Effects of ouabain on vascular reactivity
Ouabain is an endogenous substance occurring in the plasma in the nanomolar range, that has been proposed to increase vascular resistance and induce hypertension. This substance acts on the<FONT FACE="Symbol"> a</font>-subunit of Na+,K+-ATPase inhibiting the Na+-pump activity....
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1997-04-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400016 |
| _version_ | 1811202566156976128 |
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| author | Vassallo D.V. E. Songu-Mize L.V. Rossoni S.M.C. Amaral |
| author_facet | Vassallo D.V. E. Songu-Mize L.V. Rossoni S.M.C. Amaral |
| author_sort | Vassallo D.V. |
| collection | DOAJ |
| description | Ouabain is an endogenous substance occurring in the plasma in the nanomolar range, that has been proposed to increase vascular resistance and induce hypertension. This substance acts on the<FONT FACE="Symbol"> a</font>-subunit of Na+,K+-ATPase inhibiting the Na+-pump activity. In the vascular smooth muscle this effect leads to intracellular Na+ accumulation that reduces the activity of the Na+/Ca2+ exchanger and to an increased vascular tone. It was also suggested that circulating ouabain, even in the nanomolar range, sensitizes the vascular smooth muscle to vasopressor substances. We tested the latter hypothesis by studying the effects of ouabain in the micromolar and nanomolar range on phenylephrine (PE)-evoked pressor responses. The experiments were performed in normotensive and hypertensive rats in vivo, under anesthesia, and in perfused rat tail vascular beds. The results showed that ouabain pretreatment increased the vasopressor responses to PE in vitro and in vivo. This sensitization after ouabain treatment was also observed in hypertensive animals which presented an enhanced vasopressor response to PE in comparison to normotensive animals. It is suggested that ouabain at nanomolar concentrations can sensitize vascular smooth muscle to vasopressor stimuli possibly contributing to increased tone in hypertension |
| first_indexed | 2024-04-12T02:42:15Z |
| format | Article |
| id | doaj.art-80da6fb1b101472b8dc6f6a12845aca3 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-12T02:42:15Z |
| publishDate | 1997-04-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-80da6fb1b101472b8dc6f6a12845aca32022-12-22T03:51:18ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1997-04-0130454510.1590/S0100-879X1997000400016Effects of ouabain on vascular reactivityVassallo D.V.E. Songu-MizeL.V. RossoniS.M.C. AmaralOuabain is an endogenous substance occurring in the plasma in the nanomolar range, that has been proposed to increase vascular resistance and induce hypertension. This substance acts on the<FONT FACE="Symbol"> a</font>-subunit of Na+,K+-ATPase inhibiting the Na+-pump activity. In the vascular smooth muscle this effect leads to intracellular Na+ accumulation that reduces the activity of the Na+/Ca2+ exchanger and to an increased vascular tone. It was also suggested that circulating ouabain, even in the nanomolar range, sensitizes the vascular smooth muscle to vasopressor substances. We tested the latter hypothesis by studying the effects of ouabain in the micromolar and nanomolar range on phenylephrine (PE)-evoked pressor responses. The experiments were performed in normotensive and hypertensive rats in vivo, under anesthesia, and in perfused rat tail vascular beds. The results showed that ouabain pretreatment increased the vasopressor responses to PE in vitro and in vivo. This sensitization after ouabain treatment was also observed in hypertensive animals which presented an enhanced vasopressor response to PE in comparison to normotensive animals. It is suggested that ouabain at nanomolar concentrations can sensitize vascular smooth muscle to vasopressor stimuli possibly contributing to increased tone in hypertensionhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400016ouabainNa+-pumphypertensionrat tail vascular bed perfusionarterial blood pressurevascular reactivityphenylephrine |
| spellingShingle | Vassallo D.V. E. Songu-Mize L.V. Rossoni S.M.C. Amaral Effects of ouabain on vascular reactivity Brazilian Journal of Medical and Biological Research ouabain Na+-pump hypertension rat tail vascular bed perfusion arterial blood pressure vascular reactivity phenylephrine |
| title | Effects of ouabain on vascular reactivity |
| title_full | Effects of ouabain on vascular reactivity |
| title_fullStr | Effects of ouabain on vascular reactivity |
| title_full_unstemmed | Effects of ouabain on vascular reactivity |
| title_short | Effects of ouabain on vascular reactivity |
| title_sort | effects of ouabain on vascular reactivity |
| topic | ouabain Na+-pump hypertension rat tail vascular bed perfusion arterial blood pressure vascular reactivity phenylephrine |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400016 |
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