Determination of the three forms of mercury in fish by L-cysteine extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry
ObjectiveThe aim of this study was to establish a method for determining inorganic， methyl， and ethyl mercury levels in fish using L-cysteine-assisted extraction-high-performance liquid chromatography-inductively coupled plasma mass spectrometry.MethodsL-cysteine was used to extract mercury from the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2023-10-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | https://www.zgspws.com/zgspwszz/article/abstract/202310008?st=article_issue |
| _version_ | 1797302285996392448 |
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| author | ZHONG Yang TAN Xin JIN Lixin HE Yexin QI Qihui RUAN Ruoyun |
| author_facet | ZHONG Yang TAN Xin JIN Lixin HE Yexin QI Qihui RUAN Ruoyun |
| author_sort | ZHONG Yang |
| collection | DOAJ |
| description | ObjectiveThe aim of this study was to establish a method for determining inorganic， methyl， and ethyl mercury levels in fish using L-cysteine-assisted extraction-high-performance liquid chromatography-inductively coupled plasma mass spectrometry.MethodsL-cysteine was used to extract mercury from the samples. The extraction efficiencies of different concentrations of L-cysteine were investigated. The effect of extraction time on extraction efficiency was also studied， and the mobile phase conditions were optimized.ResultsUsing a mobile phase comprising 2% methanol + 0.04 mol/L ammonium acetate + 1 g/L L-cysteine， the three forms of mercury in fish were determined by ultrasonic-assisted extraction with 1% L-cysteine for 10 min. All samples were separated within 9 min. The linear correlation coefficients for the three forms of mercury were over 0.999. The limits of detection of inorganic mercury， methyl mercury， and ethyl mercury were 3.0， 2.0， and 3.0 μg/kg， respectively. The spiked recoveries were 92.2%-97.9%， and the precision was less than 2%. The measured values of the quality control samples were within the range of the fixed values.ConclusionThe method developed herein is accurate， simple， convenient， and suitable for determining the three types of mercury in fish. |
| first_indexed | 2024-03-07T23:35:32Z |
| format | Article |
| id | doaj.art-80b93ba4060f4cda88317230c285c6a2 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-03-07T23:35:32Z |
| publishDate | 2023-10-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-80b93ba4060f4cda88317230c285c6a22024-02-20T07:51:14ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562023-10-0135101454145910.13590/j.cjfh.2023.10.0081004-8456（2023）10-1454-06Determination of the three forms of mercury in fish by L-cysteine extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometryZHONG YangTAN XinJIN LixinHE YexinQI QihuiRUAN RuoyunObjectiveThe aim of this study was to establish a method for determining inorganic， methyl， and ethyl mercury levels in fish using L-cysteine-assisted extraction-high-performance liquid chromatography-inductively coupled plasma mass spectrometry.MethodsL-cysteine was used to extract mercury from the samples. The extraction efficiencies of different concentrations of L-cysteine were investigated. The effect of extraction time on extraction efficiency was also studied， and the mobile phase conditions were optimized.ResultsUsing a mobile phase comprising 2% methanol + 0.04 mol/L ammonium acetate + 1 g/L L-cysteine， the three forms of mercury in fish were determined by ultrasonic-assisted extraction with 1% L-cysteine for 10 min. All samples were separated within 9 min. The linear correlation coefficients for the three forms of mercury were over 0.999. The limits of detection of inorganic mercury， methyl mercury， and ethyl mercury were 3.0， 2.0， and 3.0 μg/kg， respectively. The spiked recoveries were 92.2%-97.9%， and the precision was less than 2%. The measured values of the quality control samples were within the range of the fixed values.ConclusionThe method developed herein is accurate， simple， convenient， and suitable for determining the three types of mercury in fish.https://www.zgspws.com/zgspwszz/article/abstract/202310008?st=article_issuel-cysteinehigh-performance liquid chromatography-inductively coupled plasma mass spectrometryinorganic mercurymethyl mercuryethyl mercury |
| spellingShingle | ZHONG Yang TAN Xin JIN Lixin HE Yexin QI Qihui RUAN Ruoyun Determination of the three forms of mercury in fish by L-cysteine extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry Zhongguo shipin weisheng zazhi l-cysteine high-performance liquid chromatography-inductively coupled plasma mass spectrometry inorganic mercury methyl mercury ethyl mercury |
| title | Determination of the three forms of mercury in fish by L-cysteine extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry |
| title_full | Determination of the three forms of mercury in fish by L-cysteine extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry |
| title_fullStr | Determination of the three forms of mercury in fish by L-cysteine extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry |
| title_full_unstemmed | Determination of the three forms of mercury in fish by L-cysteine extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry |
| title_short | Determination of the three forms of mercury in fish by L-cysteine extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry |
| title_sort | determination of the three forms of mercury in fish by l cysteine extraction high performance liquid chromatography inductively coupled plasma mass spectrometry |
| topic | l-cysteine high-performance liquid chromatography-inductively coupled plasma mass spectrometry inorganic mercury methyl mercury ethyl mercury |
| url | https://www.zgspws.com/zgspwszz/article/abstract/202310008?st=article_issue |
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