Evaluation of blood PCR in the diagnosis of pulmonary tuberculosis
Background and Aim: Culture and specific staining (including Zeil-Nelson and fluorescent methods) are standard measures for the diagnosis of tuberculosis (TB).Because these methods are time-consuming and, sometimes, due to their low accuracy faster and more accurate methods are necessitated. Methods...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Birjand University of Medical Sciences and Health Services
2012-05-01
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| Series: | مجله دانشگاه علوم پزشکی بیرجند |
| Subjects: | |
| Online Access: | http://journal.bums.ac.ir/browse.php?a_code=A-10-363-2&slc_lang=en&sid=1 |
| _version_ | 1818553694914871296 |
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| author | Aliakbar Heydari Mohammadjavad Ghabooli Kiarash Ghazvini Maryam Mojtabavi |
| author_facet | Aliakbar Heydari Mohammadjavad Ghabooli Kiarash Ghazvini Maryam Mojtabavi |
| author_sort | Aliakbar Heydari |
| collection | DOAJ |
| description | Background and Aim: Culture and specific staining (including Zeil-Nelson and fluorescent methods) are standard measures for the diagnosis of tuberculosis (TB).Because these methods are time-consuming and, sometimes, due to their low accuracy faster and more accurate methods are necessitated. Methods, which can substitute invasive procedures, when obtaining smear samples and culture is not possible and in addition to being simple and fast, they have an acceptable diagnostic accuracy. The aim of the present study was to verify the diagnostic value of blood PCR in pulmonary TB. Materials and Methods: This case-control study included 64 proven pulmonary TB cases (according to The National TB Protocol) and 28 subjects who were completely healthy. 4.5ml of blood was derived from each participant and then mixed with 0.5ml EDTA. Finally, DNA extraction and PCR testing using SI 6110 primers was performed for all blood samples. Results: Mean age of the cases and controls was 49.8±18.6 and 48.2±18.5, respectively. 49.2% of the cases and 25% of controls were male. Blood PCR in 23 patients with TB was positive, but none of the controls had a positive PCR (thus, sensitivity of 35.7% and specificity of 100%). Conclusion: With regard to specificity of 100% in PCR method (despite its low sensitivity), in conditions where there is no access to an appropriate specimen, a positive blood PCR can obviate invasive procedures and rapid and definitive diagnosis of the disorder and timely treatment of the patient, his life is saved. |
| first_indexed | 2024-12-12T09:29:11Z |
| format | Article |
| id | doaj.art-80c462a75c51495bb57a0f4acac1848d |
| institution | Directory Open Access Journal |
| issn | 1607-2197 2423-6152 |
| language | fas |
| last_indexed | 2024-12-12T09:29:11Z |
| publishDate | 2012-05-01 |
| publisher | Birjand University of Medical Sciences and Health Services |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی بیرجند |
| spelling | doaj.art-80c462a75c51495bb57a0f4acac1848d2022-12-22T00:28:55ZfasBirjand University of Medical Sciences and Health Servicesمجله دانشگاه علوم پزشکی بیرجند1607-21972423-61522012-05-011913443Evaluation of blood PCR in the diagnosis of pulmonary tuberculosisAliakbar Heydari0Mohammadjavad Ghabooli1Kiarash Ghazvini2Maryam Mojtabavi3 Infectious Diseases Department, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran. Infectious Diseases Department, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran. Microbiology Research center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Taleghani Trauma Hospital, Mashhad University of Medical Sciences, Mashhad ,Iran. Background and Aim: Culture and specific staining (including Zeil-Nelson and fluorescent methods) are standard measures for the diagnosis of tuberculosis (TB).Because these methods are time-consuming and, sometimes, due to their low accuracy faster and more accurate methods are necessitated. Methods, which can substitute invasive procedures, when obtaining smear samples and culture is not possible and in addition to being simple and fast, they have an acceptable diagnostic accuracy. The aim of the present study was to verify the diagnostic value of blood PCR in pulmonary TB. Materials and Methods: This case-control study included 64 proven pulmonary TB cases (according to The National TB Protocol) and 28 subjects who were completely healthy. 4.5ml of blood was derived from each participant and then mixed with 0.5ml EDTA. Finally, DNA extraction and PCR testing using SI 6110 primers was performed for all blood samples. Results: Mean age of the cases and controls was 49.8±18.6 and 48.2±18.5, respectively. 49.2% of the cases and 25% of controls were male. Blood PCR in 23 patients with TB was positive, but none of the controls had a positive PCR (thus, sensitivity of 35.7% and specificity of 100%). Conclusion: With regard to specificity of 100% in PCR method (despite its low sensitivity), in conditions where there is no access to an appropriate specimen, a positive blood PCR can obviate invasive procedures and rapid and definitive diagnosis of the disorder and timely treatment of the patient, his life is saved.http://journal.bums.ac.ir/browse.php?a_code=A-10-363-2&slc_lang=en&sid=1Tuberculosis Mycobacterium tuberculosis PCR Sensitivity Specificity |
| spellingShingle | Aliakbar Heydari Mohammadjavad Ghabooli Kiarash Ghazvini Maryam Mojtabavi Evaluation of blood PCR in the diagnosis of pulmonary tuberculosis مجله دانشگاه علوم پزشکی بیرجند Tuberculosis Mycobacterium tuberculosis PCR Sensitivity Specificity |
| title | Evaluation of blood PCR in the diagnosis of pulmonary tuberculosis |
| title_full | Evaluation of blood PCR in the diagnosis of pulmonary tuberculosis |
| title_fullStr | Evaluation of blood PCR in the diagnosis of pulmonary tuberculosis |
| title_full_unstemmed | Evaluation of blood PCR in the diagnosis of pulmonary tuberculosis |
| title_short | Evaluation of blood PCR in the diagnosis of pulmonary tuberculosis |
| title_sort | evaluation of blood pcr in the diagnosis of pulmonary tuberculosis |
| topic | Tuberculosis Mycobacterium tuberculosis PCR Sensitivity Specificity |
| url | http://journal.bums.ac.ir/browse.php?a_code=A-10-363-2&slc_lang=en&sid=1 |
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