Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil
Even though the epidemiology of tick-borne agents (TBA) in dogs has been extensively investigated around the world, the occurrence, vectors involved, and molecular identity of these agents in cats remains elusive in many regions. Among TBA, <i>Ehrlichia</i>, <i>Anaplasma</i>,...
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MDPI AG
2022-01-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/1/106 |
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| author | Marcos Rogério André Ana Cláudia Calchi Maria Eduarda Chiaradia Furquim Isabela de Andrade Paulo Vitor Cadina Arantes Lara Cristina de Melo Lopes Iuri Kauan Lins do Nascimento Demarchi Mayra Araguaia Pereira Figueiredo Cirilo Antonio de Paula Lima Rosangela Zacarias Machado |
| author_facet | Marcos Rogério André Ana Cláudia Calchi Maria Eduarda Chiaradia Furquim Isabela de Andrade Paulo Vitor Cadina Arantes Lara Cristina de Melo Lopes Iuri Kauan Lins do Nascimento Demarchi Mayra Araguaia Pereira Figueiredo Cirilo Antonio de Paula Lima Rosangela Zacarias Machado |
| author_sort | Marcos Rogério André |
| collection | DOAJ |
| description | Even though the epidemiology of tick-borne agents (TBA) in dogs has been extensively investigated around the world, the occurrence, vectors involved, and molecular identity of these agents in cats remains elusive in many regions. Among TBA, <i>Ehrlichia</i>, <i>Anaplasma</i>, <i>Babesia</i>, <i>Cytauxzoon</i>, and <i>Hepatozoon</i> are responsible for diseases with non-specific clinical signs in cats, making essential the use of molecular techniques for accurate diagnosis and proper treatment. The present work aimed to investigate the occurrence and molecular identity of tick-borne agents (<i>Ehrlichia</i>, <i>Anaplasma</i>, <i>Babesia</i>/<i>Theileria</i>, <i>Cytauxzoon</i>, and <i>Hepatozoon</i>) in cats from southeastern (states of São Paulo (SP) and Minas Gerais (MG)) and northern (state of Rondônia (RO)) Brazil. For this purpose, 390 blood samples were collected from domiciled cats in MG (<i>n</i> = 155), SP (<i>n</i> = 151), and RO(<i>n</i> = 84) states, submitted to DNA extraction and PCR assays for <i>Ehrlichia</i> spp. (<i>dsb</i> gene), <i>Anaplasma</i> spp. (<i>rrs</i> gene), piroplasmids (18S rRNA gene), and <i>Hepatozoon</i> spp. (18S rRNA gene), sequencing, and phylogenetic inferences. The overall positivity for <i>Anaplasma</i> spp., <i>Ehrlichia</i> spp., <i>Babesia/Theileria</i> spp., <i>Cytauxzoon</i> spp., and <i>Hepatozoon</i> spp. were 7.4% (12.3% (MG) and 6.6% (SP)), 2% (4.5% (MG) and 0.6% (SP)), 0.7% (0.6% (MG), 0.6% (SP) and 1.2% (RO)), 27.2% (41.9% (MG), 24.5% (SP) and 4.8% (RO), and 0%, respectively. The phylogenetic analysis grouped the obtained sequences with ‘<i>Candidatus</i> Anaplasma amazonensis’, <i>A. platys</i>, <i>B. vogeli</i>, and <i>Cytauxzoon</i> sp. previously detected in wild felids from Brazil. qPCR specific for <i>E. canis</i> based on the <i>dsb</i> gene confirmed the molecular identity of the detected ehrlichial agent. The present study expanded the list and geographical distribution of hemoparasites in cats. ‘<i>Candidatus</i> Anaplasma amazonensis’, recently detected in sloths from northern Brazil, was described for the first time in cats. This is the first report of piroplasmids infecting cats in northern Brazil. Coinfection by <i>Cytauxzoon</i> and other TBA (<i>Ehrlichia, Anaplasma</i>, and <i>B. vogeli</i>) reported in the present study raises the need for veterinary practitioners’ awareness of cats parasitized by multiple TBA. |
| first_indexed | 2024-03-10T00:45:12Z |
| format | Article |
| id | doaj.art-80b47274d13740ce8ce008c8c14fe6f5 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T00:45:12Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj.art-80b47274d13740ce8ce008c8c14fe6f52023-11-23T15:00:28ZengMDPI AGPathogens2076-08172022-01-0111110610.3390/pathogens11010106Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern BrazilMarcos Rogério André0Ana Cláudia Calchi1Maria Eduarda Chiaradia Furquim2Isabela de Andrade3Paulo Vitor Cadina Arantes4Lara Cristina de Melo Lopes5Iuri Kauan Lins do Nascimento Demarchi6Mayra Araguaia Pereira Figueiredo7Cirilo Antonio de Paula Lima8Rosangela Zacarias Machado9Laboratório de Imunoparasitologia, Departamento de Patologia, Reprodução e Saúde Única—Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, UNESP, Jaboticabal 14884-900, SP, BrazilLaboratório de Imunoparasitologia, Departamento de Patologia, Reprodução e Saúde Única—Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, UNESP, Jaboticabal 14884-900, SP, BrazilLaboratório de Imunoparasitologia, Departamento de Patologia, Reprodução e Saúde Única—Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, UNESP, Jaboticabal 