Comparison of self-collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among HIV infected women in Ethiopia
Abstract Background In order to meet the WHO 2030 cervical cancer elimination program, evaluation and utilization of sensitive testing method, and feasible sampling technique is a paradigm for enhancing cervical cancer screening coverage. Self-sampling for screening of HPV DNA testing is one of the...
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Women's Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12905-022-01944-2 |
| _version_ | 1811297501314023424 |
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| author | Agajie Likie Bogale Tilahun Teklehaymanot Jemal Haidar Ali Getnet Mitike Kassie Girmay Medhin Ajanaw Yizengaw Baye Amelework Yilma Shiferaw |
| author_facet | Agajie Likie Bogale Tilahun Teklehaymanot Jemal Haidar Ali Getnet Mitike Kassie Girmay Medhin Ajanaw Yizengaw Baye Amelework Yilma Shiferaw |
| author_sort | Agajie Likie Bogale |
| collection | DOAJ |
| description | Abstract Background In order to meet the WHO 2030 cervical cancer elimination program, evaluation and utilization of sensitive testing method, and feasible sampling technique is a paradigm for enhancing cervical cancer screening coverage. Self-sampling for screening of HPV DNA testing is one of the easiest and sensitive techniques, though the evidence was limited in the Ethiopian context. This study aimed to compare the performance of self-collected vaginal specimen versus clinician collected cervical specimen for detection of HPV among HIV positive women in Ethiopia. Methods We conducted a comparative cross-sectional study design to collect cervicovaginal specimens among HIV positive women of age older than 24 years. Data were collected from six government hospitals from January to October 2021. A total of 994 cervicovaginal specimens was collected by clinicians and HIV positive women themselves in the cervical cancer screening unit using Abbott Cervi-Collect Specimen Collection Kit, and molecular HPV testing was conducted. Data were entered into an Excel spreadsheet and analyzed using SPSS version 25. Sensitivity, specificity and kappa were reported with p < 0.05 considered as statistically significant. Results The prevalence of high-risk HPV was 29.4% among self-sampled specimen and 23.9% among clinician collected specimens. The overall concordance of the test result was 87.3%. Oncogenic HPV types, other than HPV16&18 were predominant in both sampling techniques, 19.9% from vaginal self-collected specimen and 16.7% of clinician collected cervical specimens. The sensitivity and specificity of self-sampled HPV test was 84.0% and 88.4%, respectively. The level of agreement was good (k = 0.68) and statistically significant (p < 0.001). The discriminatory power of the test as true positive and negative was excellent with an area under the curve of 0.86. Conclusion The magnitude of oncogenic HPV was higher in self-collected samples than the clinician collected specimen with good agreement between the two sampling methods. Thus, we recommend the Ministry of Health in Ethiopia to expand utilization of the self-sampled technique and enhance the coverage of screening in the country. |
| first_indexed | 2024-04-13T06:05:16Z |
| format | Article |
| id | doaj.art-80c9b45664114d57989cd99e06bf7d6e |
| institution | Directory Open Access Journal |
| issn | 1472-6874 |
| language | English |
| last_indexed | 2024-04-13T06:05:16Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
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| series | BMC Women's Health |
| spelling | doaj.art-80c9b45664114d57989cd99e06bf7d6e2022-12-22T02:59:17ZengBMCBMC Women's Health1472-68742022-09-012211710.1186/s12905-022-01944-2Comparison of self-collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among HIV infected women in EthiopiaAgajie Likie Bogale0Tilahun Teklehaymanot1Jemal Haidar Ali2Getnet Mitike Kassie3Girmay Medhin4Ajanaw Yizengaw Baye5Amelework Yilma Shiferaw6Program of Tropical and Infectious Diseases, Aklilu Lemma Institute of Pathobiology, Addis Ababa UniversityProgram of Tropical and Infectious Diseases, Aklilu Lemma Institute of Pathobiology, Addis Ababa UniversitySchool of Public Health, Addis Ababa UniversityInternational Institute of Primary Health CareProgram of Tropical and Infectious Diseases, Aklilu Lemma Institute of Pathobiology, Addis Ababa UniversityEthiopian Public Health InstituteEthiopian Public Health InstituteAbstract Background In order to meet the WHO 2030 cervical cancer elimination program, evaluation and utilization of sensitive testing method, and feasible sampling technique is a paradigm for enhancing cervical cancer screening coverage. Self-sampling for screening of HPV DNA testing is one of the easiest and sensitive techniques, though the evidence was limited in the Ethiopian context. This study aimed to compare the performance of self-collected vaginal specimen versus clinician collected cervical specimen for detection of HPV among HIV positive women in Ethiopia. Methods We conducted a comparative cross-sectional study design to collect cervicovaginal specimens among HIV positive women of age older than 24 years. Data were collected from six government hospitals from January to October 2021. A total of 994 cervicovaginal specimens was collected by clinicians and HIV positive women themselves in the cervical cancer screening unit using Abbott Cervi-Collect Specimen Collection Kit, and molecular HPV testing was conducted. Data were entered into an Excel spreadsheet and analyzed using SPSS version 25. Sensitivity, specificity and kappa were reported with p < 0.05 considered as statistically significant. Results The prevalence of high-risk HPV was 29.4% among self-sampled specimen and 23.9% among clinician collected specimens. The overall concordance of the test result was 87.3%. Oncogenic HPV types, other than HPV16&18 were predominant in both sampling techniques, 19.9% from vaginal self-collected specimen and 16.7% of clinician collected cervical specimens. The sensitivity and specificity of self-sampled HPV test was 84.0% and 88.4%, respectively. The level of agreement was good (k = 0.68) and statistically significant (p < 0.001). The discriminatory power of the test as true positive and negative was excellent with an area under the curve of 0.86. Conclusion The magnitude of oncogenic HPV was higher in self-collected samples than the clinician collected specimen with good agreement between the two sampling methods. Thus, we recommend the Ministry of Health in Ethiopia to expand utilization of the self-sampled technique and enhance the coverage of screening in the country.https://doi.org/10.1186/s12905-022-01944-2Cervical cancerCervicovaginal samplingHPV DNA testEthiopia |
| spellingShingle | Agajie Likie Bogale Tilahun Teklehaymanot Jemal Haidar Ali Getnet Mitike Kassie Girmay Medhin Ajanaw Yizengaw Baye Amelework Yilma Shiferaw Comparison of self-collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among HIV infected women in Ethiopia BMC Women's Health Cervical cancer Cervicovaginal sampling HPV DNA test Ethiopia |
| title | Comparison of self-collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among HIV infected women in Ethiopia |
| title_full | Comparison of self-collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among HIV infected women in Ethiopia |
| title_fullStr | Comparison of self-collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among HIV infected women in Ethiopia |
| title_full_unstemmed | Comparison of self-collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among HIV infected women in Ethiopia |
| title_short | Comparison of self-collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among HIV infected women in Ethiopia |
| title_sort | comparison of self collected versus clinician collected cervicovaginal specimens for detection of high risk human papillomavirus among hiv infected women in ethiopia |
| topic | Cervical cancer Cervicovaginal sampling HPV DNA test Ethiopia |
| url | https://doi.org/10.1186/s12905-022-01944-2 |
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