A Novel, Rapid Screening Technique for Sugar Syrup Adulteration in Honey Using Fluorescence Spectroscopy

The adulteration of honey with different sugar syrups is common and difficult to detect. To ensure fair trade and protect the interests of apiarists, a rapid, simple and cost-effective detection method for adulterants in honey is needed. In this work, fluorescence emission spectra were obtained for...

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Main Authors: Sha Yan, Minghui Sun, Xuan Wang, Jihao Shan, Xiaofeng Xue
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/11/15/2316
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author Sha Yan
Minghui Sun
Xuan Wang
Jihao Shan
Xiaofeng Xue
author_facet Sha Yan
Minghui Sun
Xuan Wang
Jihao Shan
Xiaofeng Xue
author_sort Sha Yan
collection DOAJ
description The adulteration of honey with different sugar syrups is common and difficult to detect. To ensure fair trade and protect the interests of apiarists, a rapid, simple and cost-effective detection method for adulterants in honey is needed. In this work, fluorescence emission spectra were obtained for honey and sugar syrups between 385 and 800 nm with excitation at 370 nm. We found substantial differences in the emission spectra between five types of honey and five sugar syrups and also found differences in their frequency doubled peak (FDP) intensity at 740 nm. The intensity of the FDP significantly declined (<i>p</i> < 0.01) when spiking honey with ≥10% sugar syrup. To validate this method, we tested 20 adulterant-positive honey samples and successfully identified 15 that were above the limit of detection. We propose that fluorescence spectroscopy could be broadly adopted as a cost-effective, rapid screening tool for sugar syrup adulteration of honey through characterization of emission spectra and the intensity of the FDP.
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spelling doaj.art-80c1972e9f3c4cbab6c21f87537638952023-12-01T22:56:18ZengMDPI AGFoods2304-81582022-08-011115231610.3390/foods11152316A Novel, Rapid Screening Technique for Sugar Syrup Adulteration in Honey Using Fluorescence SpectroscopySha Yan0Minghui Sun1Xuan Wang2Jihao Shan3Xiaofeng Xue4College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, ChinaInstitute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaThe adulteration of honey with different sugar syrups is common and difficult to detect. To ensure fair trade and protect the interests of apiarists, a rapid, simple and cost-effective detection method for adulterants in honey is needed. In this work, fluorescence emission spectra were obtained for honey and sugar syrups between 385 and 800 nm with excitation at 370 nm. We found substantial differences in the emission spectra between five types of honey and five sugar syrups and also found differences in their frequency doubled peak (FDP) intensity at 740 nm. The intensity of the FDP significantly declined (<i>p</i> < 0.01) when spiking honey with ≥10% sugar syrup. To validate this method, we tested 20 adulterant-positive honey samples and successfully identified 15 that were above the limit of detection. We propose that fluorescence spectroscopy could be broadly adopted as a cost-effective, rapid screening tool for sugar syrup adulteration of honey through characterization of emission spectra and the intensity of the FDP.https://www.mdpi.com/2304-8158/11/15/2316honeysugar syrupadulterationfluorescence spectroscopy
spellingShingle Sha Yan
Minghui Sun
Xuan Wang
Jihao Shan
Xiaofeng Xue
A Novel, Rapid Screening Technique for Sugar Syrup Adulteration in Honey Using Fluorescence Spectroscopy
Foods
honey
sugar syrup
adulteration
fluorescence spectroscopy
title A Novel, Rapid Screening Technique for Sugar Syrup Adulteration in Honey Using Fluorescence Spectroscopy
title_full A Novel, Rapid Screening Technique for Sugar Syrup Adulteration in Honey Using Fluorescence Spectroscopy
title_fullStr A Novel, Rapid Screening Technique for Sugar Syrup Adulteration in Honey Using Fluorescence Spectroscopy
title_full_unstemmed A Novel, Rapid Screening Technique for Sugar Syrup Adulteration in Honey Using Fluorescence Spectroscopy
title_short A Novel, Rapid Screening Technique for Sugar Syrup Adulteration in Honey Using Fluorescence Spectroscopy
title_sort novel rapid screening technique for sugar syrup adulteration in honey using fluorescence spectroscopy
topic honey
sugar syrup
adulteration
fluorescence spectroscopy
url https://www.mdpi.com/2304-8158/11/15/2316
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