14884-900, SP, BrazilLaboratório de Imunoparasitologia, Departamento de Patologia, Reprodução e Saúde Única—Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, UNESP, Jaboticabal 14884-900, SP, BrazilLaboratório de Imunoparasitologia, Departamento de Patologia, Reprodução e Saúde Única—Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, UNESP, Jaboticabal 14884-900, SP, BrazilLaboratório de Imunoparasitologia, Departamento de Patologia, Reprodução e Saúde Única—Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, UNESP, Jaboticabal 14884-900, SP, BrazilLaboratório de Parasitologia, Entomologia e Biologia Molecular Aplicada à Saúde Única, Universidade Federal de Rondônia, Rolim de Moura 76940-000, RO, BrazilLaboratório de Parasitologia, Entomologia e Biologia Molecular Aplicada à Saúde Única, Universidade Federal de Rondônia, Rolim de Moura 76940-000, RO, BrazilDepartamento de Cirurgia Veterinária, Universidade Federal de Uberlândia, Uberlândia 38400-902, MG, BrazilLaboratório de Imunoparasitologia, Departamento de Patologia, Reprodução e Saúde Única—Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, UNESP, Jaboticabal 14884-900, SP, BrazilEven though the epidemiology of tick-borne agents (TBA) in dogs has been extensively investigated around the world, the occurrence, vectors involved, and molecular identity of these agents in cats remains elusive in many regions. Among TBA, <i>Ehrlichia</i>, <i>Anaplasma</i>, <i>Babesia</i>, <i>Cytauxzoon</i>, and <i>Hepatozoon</i> are responsible for diseases with non-specific clinical signs in cats, making essential the use of molecular techniques for accurate diagnosis and proper treatment. The present work aimed to investigate the occurrence and molecular identity of tick-borne agents (<i>Ehrlichia</i>, <i>Anaplasma</i>, <i>Babesia</i>/<i>Theileria</i>, <i>Cytauxzoon</i>, and <i>Hepatozoon</i>) in cats from southeastern (states of São Paulo (SP) and Minas Gerais (MG)) and northern (state of Rondônia (RO)) Brazil. For this purpose, 390 blood samples were collected from domiciled cats in MG (<i>n</i> = 155), SP (<i>n</i> = 151), and RO(<i>n</i> = 84) states, submitted to DNA extraction and PCR assays for <i>Ehrlichia</i> spp. (<i>dsb</i> gene), <i>Anaplasma</i> spp. (<i>rrs</i> gene), piroplasmids (18S rRNA gene), and <i>Hepatozoon</i> spp. (18S rRNA gene), sequencing, and phylogenetic inferences. The overall positivity for <i>Anaplasma</i> spp., <i>Ehrlichia</i> spp., <i>Babesia/Theileria</i> spp., <i>Cytauxzoon</i> spp., and <i>Hepatozoon</i> spp. were 7.4% (12.3% (MG) and 6.6% (SP)), 2% (4.5% (MG) and 0.6% (SP)), 0.7% (0.6% (MG), 0.6% (SP) and 1.2% (RO)), 27.2% (41.9% (MG), 24.5% (SP) and 4.8% (RO), and 0%, respectively. The phylogenetic analysis grouped the obtained sequences with ‘<i>Candidatus</i> Anaplasma amazonensis’, <i>A. platys</i>, <i>B. vogeli</i>, and <i>Cytauxzoon</i> sp. previously detected in wild felids from Brazil. qPCR specific for <i>E. canis</i> based on the <i>dsb</i> gene confirmed the molecular identity of the detected ehrlichial agent. The present study expanded the list and geographical distribution of hemoparasites in cats. ‘<i>Candidatus</i> Anaplasma amazonensis’, recently detected in sloths from northern Brazil, was described for the first time in cats. This is the first report of piroplasmids infecting cats in northern Brazil. Coinfection by <i>Cytauxzoon</i> and other TBA (<i>Ehrlichia, Anaplasma</i>, and <i>B. vogeli</i>) reported in the present study raises the need for veterinary practitioners’ awareness of cats parasitized by multiple TBA.https://www.mdpi.com/2076-0817/11/1/106feline<i>Anaplasma</i><i>Ehrlichia</i><i>Babesia</i><i>Cytauxzoon</i><i>Hepatozoon</i> |
| spellingShingle | Marcos Rogério André Ana Cláudia Calchi Maria Eduarda Chiaradia Furquim Isabela de Andrade Paulo Vitor Cadina Arantes Lara Cristina de Melo Lopes Iuri Kauan Lins do Nascimento Demarchi Mayra Araguaia Pereira Figueiredo Cirilo Antonio de Paula Lima Rosangela Zacarias Machado Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil Pathogens feline <i>Anaplasma</i> <i>Ehrlichia</i> <i>Babesia</i> <i>Cytauxzoon</i> <i>Hepatozoon</i> |
| title | Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil |
| title_full | Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil |
| title_fullStr | Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil |
| title_full_unstemmed | Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil |
| title_short | Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil |
| title_sort | molecular detection of tick borne agents in cats from southeastern and northern brazil |
| topic | feline <i>Anaplasma</i> <i>Ehrlichia</i> <i>Babesia</i> <i>Cytauxzoon</i> <i>Hepatozoon</i> |
| url | https://www.mdpi.com/2076-0817/11/1/106 |
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