Effects of N-Terminus Substitution on the Structure and Spectroscopy of Gas-Phase Helices
Because of the importance of helices in the secondary structure of proteins, we have undertaken a study of their spectroscopy, fragmentation patterns and conformations in the gas phase. In this work, we describe the effects of substitution at the N-terminus of polyalanine helices capped wi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | deu |
| Published: |
Swiss Chemical Society
2008-04-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://chimia.ch/chimia/article/view/4470 |
| Summary: | Because of the importance of helices in the secondary structure of proteins, we have undertaken a study of their spectroscopy, fragmentation patterns and conformations in the gas phase. In this work, we describe the effects of substitution at the N-terminus of polyalanine helices capped
with a lysine at the C-terminus, namely Ac-Phe-(Ala)10-Lys-H+, Phe-(Ala)10-Lys-H+, and H+-Phe-(Ala)10-Lys-H+. Acetylation of the N-terminus has very little effect on the spectroscopy and structure, but protonation
of the N-terminus changes the infrared spectrum in such a way that we believe it may also change the structure of the peptide. The ultraviolet spectroscopy and fragmentation, on the other hand, are insensitive to either acetylation or protonation.
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| ISSN: | 0009-4293 2673-2424 |