Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors.
Retinal OFF bipolar cells show distinct connectivity patterns with photoreceptors in the wild-type mouse retina. Some types are cone-specific while others penetrate further through the outer plexiform layer (OPL) to contact rods in addition to cones. To explore dendritic stratification of OFF bipola...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5336283?pdf=render |
| _version_ | 1819163767241441280 |
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| author | Christian Puller Patrick Arbogast Patrick W Keeley Benjamin E Reese Silke Haverkamp |
| author_facet | Christian Puller Patrick Arbogast Patrick W Keeley Benjamin E Reese Silke Haverkamp |
| author_sort | Christian Puller |
| collection | DOAJ |
| description | Retinal OFF bipolar cells show distinct connectivity patterns with photoreceptors in the wild-type mouse retina. Some types are cone-specific while others penetrate further through the outer plexiform layer (OPL) to contact rods in addition to cones. To explore dendritic stratification of OFF bipolar cells in the absence of rods, we made use of the 'cone-full' Nrl-/- mouse retina in which all photoreceptor precursor cells commit to a cone fate including those which would have become rods in wild-type retinas. The dendritic distribution of OFF bipolar cell types was investigated by confocal and electron microscopic imaging of immunolabeled tissue sections. The cells' dendrites formed basal contacts with cone terminals and expressed the corresponding glutamate receptor subunits at those sites, indicating putative synapses. All of the four analyzed cell populations showed distinctive patterns of vertical dendritic invasion through the OPL. This disparate behavior of dendritic extension in an environment containing only cone terminals demonstrates type-dependent specificity for dendritic outgrowth in OFF bipolar cells: rod terminals are not required for inducing dendritic extension into distal areas of the OPL. |
| first_indexed | 2024-12-22T17:49:22Z |
| format | Article |
| id | doaj.art-80c9645579df42baac3ebe479aed3ed6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T17:49:22Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-80c9645579df42baac3ebe479aed3ed62022-12-21T18:18:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017345510.1371/journal.pone.0173455Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors.Christian PullerPatrick ArbogastPatrick W KeeleyBenjamin E ReeseSilke HaverkampRetinal OFF bipolar cells show distinct connectivity patterns with photoreceptors in the wild-type mouse retina. Some types are cone-specific while others penetrate further through the outer plexiform layer (OPL) to contact rods in addition to cones. To explore dendritic stratification of OFF bipolar cells in the absence of rods, we made use of the 'cone-full' Nrl-/- mouse retina in which all photoreceptor precursor cells commit to a cone fate including those which would have become rods in wild-type retinas. The dendritic distribution of OFF bipolar cell types was investigated by confocal and electron microscopic imaging of immunolabeled tissue sections. The cells' dendrites formed basal contacts with cone terminals and expressed the corresponding glutamate receptor subunits at those sites, indicating putative synapses. All of the four analyzed cell populations showed distinctive patterns of vertical dendritic invasion through the OPL. This disparate behavior of dendritic extension in an environment containing only cone terminals demonstrates type-dependent specificity for dendritic outgrowth in OFF bipolar cells: rod terminals are not required for inducing dendritic extension into distal areas of the OPL.http://europepmc.org/articles/PMC5336283?pdf=render |
| spellingShingle | Christian Puller Patrick Arbogast Patrick W Keeley Benjamin E Reese Silke Haverkamp Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors. PLoS ONE |
| title | Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors. |
| title_full | Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors. |
| title_fullStr | Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors. |
| title_full_unstemmed | Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors. |
| title_short | Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors. |
| title_sort | dendritic stratification differs among retinal off bipolar cell types in the absence of rod photoreceptors |
| url | http://europepmc.org/articles/PMC5336283?pdf=render |
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