Osteogenic Potential of a Biomaterial Enriched with Osteostatin and Mesenchymal Stem Cells in Osteoporotic Rabbits
Mesoporous bioactive glasses (MBGs) of the SiO<sub>2</sub>–CaO–P<sub>2</sub>O<sub>5</sub> system are biocompatible materials with a quick and effective in vitro and in vivo bioactive response. MBGs can be enhanced by including therapeutically active ions in their...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/14/2/143 |
| _version_ | 1797298765964509184 |
|---|---|
| author | Gonzalo Luengo-Alonso Beatriz Bravo-Gimenez Daniel Lozano Clara Heras Sandra Sanchez-Salcedo Lorena Benito-Garzón Monica Abella María Vallet-Regi David Cecilia-Lopez Antonio J. Salinas |
| author_facet | Gonzalo Luengo-Alonso Beatriz Bravo-Gimenez Daniel Lozano Clara Heras Sandra Sanchez-Salcedo Lorena Benito-Garzón Monica Abella María Vallet-Regi David Cecilia-Lopez Antonio J. Salinas |
| author_sort | Gonzalo Luengo-Alonso |
| collection | DOAJ |
| description | Mesoporous bioactive glasses (MBGs) of the SiO<sub>2</sub>–CaO–P<sub>2</sub>O<sub>5</sub> system are biocompatible materials with a quick and effective in vitro and in vivo bioactive response. MBGs can be enhanced by including therapeutically active ions in their composition, by hosting osteogenic molecules within their mesopores, or by decorating their surfaces with mesenchymal stem cells (MSCs). In previous studies, our group showed that MBGs, ZnO-enriched and loaded with the osteogenic peptide osteostatin (OST), and MSCs exhibited osteogenic features under in vitro conditions. The aim of the present study was to evaluate bone repair capability after large bone defect treatment in distal femur osteoporotic rabbits using MBGs (76%SiO<sub>2</sub>–15%CaO–5%P<sub>2</sub>O<sub>5</sub>–4%ZnO (mol-%)) before and after loading with OST and MSCs from a donor rabbit. MSCs presence and/or OST in scaffolds significantly improved bone repair capacity at 6 and 12 weeks, as confirmed by variations observed in trabecular and cortical bone parameters obtained by micro-CT as well as histological analysis results. A greater effect was observed when OST and MSCs were combined. These findings may indicate the great potential for treating critical bone defects by combining MBGs with MSCs and osteogenic peptides such as OST, with good prospects for translation to clinical practice. |
| first_indexed | 2024-03-07T22:40:47Z |
| format | Article |
| id | doaj.art-80c6e880f5724df8b0d6de8f6675c5bd |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-07T22:40:47Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-80c6e880f5724df8b0d6de8f6675c5bd2024-02-23T15:09:12ZengMDPI AGBiomolecules2218-273X2024-01-0114214310.3390/biom14020143Osteogenic Potential of a Biomaterial Enriched with Osteostatin and Mesenchymal Stem Cells in Osteoporotic RabbitsGonzalo Luengo-Alonso0Beatriz Bravo-Gimenez1Daniel Lozano2Clara Heras3Sandra Sanchez-Salcedo4Lorena Benito-Garzón5Monica Abella6María Vallet-Regi7David Cecilia-Lopez8Antonio J. Salinas9Orthopaedics and Traumatology Service, Hospital Universitario Fundación Jiménez Díaz, 28040 Madrid, SpainOrthopedics and Traumatology Service, Hospital Universitario 12 de Octubre & Imas12, 28041 Madrid, SpainDepartment of Chemistry in Pharmaceutical Sciences, Universidad Complutense & Imas12, 28040 Madrid, SpainDepartment of Chemistry in Pharmaceutical Sciences, Universidad Complutense & Imas12, 28040 Madrid, SpainDepartment of Chemistry in Pharmaceutical Sciences, Universidad Complutense & Imas12, 28040 Madrid, SpainDepartment of Human Anatomy and Histology, Facultad de Medicina, Universidad de Salamanca, 37007 Salamanca, SpainDepartment of Bioengineering, Universidad Carlos III de Madrid, 28911 Madrid, SpainDepartment of Chemistry in Pharmaceutical Sciences, Universidad Complutense & Imas12, 28040 Madrid, SpainOrthopedics and Traumatology Service, Hospital