Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Isolated from Retail Meats in Korea
The spread of extended-spectrum beta-lactamase-producing <i>Escherichia coli</i> (ESBL-EC) has posed a critical health risk to both humans and animals, because resistance to beta-lactam antibiotics makes treatment for commonly infectious diseases more complicated. In this study, we repor...
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MDPI AG
2020-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/4/508 |
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| author | Seokhwan Kim Hansol Kim Yonghoon Kim Migyeong Kim Hyosun Kwak Sangryeol Ryu |
| author_facet | Seokhwan Kim Hansol Kim Yonghoon Kim Migyeong Kim Hyosun Kwak Sangryeol Ryu |
| author_sort | Seokhwan Kim |
| collection | DOAJ |
| description | The spread of extended-spectrum beta-lactamase-producing <i>Escherichia coli</i> (ESBL-EC) has posed a critical health risk to both humans and animals, because resistance to beta-lactam antibiotics makes treatment for commonly infectious diseases more complicated. In this study, we report the prevalence and genetic characteristics of ESBL-ECs isolated from retail meat samples in Korea. A total of 1205 <i>E. coli</i> strains were isolated from 3234 raw meat samples, purchased from nationwide retail stores between 2015 and 2018. Antimicrobial susceptibility testing was performed for all isolates by a broth microdilution method, and the ESBL phenotype was determined according to the Clinical and Laboratory Standards Institute (CLSI) confirmatory method. All ESBL-EC isolates (<i>n</i> = 29) were subjected to whole-genome sequencing (WGS). The antimicrobial resistance genes, plasmid incompatibility types, <i>E. coli</i> phylogroups, and phylogenetic relations were investigated based on the WGS data. The prevalence of ESBL-ECs in chicken was significantly higher than that in other meat samples. The results in this study demonstrate that clonally diverse ESBL-ECs with a multidrug resistance phenotype were distributed nationwide, although their prevalence from retail meat was 0.9%. The dissemination of ESBL-ECs from retail meat poses a potential risk to consumers and food-handlers, suggesting that the continuous surveillance of ESBL-ECs in retail meat should be conducted at the national level. |
| first_indexed | 2024-03-10T20:43:01Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:43:01Z |
| publishDate | 2020-04-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-80c4b0b0e4524c50a62714b8fac9320e2023-11-19T20:33:07ZengMDPI AGMicroorganisms2076-26072020-04-018450810.3390/microorganisms8040508Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Isolated from Retail Meats in KoreaSeokhwan Kim0Hansol Kim1Yonghoon Kim2Migyeong Kim3Hyosun Kwak4Sangryeol Ryu5Division of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, KoreaDivision of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, KoreaDivision of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, KoreaDivision of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, KoreaDivision of Food Microbiology, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, KoreaDepartment of Food and Animal Biotechnology, Seoul National University, Seoul 08826, KoreaThe spread of extended-spectrum beta-lactamase-producing <i>Escherichia coli</i> (ESBL-EC) has posed a critical health risk to both humans and animals, because resistance to beta-lactam antibiotics makes treatment for commonly infectious diseases more complicated. In this study, we report the prevalence and genetic characteristics of ESBL-ECs isolated from retail meat samples in Korea. A total of 1205 <i>E. coli</i> strains were isolated from 3234 raw meat samples, purchased from nationwide retail stores between 2015 and 2018. Antimicrobial susceptibility testing was performed for all isolates by a broth microdilution method, and the ESBL phenotype was determined according to the Clinical and Laboratory Standards Institute (CLSI) confirmatory method. All ESBL-EC isolates (<i>n</i> = 29) were subjected to whole-genome sequencing (WGS). The antimicrobial resistance genes, plasmid incompatibility types, <i>E. coli</i> phylogroups, and phylogenetic relations were investigated based on the WGS data. The prevalence of ESBL-ECs in chicken was significantly higher than that in other meat samples. The results in this study demonstrate that clonally diverse ESBL-ECs with a multidrug resistance phenotype were distributed nationwide, although their prevalence from retail meat was 0.9%. The dissemination of ESBL-ECs from retail meat poses a potential risk to consumers and food-handlers, suggesting that the continuous surveillance of ESBL-ECs in retail meat should be conducted at the national level.https://www.mdpi.com/2076-2607/8/4/508<i>Escherichia coli</i>extended-spectrum beta-lactamaseESBLretail meatwhole-genome sequencing |
| spellingShingle | Seokhwan Kim Hansol Kim Yonghoon Kim Migyeong Kim Hyosun Kwak Sangryeol Ryu Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Isolated from Retail Meats in Korea Microorganisms <i>Escherichia coli</i> extended-spectrum beta-lactamase ESBL retail meat whole-genome sequencing |
| title | Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Isolated from Retail Meats in Korea |
| title_full | Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Isolated from Retail Meats in Korea |
| title_fullStr | Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Isolated from Retail Meats in Korea |
| title_full_unstemmed | Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Isolated from Retail Meats in Korea |
| title_short | Whole-Genome Sequencing-Based Characteristics in Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> Isolated from Retail Meats in Korea |
| title_sort | whole genome sequencing based characteristics in extended spectrum beta lactamase producing i escherichia coli i isolated from retail meats in korea |
| topic | <i>Escherichia coli</i> extended-spectrum beta-lactamase ESBL retail meat whole-genome sequencing |
| url | https://www.mdpi.com/2076-2607/8/4/508 |
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