Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity.
With the hope of understanding how interleukin (IL)-4 and IL-13 modulated quality of anti-viral CD8(+) T cells, we evaluated the expression of receptors for these cytokines following a range of viral infections (e.g. pox viruses and influenza virus). Results clearly indicated that unlike other IL-4/...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3561338?pdf=render |
| _version_ | 1811268728304697344 |
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| author | Danushka K Wijesundara David C Tscharke Ronald J Jackson Charani Ranasinghe |
| author_facet | Danushka K Wijesundara David C Tscharke Ronald J Jackson Charani Ranasinghe |
| author_sort | Danushka K Wijesundara |
| collection | DOAJ |
| description | With the hope of understanding how interleukin (IL)-4 and IL-13 modulated quality of anti-viral CD8(+) T cells, we evaluated the expression of receptors for these cytokines following a range of viral infections (e.g. pox viruses and influenza virus). Results clearly indicated that unlike other IL-4/IL-13 receptor subunits, IL-4 receptor α (IL-4Rα) was significantly down-regulated on anti-viral CD8(+) T cells in a cognate antigen dependent manner. The infection of gene knockout mice and wild-type (WT) mice with vaccinia virus (VV) or VV expressing IL-4 confirmed that IL-4, IL-13 and signal transducer and activator of transcription 6 (STAT6) were required to increase IL-4Rα expression on CD8(+) T cells, but not interferon (IFN)-γ. STAT6 dependent elevation of IL-4Rα expression on CD8(+) T cells was a feature of poor quality anti-viral CD8(+) T cell immunity as measured by the production of IFN-γ and tumor necrosis factor α (TNF-α) in response to VV antigen stimulation in vitro. We propose that down-regulation of IL-4Rα, but not the other IL-4/IL-13 receptor subunits, is a mechanism by which CD8(+) T cells reduce responsiveness to IL-4 and IL-13. This can improve the quality of anti-viral CD8(+) T cell immunity. Our findings have important implications in understanding anti-viral CD8(+) T cell immunity and designing effective vaccines against chronic viral infections. |
| first_indexed | 2024-04-12T21:28:06Z |
| format | Article |
| id | doaj.art-80c343fe4a3a4345bf62ebef52d3271a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T21:28:06Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-80c343fe4a3a4345bf62ebef52d3271a2022-12-22T03:16:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5578810.1371/journal.pone.0055788Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity.Danushka K WijesundaraDavid C TscharkeRonald J JacksonCharani RanasingheWith the hope of understanding how interleukin (IL)-4 and IL-13 modulated quality of anti-viral CD8(+) T cells, we evaluated the expression of receptors for these cytokines following a range of viral infections (e.g. pox viruses and influenza virus). Results clearly indicated that unlike other IL-4/IL-13 receptor subunits, IL-4 receptor α (IL-4Rα) was significantly down-regulated on anti-viral CD8(+) T cells in a cognate antigen dependent manner. The infection of gene knockout mice and wild-type (WT) mice with vaccinia virus (VV) or VV expressing IL-4 confirmed that IL-4, IL-13 and signal transducer and activator of transcription 6 (STAT6) were required to increase IL-4Rα expression on CD8(+) T cells, but not interferon (IFN)-γ. STAT6 dependent elevation of IL-4Rα expression on CD8(+) T cells was a feature of poor quality anti-viral CD8(+) T cell immunity as measured by the production of IFN-γ and tumor necrosis factor α (TNF-α) in response to VV antigen stimulation in vitro. We propose that down-regulation of IL-4Rα, but not the other IL-4/IL-13 receptor subunits, is a mechanism by which CD8(+) T cells reduce responsiveness to IL-4 and IL-13. This can improve the quality of anti-viral CD8(+) T cell immunity. Our findings have important implications in understanding anti-viral CD8(+) T cell immunity and designing effective vaccines against chronic viral infections.http://europepmc.org/articles/PMC3561338?pdf=render |
| spellingShingle | Danushka K Wijesundara David C Tscharke Ronald J Jackson Charani Ranasinghe Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity. PLoS ONE |
| title | Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity. |
| title_full | Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity. |
| title_fullStr | Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity. |
| title_full_unstemmed | Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity. |
| title_short | Reduced interleukin-4 receptor α expression on CD8+ T cells correlates with higher quality anti-viral immunity. |
| title_sort | reduced interleukin 4 receptor α expression on cd8 t cells correlates with higher quality anti viral immunity |
| url | http://europepmc.org/articles/PMC3561338?pdf=render |
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