Intestinal cholesterol absorption in the chyluria model
Isotopic methods for the measurement of dietary cholesterol absorption were compared with the lymph cholesterol balance procedure in filarial chyluria patients. After a single intravenous injection of radioactive cholesterol, absorption was found to be 746 +/- 136 mg/day by method I, which is based...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1987-10-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520386028 |
| _version_ | 1818590282450468864 |
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| author | S Q Doi H Meinertz K Nilausen E C Faria E C Quintão |
| author_facet | S Q Doi H Meinertz K Nilausen E C Faria E C Quintão |
| author_sort | S Q Doi |
| collection | DOAJ |
| description | Isotopic methods for the measurement of dietary cholesterol absorption were compared with the lymph cholesterol balance procedure in filarial chyluria patients. After a single intravenous injection of radioactive cholesterol, absorption was found to be 746 +/- 136 mg/day by method I, which is based upon the fecal endogenous neutral steroid mass measurement, and 471 +/- 135 mg/day by the simultaneously measured lymph/plasma ratio of cholesterol specific activity (dpm/mg). The corresponding value, determined as the difference between lymph cholesterol transport on a cholesterol-containing diet (1500 mg) and on a cholesterol-free diet, was 622 mg/day. When radioactive cholesterol (1487 mg/day) was fed daily to a second patient, absorption determined by isotopic fecal recovery (353 mg/day) matched that obtained by the lymph balance procedure (326 mg/day). Transudation of plasma cholesterol into the intestinal lymph, estimated by the single intravenous injection of radioactive beta-sitosterol, was independent of both the luminal content of plant sterols and the absorption of dietary cholesterol. The absorption of endogenous cholesterol was calculated by: 1) subtracting the cholesterol originating from plasma (transudation) together with the absorbed dietary cholesterol found in lymph from the total mass of cholesterol transported in lymph, and 2) the lymph balance method, i.e., after interrupting the endogenous cholesterol mucosal uptake by beta-sitosterol feeding (9 g/day) while on a cholesterol-free diet. Endogenous cholesterol was preferentially absorbed compared to dietary cholesterol, but there was no competition for absorption. The major portion of dietary cholesterol found in lymph was esterified, but esterification was not a prerequisite for absorption. |
| first_indexed | 2024-12-16T09:54:04Z |
| format | Article |
| id | doaj.art-80d3756a6b574979a6b33bef26269460 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T09:54:04Z |
| publishDate | 1987-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-80d3756a6b574979a6b33bef262694602022-12-21T22:35:58ZengElsevierJournal of Lipid Research0022-22751987-10-01281011291136Intestinal cholesterol absorption in the chyluria modelS Q Doi0H Meinertz1K Nilausen2E C Faria3E C Quintão4Department of Internal Medicine, University of São Paulo Medical School, Brazil.Department of Internal Medicine, University of São Paulo Medical School, Brazil.Department of Internal Medicine, University of São Paulo Medical School, Brazil.Department of Internal Medicine, University of São Paulo Medical School, Brazil.Department of Internal Medicine, University of São Paulo Medical School, Brazil.Isotopic methods for the measurement of dietary cholesterol absorption were compared with the lymph cholesterol balance procedure in filarial chyluria patients. After a single intravenous injection of radioactive cholesterol, absorption was found to be 746 +/- 136 mg/day by method I, which is based upon the fecal endogenous neutral steroid mass measurement, and 471 +/- 135 mg/day by the simultaneously measured lymph/plasma ratio of cholesterol specific activity (dpm/mg). The corresponding value, determined as the difference between lymph cholesterol transport on a cholesterol-containing diet (1500 mg) and on a cholesterol-free diet, was 622 mg/day. When radioactive cholesterol (1487 mg/day) was fed daily to a second patient, absorption determined by isotopic fecal recovery (353 mg/day) matched that obtained by the lymph balance procedure (326 mg/day). Transudation of plasma cholesterol into the intestinal lymph, estimated by the single intravenous injection of radioactive beta-sitosterol, was independent of both the luminal content of plant sterols and the absorption of dietary cholesterol. The absorption of endogenous cholesterol was calculated by: 1) subtracting the cholesterol originating from plasma (transudation) together with the absorbed dietary cholesterol found in lymph from the total mass of cholesterol transported in lymph, and 2) the lymph balance method, i.e., after interrupting the endogenous cholesterol mucosal uptake by beta-sitosterol feeding (9 g/day) while on a cholesterol-free diet. Endogenous cholesterol was preferentially absorbed compared to dietary cholesterol, but there was no competition for absorption. The major portion of dietary cholesterol found in lymph was esterified, but esterification was not a prerequisite for absorption.http://www.sciencedirect.com/science/article/pii/S0022227520386028 |
| spellingShingle | S Q Doi H Meinertz K Nilausen E C Faria E C Quintão Intestinal cholesterol absorption in the chyluria model Journal of Lipid Research |
| title | Intestinal cholesterol absorption in the chyluria model |
| title_full | Intestinal cholesterol absorption in the chyluria model |
| title_fullStr | Intestinal cholesterol absorption in the chyluria model |
| title_full_unstemmed | Intestinal cholesterol absorption in the chyluria model |
| title_short | Intestinal cholesterol absorption in the chyluria model |
| title_sort | intestinal cholesterol absorption in the chyluria model |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520386028 |
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