Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus
<p>Infection of <em>Onion yellow dwarf virus </em>(OYDV) are reported causing problems in garlic production. Planting virus-free bulbs might help reduce viral disease incidence in the field. This research was aimed to develop method for eliminating OYDV from garlic bulbs using comb...
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| Format: | Article |
| Language: | Indonesian |
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Perhimpunan Fitopatologi Indonesia
2018-06-01
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| Series: | Jurnal Fitopatologi Indonesia |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/jfiti/article/view/21882 |
| _version_ | 1818138316971704320 |
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| author | Siti Shofiya Nasution Diny Dinarti Sri Hendrastuti Hidayat |
| author_facet | Siti Shofiya Nasution Diny Dinarti Sri Hendrastuti Hidayat |
| author_sort | Siti Shofiya Nasution |
| collection | DOAJ |
| description | <p>Infection of <em>Onion yellow dwarf virus </em>(OYDV) are reported causing problems in garlic production. Planting virus-free bulbs might help reduce viral disease incidence in the field. This research was aimed to develop method for eliminating OYDV from garlic bulbs using combination of electrotherapy (0, 5, 10, 15, and 20 mA each for 10 minutes) and thermotherapy (23, 28, 33, 38°C each for 4 weeks). Two garlic cultivars, i.e. Sangga Sembalun and Lumbu Hijau were used as seed bulbs for OYDV elimination tests. Virus infection was confirmed using <em>transcription-polymerase chain reaction</em> (RT-PCR). The result showed that thermotherapy at 33 °Cwas the best method to eliminate OYDV in garlic although the efficiency was not the same for all cultivars. The efficiency reached up to 60% for cv. Lumbu Hijau, whereas for cv. Sangga Sembalun only reached up to 40%. Electrotherapy alone or in combination with thermotherapy were not able to produce OYDV-free plantlets.</p> |
| first_indexed | 2024-12-11T10:10:16Z |
| format | Article |
| id | doaj.art-80d45d75b0864f6caa0e7c7dd93e3235 |
| institution | Directory Open Access Journal |
| issn | 0215-7950 2339-2479 |
| language | Indonesian |
| last_indexed | 2024-12-11T10:10:16Z |
| publishDate | 2018-06-01 |
| publisher | Perhimpunan Fitopatologi Indonesia |
| record_format | Article |
| series | Jurnal Fitopatologi Indonesia |
| spelling | doaj.art-80d45d75b0864f6caa0e7c7dd93e32352022-12-22T01:11:46ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792018-06-0113610.14692/jfi.13.6.19914147Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virusSiti Shofiya Nasution0Diny Dinarti1Sri Hendrastuti Hidayat2Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor, Jalan Kamper, Kampus Darmaga IPB, Bogor 16680Institut Pertanian Bogor, Bogor 16680Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor, Jalan Kamper, Kampus Darmaga IPB, Bogor 16680<p>Infection of <em>Onion yellow dwarf virus </em>(OYDV) are reported causing problems in garlic production. Planting virus-free bulbs might help reduce viral disease incidence in the field. This research was aimed to develop method for eliminating OYDV from garlic bulbs using combination of electrotherapy (0, 5, 10, 15, and 20 mA each for 10 minutes) and thermotherapy (23, 28, 33, 38°C each for 4 weeks). Two garlic cultivars, i.e. Sangga Sembalun and Lumbu Hijau were used as seed bulbs for OYDV elimination tests. Virus infection was confirmed using <em>transcription-polymerase chain reaction</em> (RT-PCR). The result showed that thermotherapy at 33 °Cwas the best method to eliminate OYDV in garlic although the efficiency was not the same for all cultivars. The efficiency reached up to 60% for cv. Lumbu Hijau, whereas for cv. Sangga Sembalun only reached up to 40%. Electrotherapy alone or in combination with thermotherapy were not able to produce OYDV-free plantlets.</p>http://journal.ipb.ac.id/index.php/jfiti/article/view/21882Lumbu Hijau, Sangga Sembalun, OYDV-free plantlets, RT-PCR |
| spellingShingle | Siti Shofiya Nasution Diny Dinarti Sri Hendrastuti Hidayat Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus Jurnal Fitopatologi Indonesia Lumbu Hijau, Sangga Sembalun, OYDV-free plantlets, RT-PCR |
| title | Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus |
| title_full | Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus |
| title_fullStr | Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus |
| title_full_unstemmed | Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus |
| title_short | Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus |
| title_sort | pengaruh elektroterapi dan termoterapi secara in vitro terhadap eliminasi onion yellow dwarf virus |
| topic | Lumbu Hijau, Sangga Sembalun, OYDV-free plantlets, RT-PCR |
| url | http://journal.ipb.ac.id/index.php/jfiti/article/view/21882 |
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