Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (diptera: culicidae) in Thailand
Anopheles members of the Barbirostris complex are important vectors of malaria in Thailand. However, they are morphologically indistinguishable because they are closely related species. In this study, wing geometric morphometrics (GM) and DNA barcoding based on the cytochrome c oxidase subunit 1 (CO...
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Elsevier
2022-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402202549X |
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| author | Tanawat Chaiphongpachara Tanasak Changbunjong Sedthapong Laojun Suchada Sumruayphol Nantana Suwandittakul Kewarin Kuntawong Siripong Pimsuka |
| author_facet | Tanawat Chaiphongpachara Tanasak Changbunjong Sedthapong Laojun Suchada Sumruayphol Nantana Suwandittakul Kewarin Kuntawong Siripong Pimsuka |
| author_sort | Tanawat Chaiphongpachara |
| collection | DOAJ |
| description | Anopheles members of the Barbirostris complex are important vectors of malaria in Thailand. However, they are morphologically indistinguishable because they are closely related species. In this study, wing geometric morphometrics (GM) and DNA barcoding based on the cytochrome c oxidase subunit 1 (COI) gene were applied to differentiate cryptic species of the Barbirostris complex in Thailand. Three cryptic species of the Barbirostris complex, Anopheles dissidens (19.44%), Anopheles saeungae (24.54%), and Anopheles wejchoochotei (56.02%) were initially identified using the multiplex polymerase chain reaction assay. DNA barcoding analyses showed low intraspecific distances (range, 0.27%–0.63%) and high interspecific distances (range, 1.92%–3.68%), consistent with the phylogenetic analyses that showed clear species groups. While wing size and shape analyses based on landmark-based GM indicated differences between three species (p < 0.05). The cross-validated reclassification revealed that the overall efficacy of wing size analysis for species identification of the Barbirostris complex was less than the wing shape analysis (56.43% vs. 74.29% total performance). Therefore, this study's results are guidelines for applying modern techniques to identify members within the Barbirostris complex, which are still difficult to distinguish by morphology-based identification and contribute to further appropriate malaria control. |
| first_indexed | 2024-04-12T17:51:54Z |
| format | Article |
| id | doaj.art-80cfd7853da74dbb98c3de500bd7e908 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T17:51:54Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-80cfd7853da74dbb98c3de500bd7e9082022-12-22T03:22:28ZengElsevierHeliyon2405-84402022-10-01810e11261Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (diptera: culicidae) in ThailandTanawat Chaiphongpachara0Tanasak Changbunjong1Sedthapong Laojun2Suchada Sumruayphol3Nantana Suwandittakul4Kewarin Kuntawong5Siripong Pimsuka6Department of Public Health and Health Promotion, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand; Corresponding author.Department of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand; The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MoZWE), Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, ThailandDepartment of Public Health and Health Promotion, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Bangkok 10300, ThailandDepartment of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Public Health and Health Promotion, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Bangkok 10300, ThailandDepartment of Public Health and Health Promotion, College of Allied Health Sciences, Suan Sunandha Rajabhat University, Bangkok 10300, ThailandSchool of Public Health, Eastern Asia University, Pathumthani 12110, ThailandAnopheles members of the Barbirostris complex are important vectors of malaria in Thailand. However, they are morphologically indistinguishable because they are closely related species. In this study, wing geometric morphometrics (GM) and DNA barcoding based on the cytochrome c oxidase subunit 1 (COI) gene were applied to differentiate cryptic species of the Barbirostris complex in Thailand. Three cryptic species of the Barbirostris complex, Anopheles dissidens (19.44%), Anopheles saeungae (24.54%), and Anopheles wejchoochotei (56.02%) were initially identified using the multiplex polymerase chain reaction assay. DNA barcoding analyses showed low intraspecific distances (range, 0.27%–0.63%) and high interspecific distances (range, 1.92%–3.68%), consistent with the phylogenetic analyses that showed clear species groups. While wing size and shape analyses based on landmark-based GM indicated differences between three species (p < 0.05). The cross-validated reclassification revealed that the overall efficacy of wing size analysis for species identification of the Barbirostris complex was less than the wing shape analysis (56.43% vs. 74.29% total performance). Therefore, this study's results are guidelines for applying modern techniques to identify members within the Barbirostris complex, which are still difficult to distinguish by morphology-based identification and contribute to further appropriate malaria control.http://www.sciencedirect.com/science/article/pii/S240584402202549XAnopheles barbirostris complexSpecies identificationDNA barcodingGeometric morphometrics |
| spellingShingle | Tanawat Chaiphongpachara Tanasak Changbunjong Sedthapong Laojun Suchada Sumruayphol Nantana Suwandittakul Kewarin Kuntawong Siripong Pimsuka Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (diptera: culicidae) in Thailand Heliyon Anopheles barbirostris complex Species identification DNA barcoding Geometric morphometrics |
| title | Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (diptera: culicidae) in Thailand |
| title_full | Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (diptera: culicidae) in Thailand |
| title_fullStr | Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (diptera: culicidae) in Thailand |
| title_full_unstemmed | Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (diptera: culicidae) in Thailand |
| title_short | Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (diptera: culicidae) in Thailand |
| title_sort | geometric morphometric and molecular techniques for discriminating among three cryptic species of the anopheles barbirostris complex diptera culicidae in thailand |
| topic | Anopheles barbirostris complex Species identification DNA barcoding Geometric morphometrics |
| url | http://www.sciencedirect.com/science/article/pii/S240584402202549X |
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