Corticotropin-releasing hormone stimulates expression of leptin, 11beta-HSD2 and syncytin-1 in primary human trophoblasts
<p>Abstract</p> <p>Background</p> <p>The placental syncytiotrophoblast is the major source of maternal plasma corticotropin-releasing hormone (CRH) in the second half of pregnancy. Placental CRH exerts multiple functions in the maternal organism: It induces the adrenal...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-09-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://www.rbej.com/content/10/1/80 |
| _version_ | 1818528446134878208 |
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| author | Fahlbusch Fabian B Ruebner Matthias Volkert Gudrun Offergeld Ramona Hartner Andrea Menendez-Castro Carlos Strick Reiner Rauh Manfred Rascher Wolfgang Dötsch Jörg |
| author_facet | Fahlbusch Fabian B Ruebner Matthias Volkert Gudrun Offergeld Ramona Hartner Andrea Menendez-Castro Carlos Strick Reiner Rauh Manfred Rascher Wolfgang Dötsch Jörg |
| author_sort | Fahlbusch Fabian B |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The placental syncytiotrophoblast is the major source of maternal plasma corticotropin-releasing hormone (CRH) in the second half of pregnancy. Placental CRH exerts multiple functions in the maternal organism: It induces the adrenal secretion of cortisol via the stimulation of adrenocorticotropic hormone, regulates the timing of birth via its actions in the myometrium and inhibits the invasion of extravillous trophoblast cells in vitro. However, the auto- and paracrine actions of CRH on the syncytiotrophoblast itself are unknown. Intrauterine growth restriction (IUGR) is accompanied by an increase in placental CRH, which could be of pathophysiological relevance for the dysregulation in syncytialisation seen in IUGR placentas.</p> <p>Methods</p> <p>We aimed to determine the effect of CRH on isolated primary trophoblastic cells in vitro. After CRH stimulation the trophoblast syncytialisation rate was monitored via syncytin-1 gene expression and beta-hCG (beta-human chorionic gonadotropine) ELISA in culture supernatant. The expression of the IUGR marker genes leptin and 11beta-hydroxysteroid dehydrogenase 2 (11beta-HSD2) was measured continuously over a period of 72 h. We hypothesized that CRH might attenuate syncytialisation, induce leptin, and reduce 11beta-HSD2 expression in primary villous trophoblasts, which are known features of IUGR.</p> <p>Results</p> <p>CRH did not influence the differentiation of isolated trophoblasts into functional syncytium as determined by beta-hCG secretion, albeit inducing syncytin-1 expression. Following syncytialisation, CRH treatment significantly increased leptin and 11beta-HSD2 expression, as well as leptin secretion into culture supernatant after 48 h.</p> <p>Conclusion</p> <p>The relevance of CRH for placental physiology is underlined by the present in vitro study. The induction of leptin and 11beta-HSD2 in the syncytiotrophoblast by CRH might promote fetal nutrient supply and placental corticosteroid metabolism in the phase before labour induction.</p> |
| first_indexed | 2024-12-11T06:49:52Z |
| format | Article |
| id | doaj.art-80b916ff6c214983810df90c40eb508d |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-11T06:49:52Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
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| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-80b916ff6c214983810df90c40eb508d2022-12-22T01:16:56ZengBMCReproductive Biology and Endocrinology1477-78272012-09-011018010.1186/1477-7827-10-80Corticotropin-releasing hormone stimulates expression of leptin, 11beta-HSD2 and syncytin-1 in primary human trophoblastsFahlbusch Fabian BRuebner MatthiasVolkert GudrunOffergeld RamonaHartner AndreaMenendez-Castro CarlosStrick ReinerRauh ManfredRascher WolfgangDötsch Jörg<p>Abstract</p> <p>Background</p> <p>The placental syncytiotrophoblast is the major source of maternal plasma corticotropin-releasing hormone (CRH) in the second half of pregnancy. Placental CRH exerts multiple functions in the maternal organism: It induces the adrenal secretion of cortisol via the stimulation of adrenocorticotropic hormone, regulates the timing of birth via its actions in the myometrium and inhibits the invasion of extravillous trophoblast cells in vitro. However, the auto- and paracrine actions of CRH on the syncytiotrophoblast itself are unknown. Intrauterine growth restriction (IUGR) is accompanied by an increase in placental CRH, which could be of pathophysiological relevance for the dysregulation in syncytialisation seen in IUGR placentas.</p> <p>Methods</p> <p>We aimed to determine the effect of CRH on isolated primary trophoblastic cells in vitro. After CRH stimulation the trophoblast syncytialisation rate was monitored via syncytin-1 gene expression and beta-hCG (beta-human chorionic gonadotropine) ELISA in culture supernatant. The expression of the IUGR marker genes leptin and 11beta-hydroxysteroid dehydrogenase 2 (11beta-HSD2) was measured continuously over a period of 72 h. We hypothesized that CRH might attenuate syncytialisation, induce leptin, and reduce 11beta-HSD2 expression in primary villous trophoblasts, which are known features of IUGR.</p> <p>Results</p> <p>CRH did not influence the differentiation of isolated trophoblasts into functional syncytium as determined by beta-hCG secretion, albeit inducing syncytin-1 expression. Following syncytialisation, CRH treatment significantly increased leptin and 11beta-HSD2 expression, as well as leptin secretion into culture supernatant after 48 h.</p> <p>Conclusion</p> <p>The relevance of CRH for placental physiology is underlined by the present in vitro study. The induction of leptin and 11beta-HSD2 in the syncytiotrophoblast by CRH might promote fetal nutrient supply and placental corticosteroid metabolism in the phase before labour induction.</p>http://www.rbej.com/content/10/1/80CRHleptin11beta-HSD2Syncytin-1TrophoblastSyncytiotrophoblastPlacenta |
| spellingShingle | Fahlbusch Fabian B Ruebner Matthias Volkert Gudrun Offergeld Ramona Hartner Andrea Menendez-Castro Carlos Strick Reiner Rauh Manfred Rascher Wolfgang Dötsch Jörg Corticotropin-releasing hormone stimulates expression of leptin, 11beta-HSD2 and syncytin-1 in primary human trophoblasts Reproductive Biology and Endocrinology CRH leptin 11beta-HSD2 Syncytin-1 Trophoblast Syncytiotrophoblast Placenta |
| title | Corticotropin-releasing hormone stimulates expression of leptin, 11beta-HSD2 and syncytin-1 in primary human trophoblasts |
| title_full | Corticotropin-releasing hormone stimulates expression of leptin, 11beta-HSD2 and syncytin-1 in primary human trophoblasts |
| title_fullStr | Corticotropin-releasing hormone stimulates expression of leptin, 11beta-HSD2 and syncytin-1 in primary human trophoblasts |
| title_full_unstemmed | Corticotropin-releasing hormone stimulates expression of leptin, 11beta-HSD2 and syncytin-1 in primary human trophoblasts |
| title_short | Corticotropin-releasing hormone stimulates expression of leptin, 11beta-HSD2 and syncytin-1 in primary human trophoblasts |
| title_sort | corticotropin releasing hormone stimulates expression of leptin 11beta hsd2 and syncytin 1 in primary human trophoblasts |
| topic | CRH leptin 11beta-HSD2 Syncytin-1 Trophoblast Syncytiotrophoblast Placenta |
| url | http://www.rbej.com/content/10/1/80 |
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