Sequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA gene
Abstract Background Sarcocystis hirsuta, a tissue cyst-forming coccidian parasite of cattle, is worldwide in distribution. In spite of its global presence, limited literature is available on its characterization studies. No literature is available from India on molecular aspects of S. hirsuta. The p...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-03-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
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| Online Access: | https://doi.org/10.1186/s43088-021-00112-z |
| _version_ | 1818414811769208832 |
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| author | Vikrant Sudan Rahul Kumar Daya Shanker Amit Singh |
| author_facet | Vikrant Sudan Rahul Kumar Daya Shanker Amit Singh |
| author_sort | Vikrant Sudan |
| collection | DOAJ |
| description | Abstract Background Sarcocystis hirsuta, a tissue cyst-forming coccidian parasite of cattle, is worldwide in distribution. In spite of its global presence, limited literature is available on its characterization studies. No literature is available from India on molecular aspects of S. hirsuta. The present study was designed to characterize the isolates of S. hirsuta on the 18S gene locus. A total of five isolates of S. hirsuta were characterized. PCR products were cloned, sequenced, and compared with other sequences across the world. A phylogenetic tree was constructed based on the maximum parsimony (MP) method with the tree–bisection–regrafting (TBR) algorithm. Results An appreciable genetic variability was noticed between various S. hirsuta isolates at the 18S gene locus. Sequences generated from the present study (MN121567–MN121571) represented two haplotypes with 99.74–100.00% nucleotide homology within themselves. Alongside, a nucleotide homology of 97.82–99.92% was observed between Indian isolates and isolates across the globe. The two haplotypes were markedly distinct from each other with 3 nucleotide substitutions within themselves. Overall, Indian isolates of S. hirsuta were close to those from China and Vietnam than to those from New Zealand, Brazil, and Germany. Conclusion The present communication describes the first report of phylogenetic characterization of S. hirsuta from India. The findings are very much important in delineating the evolutionary phylogenetics of S. hirsuta. |
| first_indexed | 2024-12-14T11:25:02Z |
| format | Article |
| id | doaj.art-80d44f0280b040889343457927aa5135 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-12-14T11:25:02Z |
| publishDate | 2021-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-80d44f0280b040889343457927aa51352022-12-21T23:03:34ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432021-03-011011510.1186/s43088-021-00112-zSequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA geneVikrant Sudan0Rahul Kumar1Daya Shanker2Amit Singh3Department of Parasitology, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU)Department of Pathology, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU)Department of Parasitology, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU)Department of Parasitology, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU)Abstract Background Sarcocystis hirsuta, a tissue cyst-forming coccidian parasite of cattle, is worldwide in distribution. In spite of its global presence, limited literature is available on its characterization studies. No literature is available from India on molecular aspects of S. hirsuta. The present study was designed to characterize the isolates of S. hirsuta on the 18S gene locus. A total of five isolates of S. hirsuta were characterized. PCR products were cloned, sequenced, and compared with other sequences across the world. A phylogenetic tree was constructed based on the maximum parsimony (MP) method with the tree–bisection–regrafting (TBR) algorithm. Results An appreciable genetic variability was noticed between various S. hirsuta isolates at the 18S gene locus. Sequences generated from the present study (MN121567–MN121571) represented two haplotypes with 99.74–100.00% nucleotide homology within themselves. Alongside, a nucleotide homology of 97.82–99.92% was observed between Indian isolates and isolates across the globe. The two haplotypes were markedly distinct from each other with 3 nucleotide substitutions within themselves. Overall, Indian isolates of S. hirsuta were close to those from China and Vietnam than to those from New Zealand, Brazil, and Germany. Conclusion The present communication describes the first report of phylogenetic characterization of S. hirsuta from India. The findings are very much important in delineating the evolutionary phylogenetics of S. hirsuta.https://doi.org/10.1186/s43088-021-00112-z18SSarcocystis hirsutaPhylogenetic analysis |
| spellingShingle | Vikrant Sudan Rahul Kumar Daya Shanker Amit Singh Sequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA gene Beni-Suef University Journal of Basic and Applied Sciences 18S Sarcocystis hirsuta Phylogenetic analysis |
| title | Sequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA gene |
| title_full | Sequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA gene |
| title_fullStr | Sequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA gene |
| title_full_unstemmed | Sequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA gene |
| title_short | Sequence phylogenetic analysis and associative genetic diversity of Sarcocystis hirsuta based on 18S rRNA gene |
| title_sort | sequence phylogenetic analysis and associative genetic diversity of sarcocystis hirsuta based on 18s rrna gene |
| topic | 18S Sarcocystis hirsuta Phylogenetic analysis |
| url | https://doi.org/10.1186/s43088-021-00112-z |
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