Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION)
Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the...
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| Format: | Article |
| Language: | English |
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Universitas Udayana
2016-01-01
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| Series: | Jurnal Veteriner |
| Subjects: | |
| Online Access: | https://ojs.unud.ac.id/index.php/jvet/article/view/17328 |
| _version_ | 1828451761265836032 |
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| author | Mazdani Ulfah Daulay Mirnawati Sudarwanto Widagdo Sri Nugroho Etih Sudarnika |
| author_facet | Mazdani Ulfah Daulay Mirnawati Sudarwanto Widagdo Sri Nugroho Etih Sudarnika |
| author_sort | Mazdani Ulfah Daulay |
| collection | DOAJ |
| description | Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Löwenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research. |
| first_indexed | 2024-12-10T23:42:34Z |
| format | Article |
| id | doaj.art-80d0c765ffa04c8e853e906c9086aaca |
| institution | Directory Open Access Journal |
| issn | 1411-8327 2477-5665 |
| language | English |
| last_indexed | 2024-12-10T23:42:34Z |
| publishDate | 2016-01-01 |
| publisher | Universitas Udayana |
| record_format | Article |
| series | Jurnal Veteriner |
| spelling | doaj.art-80d0c765ffa04c8e853e906c9086aaca2022-12-22T01:29:01ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652016-01-0116449750417328Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION)Mazdani Ulfah Daulay0Mirnawati SudarwantoWidagdo Sri NugrohoEtih SudarnikaFakultas Kedokteran Hewan, Universitas Udayana, BaliMycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Löwenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research.https://ojs.unud.ac.id/index.php/jvet/article/view/17328Mycobacterium bovismycobacterial cultureculture media |
| spellingShingle | Mazdani Ulfah Daulay Mirnawati Sudarwanto Widagdo Sri Nugroho Etih Sudarnika Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) Jurnal Veteriner Mycobacterium bovis mycobacterial culture culture media |
| title | Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) |
| title_full | Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) |
| title_fullStr | Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) |
| title_full_unstemmed | Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) |
| title_short | Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) |
| title_sort | pengembangan media padat untuk menumbuhkan mycobacterium bovis development of solid medium for mycobacterium bovis cultivation |
| topic | Mycobacterium bovis mycobacterial culture culture media |
| url | https://ojs.unud.ac.id/index.php/jvet/article/view/17328 |
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