Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools
ABSTRACT Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas i...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academia Brasileira de Ciências
2016-01-01
|
| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200635&lng=en&tlng=en |
| _version_ | 1811243459103686656 |
|---|---|
| author | RODRIGO STAGGEMEIER THALITA ARANTES KARIN S. CAUMO MARILISE B. ROTT FERNANDO R. SPILKI |
| author_facet | RODRIGO STAGGEMEIER THALITA ARANTES KARIN S. CAUMO MARILISE B. ROTT FERNANDO R. SPILKI |
| author_sort | RODRIGO STAGGEMEIER |
| collection | DOAJ |
| description | ABSTRACT Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated from water samples collected from swimming pools in the city of Porto Alegre, Southern Brazil. Free-living amoebae of the genus Acanthamoeba were isolated from water samples, and isolates (n=16) were used to investigate the occurrence of HAdVs. HAdV detection was performed by quantitative real-time polymerase chain reaction (qPCR). HAdVs were detected in 62.5% (10/16) of Acanthamoeba isolates, ranging from 3.24x103 to 5.14x105 DNA copies per milliliter of isolate. HAdV viral loads found in this study are not negligible, especially because HAdV infections are associated with several human diseases, including gastroenteritis, respiratory distress, and ocular diseases. These findings reinforce the concept that Acanthamoeba may act as a reservoir and promote HAdV transmission through water. |
| first_indexed | 2024-04-12T14:07:42Z |
| format | Article |
| id | doaj.art-80cf6b2f6f764593b98c72c06ada4f8d |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-12T14:07:42Z |
| publishDate | 2016-01-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-80cf6b2f6f764593b98c72c06ada4f8d2022-12-22T03:30:01ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902016-01-0188suppl 163564110.1590/0001-3765201620150151S0001-37652016000200635Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming poolsRODRIGO STAGGEMEIERTHALITA ARANTESKARIN S. CAUMOMARILISE B. ROTTFERNANDO R. SPILKIABSTRACT Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated from water samples collected from swimming pools in the city of Porto Alegre, Southern Brazil. Free-living amoebae of the genus Acanthamoeba were isolated from water samples, and isolates (n=16) were used to investigate the occurrence of HAdVs. HAdV detection was performed by quantitative real-time polymerase chain reaction (qPCR). HAdVs were detected in 62.5% (10/16) of Acanthamoeba isolates, ranging from 3.24x103 to 5.14x105 DNA copies per milliliter of isolate. HAdV viral loads found in this study are not negligible, especially because HAdV infections are associated with several human diseases, including gastroenteritis, respiratory distress, and ocular diseases. These findings reinforce the concept that Acanthamoeba may act as a reservoir and promote HAdV transmission through water.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200635&lng=en&tlng=enAcanthamoebaameba de vida livreadenovírus humanoqPCRpiscinas |
| spellingShingle | RODRIGO STAGGEMEIER THALITA ARANTES KARIN S. CAUMO MARILISE B. ROTT FERNANDO R. SPILKI Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools Anais da Academia Brasileira de Ciências Acanthamoeba ameba de vida livre adenovírus humano qPCR piscinas |
| title | Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools |
| title_full | Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools |
| title_fullStr | Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools |
| title_full_unstemmed | Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools |
| title_short | Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools |
| title_sort | detection and quantification of human adenovirus genomes in acanthamoeba isolated from swimming pools |
| topic | Acanthamoeba ameba de vida livre adenovírus humano qPCR piscinas |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200635&lng=en&tlng=en |
| work_keys_str_mv | AT rodrigostaggemeier detectionandquantificationofhumanadenovirusgenomesinacanthamoebaisolatedfromswimmingpools AT thalitaarantes detectionandquantificationofhumanadenovirusgenomesinacanthamoebaisolatedfromswimmingpools AT karinscaumo detectionandquantificationofhumanadenovirusgenomesinacanthamoebaisolatedfromswimmingpools AT marilisebrott detectionandquantificationofhumanadenovirusgenomesinacanthamoebaisolatedfromswimmingpools AT fernandorspilki detectionandquantificationofhumanadenovirusgenomesinacanthamoebaisolatedfromswimmingpools |