Universitario 12 de Octubre & Imas12, 28041 Madrid, SpainDepartment of Chemistry in Pharmaceutical Sciences, Universidad Complutense & Imas12, 28040 Madrid, SpainMesoporous bioactive glasses (MBGs) of the SiO<sub>2</sub>–CaO–P<sub>2</sub>O<sub>5</sub> system are biocompatible materials with a quick and effective in vitro and in vivo bioactive response. MBGs can be enhanced by including therapeutically active ions in their composition, by hosting osteogenic molecules within their mesopores, or by decorating their surfaces with mesenchymal stem cells (MSCs). In previous studies, our group showed that MBGs, ZnO-enriched and loaded with the osteogenic peptide osteostatin (OST), and MSCs exhibited osteogenic features under in vitro conditions. The aim of the present study was to evaluate bone repair capability after large bone defect treatment in distal femur osteoporotic rabbits using MBGs (76%SiO<sub>2</sub>–15%CaO–5%P<sub>2</sub>O<sub>5</sub>–4%ZnO (mol-%)) before and after loading with OST and MSCs from a donor rabbit. MSCs presence and/or OST in scaffolds significantly improved bone repair capacity at 6 and 12 weeks, as confirmed by variations observed in trabecular and cortical bone parameters obtained by micro-CT as well as histological analysis results. A greater effect was observed when OST and MSCs were combined. These findings may indicate the great potential for treating critical bone defects by combining MBGs with MSCs and osteogenic peptides such as OST, with good prospects for translation to clinical practice.https://www.mdpi.com/2218-273X/14/2/143zinc-containing MBGsosteostatinmesenchymal stem cellsbone regenerationosteoporotic rabbits |
| spellingShingle | Gonzalo Luengo-Alonso Beatriz Bravo-Gimenez Daniel Lozano Clara Heras Sandra Sanchez-Salcedo Lorena Benito-Garzón Monica Abella María Vallet-Regi David Cecilia-Lopez Antonio J. Salinas Osteogenic Potential of a Biomaterial Enriched with Osteostatin and Mesenchymal Stem Cells in Osteoporotic Rabbits Biomolecules zinc-containing MBGs osteostatin mesenchymal stem cells bone regeneration osteoporotic rabbits |
| title | Osteogenic Potential of a Biomaterial Enriched with Osteostatin and Mesenchymal Stem Cells in Osteoporotic Rabbits |
| title_full | Osteogenic Potential of a Biomaterial Enriched with Osteostatin and Mesenchymal Stem Cells in Osteoporotic Rabbits |
| title_fullStr | Osteogenic Potential of a Biomaterial Enriched with Osteostatin and Mesenchymal Stem Cells in Osteoporotic Rabbits |
| title_full_unstemmed | Osteogenic Potential of a Biomaterial Enriched with Osteostatin and Mesenchymal Stem Cells in Osteoporotic Rabbits |
| title_short | Osteogenic Potential of a Biomaterial Enriched with Osteostatin and Mesenchymal Stem Cells in Osteoporotic Rabbits |
| title_sort | osteogenic potential of a biomaterial enriched with osteostatin and mesenchymal stem cells in osteoporotic rabbits |
| topic | zinc-containing MBGs osteostatin mesenchymal stem cells bone regeneration osteoporotic rabbits |
| url | https://www.mdpi.com/2218-273X/14/2/143 |
| work_keys_str_mv | AT gonzaloluengoalonso osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT beatrizbravogimenez osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT daniellozano osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT claraheras osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT sandrasanchezsalcedo osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT lorenabenitogarzon osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT monicaabella osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT mariavalletregi osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT davidcecilialopez osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits AT antoniojsalinas osteogenicpotentialofabiomaterialenrichedwithosteostatinandmesenchymalstemcellsinosteoporoticrabbits |