Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 &...
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MDPI AG
2020-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/3/778 |
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| author | Ruben Daum Dmitri Visser Constanze Wild Larysa Kutuzova Maria Schneider Günter Lorenz Martin Weiss Svenja Hinderer Ulrich A. Stock Martina Seifert Katja Schenke-Layland |
| author_facet | Ruben Daum Dmitri Visser Constanze Wild Larysa Kutuzova Maria Schneider Günter Lorenz Martin Weiss Svenja Hinderer Ulrich A. Stock Martina Seifert Katja Schenke-Layland |
| author_sort | Ruben Daum |
| collection | DOAJ |
| description | Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer. |
| first_indexed | 2024-03-12T08:20:50Z |
| format | Article |
| id | doaj.art-80cfb0ee4ff04e5aabe1a04172b0bd8b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T08:20:50Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-80cfb0ee4ff04e5aabe1a04172b0bd8b2023-09-02T18:32:23ZengMDPI AGCells2073-44092020-03-019377810.3390/cells9030778cells9030778Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro CultureRuben Daum0Dmitri Visser1Constanze Wild2Larysa Kutuzova3Maria Schneider4Günter Lorenz5Martin Weiss6Svenja Hinderer7Ulrich A. Stock8Martina Seifert9Katja Schenke-Layland10NMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, GermanyNMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, GermanyInstitute of Medical Immunology and BIH Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 13353 Berlin, GermanyApplied Chemistry, University of Reutlingen, 72762 Reutlingen, GermanyInstitute of Medical Immunology and BIH Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 13353 Berlin, GermanyApplied Chemistry, University of Reutlingen, 72762 Reutlingen, GermanyNMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, GermanyNMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, GermanyDepartment of Cardiothoracic Surgery, Royal Brompton and Harefield Foundation Trust, Harefield Hospital Hill End Rd, Harefiled UB9 6JH, UKInstitute of Medical Immunology and BIH Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 13353 Berlin, GermanyNMI Natural and Medical Sciences Institute at the University of Tübingen, 72770 Reutlingen, GermanyAppropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer.https://www.mdpi.com/2073-4409/9/3/778vascular graftendothelializationtissue engineeringdecorinfibronectinelectrospinningendothelial progenitor cellsbioreactorbiostable polyurethane |
| spellingShingle | Ruben Daum Dmitri Visser Constanze Wild Larysa Kutuzova Maria Schneider Günter Lorenz Martin Weiss Svenja Hinderer Ulrich A. Stock Martina Seifert Katja Schenke-Layland Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture Cells vascular graft endothelialization tissue engineering decorin fibronectin electrospinning endothelial progenitor cells bioreactor biostable polyurethane |
| title | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
| title_full | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
| title_fullStr | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
| title_full_unstemmed | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
| title_short | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
| title_sort | fibronectin adsorption on electrospun synthetic vascular grafts attracts endothelial progenitor cells and promotes endothelialization in dynamic in vitro culture |
| topic | vascular graft endothelialization tissue engineering decorin fibronectin electrospinning endothelial progenitor cells bioreactor biostable polyurethane |
| url | https://www.mdpi.com/2073-4409/9/3/778 |
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