Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of <i>Candida zeylanoides</i> Yeast Strain ATCC 20367
Used kitchen oil represents a feasible and renewable biomass to produce green biofuels such as biodiesel. Biodiesel production generates large amounts of by-products such as the crude glycerol fraction, which can be further used biotechnologically as a valuable nutrient for many microorganisms. In t...
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MDPI AG
2019-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/8/265 |
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| author | Laura Mitrea Floricuța Ranga Florinela Fetea Francisc Vasile Dulf Alexandru Rusu Monica Trif Dan Cristian Vodnar |
| author_facet | Laura Mitrea Floricuța Ranga Florinela Fetea Francisc Vasile Dulf Alexandru Rusu Monica Trif Dan Cristian Vodnar |
| author_sort | Laura Mitrea |
| collection | DOAJ |
| description | Used kitchen oil represents a feasible and renewable biomass to produce green biofuels such as biodiesel. Biodiesel production generates large amounts of by-products such as the crude glycerol fraction, which can be further used biotechnologically as a valuable nutrient for many microorganisms. In this study, we transesterified used kitchen oil with methanol and sodium hydroxide in order to obtain biodiesel and crude glycerol fractions. The crude glycerol fraction consisting of 30% glycerol was integrated into a bioreactor cultivation process as a nutrient source for the growth of <i>Candida zeylanoides</i> ATCC 20367. Cell viability and biomass production were similar to those obtained with batch cultivations on pure glycerol or glucose as the main nutrient substrates. However, the biosynthesis of organic acids (e.g., citric and succinic) was significantly different compared to pure glycerol and glucose used as main carbon sources. |
| first_indexed | 2024-12-13T12:54:18Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-13T12:54:18Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-80c1026ad196468db1a536c72b95b5bb2022-12-21T23:45:14ZengMDPI AGMicroorganisms2076-26072019-08-017826510.3390/microorganisms7080265microorganisms7080265Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of <i>Candida zeylanoides</i> Yeast Strain ATCC 20367Laura Mitrea0Floricuța Ranga1Florinela Fetea2Francisc Vasile Dulf3Alexandru Rusu4Monica Trif5Dan Cristian Vodnar6Institute of Life Sciences, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaInstitute of Life Sciences, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaInstitute of Life Sciences, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaFaculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaCENCIRA Agrofood Research and Innovation Centre, 6/66 Ion Meșter Street, 400650 Cluj-Napoca, RomaniaInstitute of Life Sciences, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaInstitute of Life Sciences, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaUsed kitchen oil represents a feasible and renewable biomass to produce green biofuels such as biodiesel. Biodiesel production generates large amounts of by-products such as the crude glycerol fraction, which can be further used biotechnologically as a valuable nutrient for many microorganisms. In this study, we transesterified used kitchen oil with methanol and sodium hydroxide in order to obtain biodiesel and crude glycerol fractions. The crude glycerol fraction consisting of 30% glycerol was integrated into a bioreactor cultivation process as a nutrient source for the growth of <i>Candida zeylanoides</i> ATCC 20367. Cell viability and biomass production were similar to those obtained with batch cultivations on pure glycerol or glucose as the main nutrient substrates. However, the biosynthesis of organic acids (e.g., citric and succinic) was significantly different compared to pure glycerol and glucose used as main carbon sources.https://www.mdpi.com/2076-2607/7/8/265renewable biomassused kitchen oilcrude glycerolorganic acids<i>Candida zeylanoides</i>fermentations |
| spellingShingle | Laura Mitrea Floricuța Ranga Florinela Fetea Francisc Vasile Dulf Alexandru Rusu Monica Trif Dan Cristian Vodnar Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of <i>Candida zeylanoides</i> Yeast Strain ATCC 20367 Microorganisms renewable biomass used kitchen oil crude glycerol organic acids <i>Candida zeylanoides</i> fermentations |
| title | Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of <i>Candida zeylanoides</i> Yeast Strain ATCC 20367 |
| title_full | Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of <i>Candida zeylanoides</i> Yeast Strain ATCC 20367 |
| title_fullStr | Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of <i>Candida zeylanoides</i> Yeast Strain ATCC 20367 |
| title_full_unstemmed | Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of <i>Candida zeylanoides</i> Yeast Strain ATCC 20367 |
| title_short | Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of <i>Candida zeylanoides</i> Yeast Strain ATCC 20367 |
| title_sort | biodiesel derived glycerol obtained from renewable biomass a suitable substrate for the growth of i candida zeylanoides i yeast strain atcc 20367 |
| topic | renewable biomass used kitchen oil crude glycerol organic acids <i>Candida zeylanoides</i> fermentations |
| url | https://www.mdpi.com/2076-2607/7/8/265 |
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