Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs
Arene–ruthenium(II) complexes with carbothioamidopyrazoles at the C-2 and C-5 positions have been recognized as chemotherapeutic agent alternatives to cisplatin and its oxaliplatin analogs. The aim of this study was to continue research on the biological aspect of arene–ruthenium(II) complexes and t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/9/3969 |
| _version_ | 1797602105710608384 |
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| author | Paweł Hikisz Ewelina Namiecińska Piotr Paneth Elzbieta Budzisz |
| author_facet | Paweł Hikisz Ewelina Namiecińska Piotr Paneth Elzbieta Budzisz |
| author_sort | Paweł Hikisz |
| collection | DOAJ |
| description | Arene–ruthenium(II) complexes with carbothioamidopyrazoles at the C-2 and C-5 positions have been recognized as chemotherapeutic agent alternatives to cisplatin and its oxaliplatin analogs. The aim of this study was to continue research on the biological aspect of arene–ruthenium(II) complexes and their anticancer activity. The present paper includes an additional 12 new tumor cells, analyzed by MTT, and employs a series of extended bioassays to better understand their potential mechanism of antitumor activity. The following tests were conducted: membrane permeability studies, intramolecular reactive oxygen and nitrogen species (ROS/RNS) assays, mitochondrial potential changes, DNA analysis by comet assay using the electrophoresis method, measurement of cleaved PARP protein levels, and determination of apoptotic and necrotic cell fractions by fluorescence microscopy. Additionally, the article presents lipophilicity studies based on RP-TLC and molecular docking studies. We hope that the presented data will prove useful in practical treatment, especially for patients with cancer. |
| first_indexed | 2024-03-11T04:11:13Z |
| format | Article |
| id | doaj.art-80a587cc08654d6c8c5bc155f2efb501 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:11:13Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-80a587cc08654d6c8c5bc155f2efb5012023-11-17T23:25:53ZengMDPI AGMolecules1420-30492023-05-01289396910.3390/molecules28093969Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer DrugsPaweł Hikisz0Ewelina Namiecińska1Piotr Paneth2Elzbieta Budzisz3Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, PolandDepartment of the Chemistry of Cosmetic Raw Materials, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, PolandInstitute of Applied Radiation Chemistry, Department of Chemistry, Technical University of Lodz, Zeromskiego 116, 90-924 Lodz, PolandDepartment of the Chemistry of Cosmetic Raw Materials, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, PolandArene–ruthenium(II) complexes with carbothioamidopyrazoles at the C-2 and C-5 positions have been recognized as chemotherapeutic agent alternatives to cisplatin and its oxaliplatin analogs. The aim of this study was to continue research on the biological aspect of arene–ruthenium(II) complexes and their anticancer activity. The present paper includes an additional 12 new tumor cells, analyzed by MTT, and employs a series of extended bioassays to better understand their potential mechanism of antitumor activity. The following tests were conducted: membrane permeability studies, intramolecular reactive oxygen and nitrogen species (ROS/RNS) assays, mitochondrial potential changes, DNA analysis by comet assay using the electrophoresis method, measurement of cleaved PARP protein levels, and determination of apoptotic and necrotic cell fractions by fluorescence microscopy. Additionally, the article presents lipophilicity studies based on RP-TLC and molecular docking studies. We hope that the presented data will prove useful in practical treatment, especially for patients with cancer.https://www.mdpi.com/1420-3049/28/9/3969anticancer activitymolecular dockingarene–ruthenium(II) complexescarbothioamidopyrazoles |
| spellingShingle | Paweł Hikisz Ewelina Namiecińska Piotr Paneth Elzbieta Budzisz Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs Molecules anticancer activity molecular docking arene–ruthenium(II) complexes carbothioamidopyrazoles |
| title | Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs |
| title_full | Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs |
| title_fullStr | Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs |
| title_full_unstemmed | Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs |
| title_short | Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs |
| title_sort | mechanistic studies of arene ruthenium ii complexes with carbothioamidopyrazoles as alternative cancer drugs |
| topic | anticancer activity molecular docking arene–ruthenium(II) complexes carbothioamidopyrazoles |
| url | https://www.mdpi.com/1420-3049/28/9/3969 |
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