Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers
Given the fatal health conditions caused by emerging infectious pathogens, such as severe acute respiratory syndrome coronavirus 2, their rapid diagnosis is required for preventing secondary infections and guiding correct treatments. Although various molecular diagnostic methods based on nucleic aci...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/11/4/125 |
| _version_ | 1797537531434106880 |
|---|---|
| author | Byungjun Park Bonhan Koo Jisub Kim Kiri Lee Hyeonjin Bang Sung-Han Kim Kyung Young Jhang Yong Shin Seungrag Lee |
| author_facet | Byungjun Park Bonhan Koo Jisub Kim Kiri Lee Hyeonjin Bang Sung-Han Kim Kyung Young Jhang Yong Shin Seungrag Lee |
| author_sort | Byungjun Park |
| collection | DOAJ |
| description | Given the fatal health conditions caused by emerging infectious pathogens, such as severe acute respiratory syndrome coronavirus 2, their rapid diagnosis is required for preventing secondary infections and guiding correct treatments. Although various molecular diagnostic methods based on nucleic acid amplification have been suggested as gold standards for identifying different species, these methods are not suitable for the rapid diagnosis of pathogens owing to their long result acquisition times and complexity. In this study, we developed a rapid bio-optical sensor that uses a ball-lensed optical fiber (BLOF) probe and an automatic analysis platform to precisely diagnose infectious pathogens. The BLOF probe is easy to align and has a high optical sensing sensitivity (1.5-fold) and a large detection range (1.2-fold) for an automatic optical sensing system. Automatic signal processing of up to 250 copies/reaction of DNA of Q-fever-causing <i>Coxiella burnetii</i> was achieved within 8 min. The clinical utility of this system was demonstrated with 18 clinical specimens (9 Q-fever and 9 other febrile disease samples) by measuring the resonant wavelength shift of positive or negative samples for <i>Coxiella burnetii</i> DNA. The results from the system revealed the stable and automatic optical signal measurement of DNA with 100% accuracy. We envision that this BLOF probe-based sensor would be a practical tool for the rapid, simple, and sensitive diagnosis of emerging infectious pathogens. |
| first_indexed | 2024-03-10T12:17:26Z |
| format | Article |
| id | doaj.art-80ba917e61f940a6accd2622e63120b6 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T12:17:26Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-80ba917e61f940a6accd2622e63120b62023-11-21T15:45:18ZengMDPI AGBiosensors2079-63742021-04-0111412510.3390/bios11040125Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical FibersByungjun Park0Bonhan Koo1Jisub Kim2Kiri Lee3Hyeonjin Bang4Sung-Han Kim5Kyung Young Jhang6Yong Shin7Seungrag Lee8Medical Device Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyung-ro, Heungdeok-gu, Cheongju-si 28160, KoreaDepartment of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, KoreaMedical Device Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyung-ro, Heungdeok-gu, Cheongju-si 28160, KoreaMedical Device Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyung-ro, Heungdeok-gu, Cheongju-si 28160, KoreaMedical Device Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyung-ro, Heungdeok-gu, Cheongju-si 28160, KoreaDepartment of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, KoreaSchool of Mechanical Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, KoreaDepartment of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei Ro, Seodaemun-gu, Seoul 03722, KoreaMedical Device Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyung-ro, Heungdeok-gu, Cheongju-si 28160, KoreaGiven the fatal health conditions caused by emerging infectious pathogens, such as severe acute respiratory syndrome coronavirus 2, their rapid diagnosis is required for preventing secondary infections and guiding correct treatments. Although various molecular diagnostic methods based on nucleic acid amplification have been suggested as gold standards for identifying different species, these methods are not suitable for the rapid diagnosis of pathogens owing to their long result acquisition times and complexity. In this study, we developed a rapid bio-optical sensor that uses a ball-lensed optical fiber (BLOF) probe and an automatic analysis platform to precisely diagnose infectious pathogens. The BLOF probe is easy to align and has a high optical sensing sensitivity (1.5-fold) and a large detection range (1.2-fold) for an automatic optical sensing system. Automatic signal processing of up to 250 copies/reaction of DNA of Q-fever-causing <i>Coxiella burnetii</i> was achieved within 8 min. The clinical utility of this system was demonstrated with 18 clinical specimens (9 Q-fever and 9 other febrile disease samples) by measuring the resonant wavelength shift of positive or negative samples for <i>Coxiella burnetii</i> DNA. The results from the system revealed the stable and automatic optical signal measurement of DNA with 100% accuracy. We envision that this BLOF probe-based sensor would be a practical tool for the rapid, simple, and sensitive diagnosis of emerging infectious pathogens.https://www.mdpi.com/2079-6374/11/4/125optical signal sensingfiber opticsemerging infectious pathogensrapid diagnosisQ fever |
| spellingShingle | Byungjun Park Bonhan Koo Jisub Kim Kiri Lee Hyeonjin Bang Sung-Han Kim Kyung Young Jhang Yong Shin Seungrag Lee Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers Biosensors optical signal sensing fiber optics emerging infectious pathogens rapid diagnosis Q fever |
| title | Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers |
| title_full | Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers |
| title_fullStr | Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers |
| title_full_unstemmed | Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers |
| title_short | Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers |
| title_sort | rapid molecular diagnostic sensor based on ball lensed optical fibers |
| topic | optical signal sensing fiber optics emerging infectious pathogens rapid diagnosis Q fever |
| url | https://www.mdpi.com/2079-6374/11/4/125 |
| work_keys_str_mv | AT byungjunpark rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers AT bonhankoo rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers AT jisubkim rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers AT kirilee rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers AT hyeonjinbang rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers AT sunghankim rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers AT kyungyoungjhang rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers AT yongshin rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers AT seungraglee rapidmoleculardiagnosticsensorbasedonballlensedopticalfibers |