Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer
Homeobox A10 (Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of Hoxa10 in mouse endometrium can increase litter size. Moreover, Hoxa10 plays a key role in regulating the embryo implantation of sows. This study...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-05-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311919625827 |
| _version_ | 1819108150897278976 |
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| author | Qian XIAO Chang-zhi ZHAO Rui-yi LIN Guang-lei LI Chang-chun LI Hai-yan WANG Jing XU Sheng-song XIE Mei YU Shu-hong ZHAO |
| author_facet | Qian XIAO Chang-zhi ZHAO Rui-yi LIN Guang-lei LI Chang-chun LI Hai-yan WANG Jing XU Sheng-song XIE Mei YU Shu-hong ZHAO |
| author_sort | Qian XIAO |
| collection | DOAJ |
| description | Homeobox A10 (Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of Hoxa10 in mouse endometrium can increase litter size. Moreover, Hoxa10 plays a key role in regulating the embryo implantation of sows. This study aimed to generate transgenic pigs using Hoxa10 via somatic cell nuclear transfer (SCNT). We established seven Hoxa10-transgenic cell lines, and two of the cell lines were selected as nuclear donors for the transfer. A total of 1270 cloned embryos were generated and transferred to five surrogate mothers (Landrace×Yorkshire). Eight cloned male piglets were produced including one with cryptorchidism. Six transgenic piglets grew up healthy and produced 56 offspring. Finally, we obtained six transgenic male pigs and 26 transgenic positive offspring that can be used to further study the regulatory mechanism of Hoxa10 on the reproductive performance of pigs. |
| first_indexed | 2024-12-22T03:05:22Z |
| format | Article |
| id | doaj.art-80d5be1f709648f68ff8c44c00dbdade |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-22T03:05:22Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-80d5be1f709648f68ff8c44c00dbdade2022-12-21T18:41:04ZengElsevierJournal of Integrative Agriculture2095-31192019-05-0118510721079Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transferQian XIAO0Chang-zhi ZHAO1Rui-yi LIN2Guang-lei LI3Chang-chun LI4Hai-yan WANG5Jing XU6Sheng-song XIE7Mei YU8Shu-hong ZHAO9Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. China; College of Animal Science and Technology, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. China; College of Animal Science and Technology, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaKey Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, P.R. China; Correspondence ZHAO Shu-hongHomeobox A10 (Hoxa10) gene is one of the most important candidate genes associated with the reproductive performance of humans and mice. Overexpression of Hoxa10 in mouse endometrium can increase litter size. Moreover, Hoxa10 plays a key role in regulating the embryo implantation of sows. This study aimed to generate transgenic pigs using Hoxa10 via somatic cell nuclear transfer (SCNT). We established seven Hoxa10-transgenic cell lines, and two of the cell lines were selected as nuclear donors for the transfer. A total of 1270 cloned embryos were generated and transferred to five surrogate mothers (Landrace×Yorkshire). Eight cloned male piglets were produced including one with cryptorchidism. Six transgenic piglets grew up healthy and produced 56 offspring. Finally, we obtained six transgenic male pigs and 26 transgenic positive offspring that can be used to further study the regulatory mechanism of Hoxa10 on the reproductive performance of pigs.http://www.sciencedirect.com/science/article/pii/S2095311919625827Hoxa10 genetransgenic pigsomatic cell nuclear transferfetal fibroblastsoocyte |
| spellingShingle | Qian XIAO Chang-zhi ZHAO Rui-yi LIN Guang-lei LI Chang-chun LI Hai-yan WANG Jing XU Sheng-song XIE Mei YU Shu-hong ZHAO Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer Journal of Integrative Agriculture Hoxa10 gene transgenic pig somatic cell nuclear transfer fetal fibroblasts oocyte |
| title | Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer |
| title_full | Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer |
| title_fullStr | Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer |
| title_full_unstemmed | Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer |
| title_short | Production of homeobox A10 gene transgenic pigs by somatic cell nuclear transfer |
| title_sort | production of homeobox a10 gene transgenic pigs by somatic cell nuclear transfer |
| topic | Hoxa10 gene transgenic pig somatic cell nuclear transfer fetal fibroblasts oocyte |
| url | http://www.sciencedirect.com/science/article/pii/S2095311919625827 |
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