HIF-1α and GLUT-1 Expression in Atypical Endometrial Hyperplasia, Type I and II Endometrial Carcinoma: A Potential Role in Pathogenesis
Introduction: Hypoxia-Inducible Factor 1a (HIF-1α) is one of the major adaptive responses to hypoxia, regulating the activity of glucose transporter -1 (GLUT-1), responsible for glucose uptake. Aim: To evaluate the immunohistochemical expression of both HIF-1α and GLUT-1 in type I and II endomet...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/7805/19576_CE(RA1)_F(T)_PF1(ROAK)_PFA(AK)_PF2(PAG).pdf |
| Summary: | Introduction: Hypoxia-Inducible Factor 1a (HIF-1α) is one of the
major adaptive responses to hypoxia, regulating the activity of
glucose transporter -1 (GLUT-1), responsible for glucose uptake.
Aim: To evaluate the immunohistochemical expression of both
HIF-1α and GLUT-1 in type I and II endometrial carcinoma and
their correlation with the available clinicopathologic variables in
each type.
Materials and Methods: A retrospective study was conducted
on archival blocks diagnosed from pathology department
between April 2010 and August 2014 included 9 cases of
atypical hyperplasia and 67 cases of endometrial carcinoma.
Evaluation of both HIF-1α and GLUT-1 expression using
standard immunohistochemical techniques performed on cut
sections from selected paraffin embedded blocks.
Statistical Analysis: Descriptive analysis of the variables and
statistical significances were calculated by non-parametric chisquare test using the Statistical Package for the Social Sciences
version 12.0 (SPSS).
Results: HIF-1α was expressed in epithelial (88.9%, 52.2%,
61.2% and 50%) and stromal (33.3%, 74.6%. 71.4% and
83.3%) components of hyperplasia, total cases of EC, type I
and II EC, respectively. GLUT-1 was expressed in the epithelial
component of 88.9%, 98.5%, 98% and 100% of hyperplasia,
total EC cases, type I and II EC, respectively. The necrosis
related pattern of epithelial HIF-1α expression was in favour of
type II (p=0.018) and grade III (p=0.038). HIF-1α H-score was
associated with high apoptosis in both type I and total cases
of EC (p=0.04). GLUT-1 H-score was negatively correlated
with apoptotic count (p=0.04) and associated with high grade
(p=0.003) and advanced stage in total EC (p=0.004). GLUT-1
H-score was correlated with the pattern of HIF-1α staining in all
cases of EC (p= 0.04).
Conclusion: The role of HIF-1α in epithelial cells may differ
from that of stromal cells in EC; however they augment the
expression of each other supporting the crosstalk between
them. The stepwise increase in H- score of GLUT-1 in the studied
cases implies its potential role in carcinogenesis of EC. HIF-1α
may promote GLUT-1 expression in EC especially surrounding
areas of necrosis. The differences between type I and type II
EC regarding HIF-1α and GLUT-1 expression may confirm the
differences in their aetiopathogenesis. |
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| ISSN: | 2249-782X 0973-709X |