CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains

CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains

Saccharomyces cerevisiae continues to serve as a powerful model system for both basic biological research and industrial application. The development of genome-wide collections of individually manipulated strains (libraries) has allowed for high-throughput genetic screens and an emerging global view...

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Bibliographic Details
Main Authors: Emily Roggenkamp, Rachael M. Giersch, Emily Wedeman, Muriel Eaton, Emily Turnquist, Madison N. Schrock, Linah Alkotami, Thitikan Jirakittisonthon, Samantha E. Schluter-Pascua, Gareth H. Bayne, Cory Wasko, Megan Halloran, Gregory C. Finnigan
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-09-01
Series:Frontiers in Microbiology
Subjects:
CRISPR
Cas9
budding yeast
libraries
marker-less integration
gene drive
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01773/full

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