Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416
Abstract Background The resistance of Streptococcus pneumoniae to macrolides is becoming an increasingly important issue and thus it is important to understand the genetics related to adaptation of this species to the widespread use of antibiotics in Europe. The 58 isolates of S. pneumoniae belongin...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | BMC Microbiology |
| Online Access: | http://link.springer.com/article/10.1186/s12866-020-01909-1 |
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| author | Petra Spanelova Vladislav Jakubu Lucia Malisova Martin Musilek Jana Kozakova Costas C. Papagiannitsis Ibrahim Bitar Jaroslav Hrabak Annalisa Pantosti Maria del Grosso Helena Zemlickova |
| author_facet | Petra Spanelova Vladislav Jakubu Lucia Malisova Martin Musilek Jana Kozakova Costas C. Papagiannitsis Ibrahim Bitar Jaroslav Hrabak Annalisa Pantosti Maria del Grosso Helena Zemlickova |
| author_sort | Petra Spanelova |
| collection | DOAJ |
| description | Abstract Background The resistance of Streptococcus pneumoniae to macrolides is becoming an increasingly important issue and thus it is important to understand the genetics related to adaptation of this species to the widespread use of antibiotics in Europe. The 58 isolates of S. pneumoniae belonging to sequence type (ST) 416 and serotype 19A and to several different phenotypes originated from Italy, Portugal and Czech Republic were thus sequenced on Illumina MiSeq. The aim of the study was to describe genetical origine of isolates, investigate their macrolide resistance and suggest reasons for spread of ST416 in the Czech Republic. Results Investigation of genes associated with serotype determined serotype switch between 15B and 19A serotypes and core genome multilocus sequence typing (cgMLST) confirmed the origine of concerned isolates in Netherlands15B-37 clone. Inspected genomes proved variability of genes associated with the macrolide resistance even within closely genetically relative isolates. Conclusions Participation of 19A/ST416 on the spread of Netherlands15B-37 is accompanied by serotype switch between 19A and 15B serotypes and with acquisition of genes involved in macrolide resistance to the clone that was originally macrolide susceptible. There is evident tendency to interchanging and modifications of these and surrounding genes, that could lead to accelerate spreading of this sequence type in regions with high macrolide consumption. |
| first_indexed | 2024-12-12T23:50:36Z |
| format | Article |
| id | doaj.art-80aa20553c2d44a59ba81b80e7c4d9c6 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-12T23:50:36Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-80aa20553c2d44a59ba81b80e7c4d9c62022-12-22T00:06:43ZengBMCBMC Microbiology1471-21802020-07-0120111010.1186/s12866-020-01909-1Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416Petra Spanelova0Vladislav Jakubu1Lucia Malisova2Martin Musilek3Jana Kozakova4Costas C. Papagiannitsis5Ibrahim Bitar6Jaroslav Hrabak7Annalisa Pantosti8Maria del Grosso9Helena Zemlickova10Centre for Epidemiology and Microbiology, National Institute of Public HealthCentre for Epidemiology and Microbiology, National Institute of Public HealthCentre for Epidemiology and Microbiology, National Institute of Public HealthCentre for Epidemiology and Microbiology, National Institute of Public HealthCentre for Epidemiology and Microbiology, National Institute of Public HealthFaculty of Medicine, Biomedical Center, Charles UniversityFaculty of Medicine, Biomedical Center, Charles UniversityFaculty of Medicine, Biomedical Center, Charles UniversityDepartment of Infectious Diseases, Istituto Superiore di SanitàDepartment of Infectious Diseases, Istituto Superiore di SanitàCentre for Epidemiology and Microbiology, National Institute of Public HealthAbstract Background The resistance of Streptococcus pneumoniae to macrolides is becoming an increasingly important issue and thus it is important to understand the genetics related to adaptation of this species to the widespread use of antibiotics in Europe. The 58 isolates of S. pneumoniae belonging to sequence type (ST) 416 and serotype 19A and to several different phenotypes originated from Italy, Portugal and Czech Republic were thus sequenced on Illumina MiSeq. The aim of the study was to describe genetical origine of isolates, investigate their macrolide resistance and suggest reasons for spread of ST416 in the Czech Republic. Results Investigation of genes associated with serotype determined serotype switch between 15B and 19A serotypes and core genome multilocus sequence typing (cgMLST) confirmed the origine of concerned isolates in Netherlands15B-37 clone. Inspected genomes proved variability of genes associated with the macrolide resistance even within closely genetically relative isolates. Conclusions Participation of 19A/ST416 on the spread of Netherlands15B-37 is accompanied by serotype switch between 19A and 15B serotypes and with acquisition of genes involved in macrolide resistance to the clone that was originally macrolide susceptible. There is evident tendency to interchanging and modifications of these and surrounding genes, that could lead to accelerate spreading of this sequence type in regions with high macrolide consumption.http://link.springer.com/article/10.1186/s12866-020-01909-1 |
| spellingShingle | Petra Spanelova Vladislav Jakubu Lucia Malisova Martin Musilek Jana Kozakova Costas C. Papagiannitsis Ibrahim Bitar Jaroslav Hrabak Annalisa Pantosti Maria del Grosso Helena Zemlickova Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416 BMC Microbiology |
| title | Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416 |
| title_full | Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416 |
| title_fullStr | Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416 |
| title_full_unstemmed | Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416 |
| title_short | Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416 |
| title_sort | whole genome sequencing of macrolide resistant streptococcus pneumoniae serotype 19a sequence type 416 |
| url | http://link.springer.com/article/10.1186/s12866-020-01909-1 |
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