Chemical Biology Strategies to Study Autophagy
Growing amount of evidence in the last two decades highlight that macroautophagy (generally referred to as autophagy) is not only indispensable for survival in yeast but also equally important to maintain cellular quality control in higher eukaryotes as well. Importantly, dysfunctional autophagy has...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2018.00160/full |
| _version_ | 1818150463687622656 |
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| author | Piyush Mishra Veena Ammanathan Ravi Manjithaya |
| author_facet | Piyush Mishra Veena Ammanathan Ravi Manjithaya |
| author_sort | Piyush Mishra |
| collection | DOAJ |
| description | Growing amount of evidence in the last two decades highlight that macroautophagy (generally referred to as autophagy) is not only indispensable for survival in yeast but also equally important to maintain cellular quality control in higher eukaryotes as well. Importantly, dysfunctional autophagy has been explicitly shown to be involved in various physiological and pathological conditions such as cell death, cancer, neurodegenerative, and other diseases. Therefore, modulation and regulation of the autophagy pathway has emerged as an alternative strategy for the treatment of various disease conditions in the recent years. Several studies have shown genetic or pharmacological modulation of autophagy to be effective in treating cancer, clearing intracellular aggregates and pathogens. Understanding and controlling the autophagic flux, either through a genetic or pharmacological approach is therefore a highly promising approach and of great scientific interest as spatiotemporal and cell-tissue-organ level autophagy regulation is not clearly understood. Indeed, chemical biology approaches that identify small molecule effectors of autophagy have thus a dual benefit: the modulators act as tools to study and understand the process of autophagy, and may also have therapeutic potential. In this review, we discuss different strategies that have appeared to screen and identify potent small molecule modulators of autophagy. |
| first_indexed | 2024-12-11T13:23:20Z |
| format | Article |
| id | doaj.art-80a74b4d2d0d47c196386be6ad88cc9e |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-11T13:23:20Z |
| publishDate | 2018-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-80a74b4d2d0d47c196386be6ad88cc9e2022-12-22T01:05:41ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2018-11-01610.3389/fcell.2018.00160416685Chemical Biology Strategies to Study AutophagyPiyush MishraVeena AmmanathanRavi ManjithayaGrowing amount of evidence in the last two decades highlight that macroautophagy (generally referred to as autophagy) is not only indispensable for survival in yeast but also equally important to maintain cellular quality control in higher eukaryotes as well. Importantly, dysfunctional autophagy has been explicitly shown to be involved in various physiological and pathological conditions such as cell death, cancer, neurodegenerative, and other diseases. Therefore, modulation and regulation of the autophagy pathway has emerged as an alternative strategy for the treatment of various disease conditions in the recent years. Several studies have shown genetic or pharmacological modulation of autophagy to be effective in treating cancer, clearing intracellular aggregates and pathogens. Understanding and controlling the autophagic flux, either through a genetic or pharmacological approach is therefore a highly promising approach and of great scientific interest as spatiotemporal and cell-tissue-organ level autophagy regulation is not clearly understood. Indeed, chemical biology approaches that identify small molecule effectors of autophagy have thus a dual benefit: the modulators act as tools to study and understand the process of autophagy, and may also have therapeutic potential. In this review, we discuss different strategies that have appeared to screen and identify potent small molecule modulators of autophagy.https://www.frontiersin.org/article/10.3389/fcell.2018.00160/fullautophagyhigh throughputchemical biologyluciferasesmall molecule screeningfluorescence microscopy |
| spellingShingle | Piyush Mishra Veena Ammanathan Ravi Manjithaya Chemical Biology Strategies to Study Autophagy Frontiers in Cell and Developmental Biology autophagy high throughput chemical biology luciferase small molecule screening fluorescence microscopy |
| title | Chemical Biology Strategies to Study Autophagy |
| title_full | Chemical Biology Strategies to Study Autophagy |
| title_fullStr | Chemical Biology Strategies to Study Autophagy |
| title_full_unstemmed | Chemical Biology Strategies to Study Autophagy |
| title_short | Chemical Biology Strategies to Study Autophagy |
| title_sort | chemical biology strategies to study autophagy |
| topic | autophagy high throughput chemical biology luciferase small molecule screening fluorescence microscopy |
| url | https://www.frontiersin.org/article/10.3389/fcell.2018.00160/full |
| work_keys_str_mv | AT piyushmishra chemicalbiologystrategiestostudyautophagy AT veenaammanathan chemicalbiologystrategiestostudyautophagy AT ravimanjithaya chemicalbiologystrategiestostudyautophagy |