Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10.
AIMS: Accumulating evidence suggest that numerous microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and differentiation, as well as various diseases that accompany inflammatory responses. Inflammation is known to be a major contributor to atherogenesis. Previous studies provi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3506618?pdf=render |
| _version_ | 1819117533100244992 |
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| author | Jianhua Zhu Ting Chen Lin Yang Zhoubin Li Mei Mei Wong Xiaoye Zheng Xiaoping Pan Li Zhang Hui Yan |
| author_facet | Jianhua Zhu Ting Chen Lin Yang Zhoubin Li Mei Mei Wong Xiaoye Zheng Xiaoping Pan Li Zhang Hui Yan |
| author_sort | Jianhua Zhu |
| collection | DOAJ |
| description | AIMS: Accumulating evidence suggest that numerous microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and differentiation, as well as various diseases that accompany inflammatory responses. Inflammation is known to be a major contributor to atherogenesis. Previous studies provide promising evidence in support of the role of miRNAs in cardiovascular disease. However, mechanistic data on these small molecules in atherosclerosis (AS) are still missing. The present study aims to investigate the potential role of miRNAs in AS. METHODS AND RESULTS: The miRNA transcriptase was verified by TaqMan real-time polymerase chain reaction assay. Thoracic aorta samples were obtained from Apolipoprotein E knockout mice, and plasma samples were from coronary artery disease (CAD) patients. The results showed that the miR-155 level was the most significantly elevated both in AS mice and CAD patients relative to the normal control. The functional role of miR-155 in the atherosclerotic path physiological process was also observed in vivo and in vitro. The observations suggested that miR-155 is a part of a negative feedback loop, which down-modulates inflammatory cytokine production and decreases AS progression. miR-155 was also found to mediate the inflammatory response and mitogen-activated protein kinase (MAPK) pathway by targeting mitogen-activated protein kinase kinase kinase 10. CONCLUSIONS: miR-155 contributes to the prevention of AS development and progression. It may also be involved in the posttranscriptional regulation of the inflammatory response and MAPK pathway by targeting mitogen-activated protein kinase kinase kinase 10. |
| first_indexed | 2024-12-22T05:34:29Z |
| format | Article |
| id | doaj.art-80da64aa5e6b446987d126cac731d655 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T05:34:29Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-80da64aa5e6b446987d126cac731d6552022-12-21T18:37:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4655110.1371/journal.pone.0046551Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10.Jianhua ZhuTing ChenLin YangZhoubin LiMei Mei WongXiaoye ZhengXiaoping PanLi ZhangHui YanAIMS: Accumulating evidence suggest that numerous microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and differentiation, as well as various diseases that accompany inflammatory responses. Inflammation is known to be a major contributor to atherogenesis. Previous studies provide promising evidence in support of the role of miRNAs in cardiovascular disease. However, mechanistic data on these small molecules in atherosclerosis (AS) are still missing. The present study aims to investigate the potential role of miRNAs in AS. METHODS AND RESULTS: The miRNA transcriptase was verified by TaqMan real-time polymerase chain reaction assay. Thoracic aorta samples were obtained from Apolipoprotein E knockout mice, and plasma samples were from coronary artery disease (CAD) patients. The results showed that the miR-155 level was the most significantly elevated both in AS mice and CAD patients relative to the normal control. The functional role of miR-155 in the atherosclerotic path physiological process was also observed in vivo and in vitro. The observations suggested that miR-155 is a part of a negative feedback loop, which down-modulates inflammatory cytokine production and decreases AS progression. miR-155 was also found to mediate the inflammatory response and mitogen-activated protein kinase (MAPK) pathway by targeting mitogen-activated protein kinase kinase kinase 10. CONCLUSIONS: miR-155 contributes to the prevention of AS development and progression. It may also be involved in the posttranscriptional regulation of the inflammatory response and MAPK pathway by targeting mitogen-activated protein kinase kinase kinase 10.http://europepmc.org/articles/PMC3506618?pdf=render |
| spellingShingle | Jianhua Zhu Ting Chen Lin Yang Zhoubin Li Mei Mei Wong Xiaoye Zheng Xiaoping Pan Li Zhang Hui Yan Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10. PLoS ONE |
| title | Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10. |
| title_full | Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10. |
| title_fullStr | Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10. |
| title_full_unstemmed | Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10. |
| title_short | Regulation of microRNA-155 in atherosclerotic inflammatory responses by targeting MAP3K10. |
| title_sort | regulation of microrna 155 in atherosclerotic inflammatory responses by targeting map3k10 |
| url | http://europepmc.org/articles/PMC3506618?pdf=render |
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