A Cytogenomic Analysis Reveals a New <i>Fusarium fujikuroi</i> Species Associated with Lemongrass (<i>Cymbopogon citratus</i>)
This study presents the first report of a <i>Fusarium</i> wilt in <i>Cymbopogon citratus</i> in Portugal. <i>Fusarium</i> is one of the most prominent genera of plant pathogens due to its wide range of hosts and mycotoxin production. The <i>Fusarium fujikuro...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2673-9976/27/1/21 |
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| author | Tomás Cavaco Catarina Gomes-Domingues Filipe Gonçalves |
| author_facet | Tomás Cavaco Catarina Gomes-Domingues Filipe Gonçalves |
| author_sort | Tomás Cavaco |
| collection | DOAJ |
| description | This study presents the first report of a <i>Fusarium</i> wilt in <i>Cymbopogon citratus</i> in Portugal. <i>Fusarium</i> is one of the most prominent genera of plant pathogens due to its wide range of hosts and mycotoxin production. The <i>Fusarium fujikuroi</i> species complex (FFSC) encompasses several threatening known plant pathogens. <i>Cymbopogon citratus</i> is a broadly distributed aromatic and medicinal plant rich in bioactive volatiles, which are relevant to several industries. The primary goal of this study was to identify and characterize the <i>Fusarium fujikuroi</i> species putatively responsible for the observed wilt. This report displays symptomatologic, cultural, morphologic, genetic, and cytogenomic characteristics associated with this fungus and disease. The cultural features included flat, white-colored colonies with filiform margins. Additionally, these colonies displayed abundant cottony aerial mycelia at the upper surface and orange-violet color at the lower surface. On Carnation Leaf-Piece Agar, septate fusoid macroconidia were present, displaying a flattened tapering toward the basal part and a number of septa ranging from one to four. The comparison between the amplified and aligned ITS sequences revealed 100% similarity between the isolate and the FFSC. Finally, a flow cytometry assay revealed an estimated genome size of 29.9 Mbp. This finding contrasts with other known pathogens from the <i>Fusarium fujikuroi</i> species complex. Ultimately, novel pathogens might be uncovered by exploring the mycobiome of diseased <i>Cymbopogon citratus</i> plants. |
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| language | English |
| last_indexed | 2024-04-24T18:30:10Z |
| publishDate | 2023-11-01 |
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| series | Biology and Life Sciences Forum |
| spelling | doaj.art-80d4230f683e4ff1b7ab870c6a6bb89c2024-03-27T13:28:23ZengMDPI AGBiology and Life Sciences Forum2673-99762023-11-012712110.3390/IECAG2023-15755A Cytogenomic Analysis Reveals a New <i>Fusarium fujikuroi</i> Species Associated with Lemongrass (<i>Cymbopogon citratus</i>)Tomás Cavaco0Catarina Gomes-Domingues1Filipe Gonçalves2Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-107 Lisboa, PortugalInstituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-107 Lisboa, PortugalInstituto Superior de Agronomia (ISA), Universidade de Lisboa, 1349-107 Lisboa, PortugalThis study presents the first report of a <i>Fusarium</i> wilt in <i>Cymbopogon citratus</i> in Portugal. <i>Fusarium</i> is one of the most prominent genera of plant pathogens due to its wide range of hosts and mycotoxin production. The <i>Fusarium fujikuroi</i> species complex (FFSC) encompasses several threatening known plant pathogens. <i>Cymbopogon citratus</i> is a broadly distributed aromatic and medicinal plant rich in bioactive volatiles, which are relevant to several industries. The primary goal of this study was to identify and characterize the <i>Fusarium fujikuroi</i> species putatively responsible for the observed wilt. This report displays symptomatologic, cultural, morphologic, genetic, and cytogenomic characteristics associated with this fungus and disease. The cultural features included flat, white-colored colonies with filiform margins. Additionally, these colonies displayed abundant cottony aerial mycelia at the upper surface and orange-violet color at the lower surface. On Carnation Leaf-Piece Agar, septate fusoid macroconidia were present, displaying a flattened tapering toward the basal part and a number of septa ranging from one to four. The comparison between the amplified and aligned ITS sequences revealed 100% similarity between the isolate and the FFSC. Finally, a flow cytometry assay revealed an estimated genome size of 29.9 Mbp. This finding contrasts with other known pathogens from the <i>Fusarium fujikuroi</i> species complex. Ultimately, novel pathogens might be uncovered by exploring the mycobiome of diseased <i>Cymbopogon citratus</i> plants.https://www.mdpi.com/2673-9976/27/1/21Cytogenomicslemongrassfungal pathogen<i>Fusarium fujikuroi</i> species complexplant pathology |
| spellingShingle | Tomás Cavaco Catarina Gomes-Domingues Filipe Gonçalves A Cytogenomic Analysis Reveals a New <i>Fusarium fujikuroi</i> Species Associated with Lemongrass (<i>Cymbopogon citratus</i>) Biology and Life Sciences Forum Cytogenomics lemongrass fungal pathogen <i>Fusarium fujikuroi</i> species complex plant pathology |
| title | A Cytogenomic Analysis Reveals a New <i>Fusarium fujikuroi</i> Species Associated with Lemongrass (<i>Cymbopogon citratus</i>) |
| title_full | A Cytogenomic Analysis Reveals a New <i>Fusarium fujikuroi</i> Species Associated with Lemongrass (<i>Cymbopogon citratus</i>) |
| title_fullStr | A Cytogenomic Analysis Reveals a New <i>Fusarium fujikuroi</i> Species Associated with Lemongrass (<i>Cymbopogon citratus</i>) |
| title_full_unstemmed | A Cytogenomic Analysis Reveals a New <i>Fusarium fujikuroi</i> Species Associated with Lemongrass (<i>Cymbopogon citratus</i>) |
| title_short | A Cytogenomic Analysis Reveals a New <i>Fusarium fujikuroi</i> Species Associated with Lemongrass (<i>Cymbopogon citratus</i>) |
| title_sort | cytogenomic analysis reveals a new i fusarium fujikuroi i species associated with lemongrass i cymbopogon citratus i |
| topic | Cytogenomics lemongrass fungal pathogen <i>Fusarium fujikuroi</i> species complex plant pathology |
| url | https://www.mdpi.com/2673-9976/27/1/21 |
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