, Apdo. 46, 01080 Vitoria-Gasteiz, SpainInstituto de Ciencias Forestales (ICIFOR-INIA), CSIC, Carretera Coruña km 7.5, 28040 Madrid, SpainEstación Fitopatolóxica Areeiro, Diputación Pontevedra, 36153 Pontevedra, SpainInstituto de Ciencias Forestales (ICIFOR-INIA), CSIC, Carretera Coruña km 7.5, 28040 Madrid, Spain<i>Fusarium circinatum</i> is an introduced fungal pathogen extended to the northern regions of Spain that causes Pine Pitch Canker (PPC) disease. In this work, we analyzed the pathogen’s genetic diversity to study changes over time and space since the first outbreak occurred in Spain. Using six polymorphic SSR markers, 15 MLGs were identified in 66 isolates, and only three haplotypes were found with frequencies higher than one. In general, genotypic diversity was low and decreased shortly over time in the northwestern regions while maintained at País Vasco, where only one haplotype (MLG32) was detected 10 years. This population also included isolates of a single mating type (MAT-2) and VCGs identified in only two groups, while isolates from NW regions were of both mating types and VCGs represented in 11 groups. The existence of haplotype MLG32 maintained on time and widely distributed suggests its good adaptation to the environment and the host. Results showed that the pathogen in País Vasco remains clearly differentiated from other northwestern populations. This fact was supported with no evidence of migration among regions. Results are explained by the asexual reproduction, but also selfing at least to a lesser extent that leads to identification of two new haplotypes.https://www.mdpi.com/2309-608X/9/2/159population genetic structurepine pitch canker diseasevegetative compatibility groupsmicrosatellite markersmating type |
| spellingShingle | David Fariña-Flores Mónica Berbegal Eugenia Iturritxa Laura Hernandez-Escribano Olga Aguín Rosa Raposo Temporal and Spatial Variation in the Population Structure of Spanish <i>Fusarium circinatum</i> Infecting Pine Stands Journal of Fungi population genetic structure pine pitch canker disease vegetative compatibility groups microsatellite markers mating type |
| title | Temporal and Spatial Variation in the Population Structure of Spanish <i>Fusarium circinatum</i> Infecting Pine Stands |
| title_full | Temporal and Spatial Variation in the Population Structure of Spanish <i>Fusarium circinatum</i> Infecting Pine Stands |
| title_fullStr | Temporal and Spatial Variation in the Population Structure of Spanish <i>Fusarium circinatum</i> Infecting Pine Stands |
| title_full_unstemmed | Temporal and Spatial Variation in the Population Structure of Spanish <i>Fusarium circinatum</i> Infecting Pine Stands |
| title_short | Temporal and Spatial Variation in the Population Structure of Spanish <i>Fusarium circinatum</i> Infecting Pine Stands |
| title_sort | temporal and spatial variation in the population structure of spanish i fusarium circinatum i infecting pine stands |
| topic | population genetic structure pine pitch canker disease vegetative compatibility groups microsatellite markers mating type |
| url | https://www.mdpi.com/2309-608X/9/2/159 |
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