Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis
Interleukin (IL)-15 is a pleiotropic cytokine that regulates the proliferation and survival of many cell types. IL-15 is produced by monocytes and macrophages against infectious agents and plays a pivotal role in innate and adaptive immune responses. This study analyzed the effect of IL-15 on fungic...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-11-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000700005 |
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| author | Camila Ferreira Bannwart Rosana A Rodrigues Martins Érika Nakaira-Takahashi Luciane A Dias-Melício Ângela MV Campos Soares Maria Terezinha S Peraçoli |
| author_facet | Camila Ferreira Bannwart Rosana A Rodrigues Martins Érika Nakaira-Takahashi Luciane A Dias-Melício Ângela MV Campos Soares Maria Terezinha S Peraçoli |
| author_sort | Camila Ferreira Bannwart |
| collection | DOAJ |
| description | Interleukin (IL)-15 is a pleiotropic cytokine that regulates the proliferation and survival of many cell types. IL-15 is produced by monocytes and macrophages against infectious agents and plays a pivotal role in innate and adaptive immune responses. This study analyzed the effect of IL-15 on fungicidal activity, oxidative metabolism and cytokine production by human monocytes challenged in vitro with Paracoccidioides brasiliensis (Pb18), the agent of paracoccidioidomycosis. Peripheral blood monocytes were pre-incubated with IL-15 and then challenged with Pb18. Fungicidal activity was assessed by viable fungi recovery from cultures after plating on brain-heart infusion-agar. Superoxide anion (O2-), hydrogen peroxide (H2O2), tumour necrosis factor-alpha (TNF-α), IL-6, IL-15 and IL-10 production by monocytes were also determined. IL-15 enhanced fungicidal activity against Pb18 in a dose-dependent pattern. This effect was abrogated by addition of anti-IL-15 monoclonal antibody. A significant stimulatory effect of IL-15 on O2- and H2O2 release suggests that fungicidal activity was dependent on the activation of oxidative metabolism. Pre-treatment of monocytes with IL-15 induced significantly higher levels of TNF-α, IL-10 and IL-15 production by cells challenged with the fungus. These results suggest a modulatory effect of IL-15 on pro and anti-inflammatory cytokine production, oxidative metabolism and fungicidal activity of monocytes during Pb18 infection. |
| first_indexed | 2024-03-12T05:23:51Z |
| format | Article |
| id | doaj.art-80c9a1be46aa4e1bb9da45d349a3b8e1 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:23:51Z |
| publishDate | 2010-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-80c9a1be46aa4e1bb9da45d349a3b8e12023-09-03T07:27:14ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-11-01105786687210.1590/S0074-02762010000700005Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensisCamila Ferreira BannwartRosana A Rodrigues MartinsÉrika Nakaira-TakahashiLuciane A Dias-MelícioÂngela MV Campos SoaresMaria Terezinha S PeraçoliInterleukin (IL)-15 is a pleiotropic cytokine that regulates the proliferation and survival of many cell types. IL-15 is produced by monocytes and macrophages against infectious agents and plays a pivotal role in innate and adaptive immune responses. This study analyzed the effect of IL-15 on fungicidal activity, oxidative metabolism and cytokine production by human monocytes challenged in vitro with Paracoccidioides brasiliensis (Pb18), the agent of paracoccidioidomycosis. Peripheral blood monocytes were pre-incubated with IL-15 and then challenged with Pb18. Fungicidal activity was assessed by viable fungi recovery from cultures after plating on brain-heart infusion-agar. Superoxide anion (O2-), hydrogen peroxide (H2O2), tumour necrosis factor-alpha (TNF-α), IL-6, IL-15 and IL-10 production by monocytes were also determined. IL-15 enhanced fungicidal activity against Pb18 in a dose-dependent pattern. This effect was abrogated by addition of anti-IL-15 monoclonal antibody. A significant stimulatory effect of IL-15 on O2- and H2O2 release suggests that fungicidal activity was dependent on the activation of oxidative metabolism. Pre-treatment of monocytes with IL-15 induced significantly higher levels of TNF-α, IL-10 and IL-15 production by cells challenged with the fungus. These results suggest a modulatory effect of IL-15 on pro and anti-inflammatory cytokine production, oxidative metabolism and fungicidal activity of monocytes during Pb18 infection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000700005IL-15Paracoccidioides brasiliensismonocyteshydrogen peroxidefungicidal activitycytokines |
| spellingShingle | Camila Ferreira Bannwart Rosana A Rodrigues Martins Érika Nakaira-Takahashi Luciane A Dias-Melício Ângela MV Campos Soares Maria Terezinha S Peraçoli Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis Memorias do Instituto Oswaldo Cruz IL-15 Paracoccidioides brasiliensis monocytes hydrogen peroxide fungicidal activity cytokines |
| title | Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis |
| title_full | Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis |
| title_fullStr | Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis |
| title_full_unstemmed | Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis |
| title_short | Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis |
| title_sort | interleukin 15 augments oxidative metabolism and fungicidal activity of human monocytes against paracoccidioides brasiliensis |
| topic | IL-15 Paracoccidioides brasiliensis monocytes hydrogen peroxide fungicidal activity cytokines |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000700005 |
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