Maximum tolerant dose and analgesic activity of PT1 peptide
Introduction: The article presents the results of the study of the maximum tolerant dose (MTD) and the analgesic activity of peptide PT1 isolated from Alopecosa marikovskyi spider venom. PT1 is the first compound of polypeptide nature, capable of exerting a selective modulating effect on purinergic...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Belgorod National Research University
2019-09-01
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| Series: | Research Results in Pharmacology |
| Online Access: | https://rrpharmacology.pensoft.net/article/38520/download/pdf/ |
| _version_ | 1797430473868181504 |
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| author | Yuliya A. Palikova Viktor A. Palikov Igor A. Dyachenko |
| author_facet | Yuliya A. Palikova Viktor A. Palikov Igor A. Dyachenko |
| author_sort | Yuliya A. Palikova |
| collection | DOAJ |
| description | Introduction: The article presents the results of the study of the maximum tolerant dose (MTD) and the analgesic activity of peptide PT1 isolated from Alopecosa marikovskyi spider venom. PT1 is the first compound of polypeptide nature, capable of exerting a selective modulating effect on purinergic P2X3 receptors.
Materials and methods: The study was conducted on 174 ICR mice. The analgesic activity of the peptide was evaluated in a thermal hypersensitivity test triggered by CFA and in a model of chemical irritation.
Results and discussion: The determined MTD for the peptide PT1 when administered intravenously provides evidence to attribute it to low-toxic compounds. The maximum analgesic activity of PT1 using the biomodel of hypersensitivity induced by CFA when tested 15 minutes after the administration was recorded at doses of 0.1 and 0.5 mg/kg. In the visceral pain test, the maximum analgesic activity 15 minutes after the administration of the chemical stimulus was observed at a dose of 0.01 mg/kg.
Conclusions: According to the results of testing peptide PT1, it is shown that it belongs to low-toxic compounds, has a pronounced analgesic activity in a wide range of doses of 0.0001–10 mg/kg. |
| first_indexed | 2024-03-09T09:29:04Z |
| format | Article |
| id | doaj.art-80dc4b118db64909a429911c2f3b83c8 |
| institution | Directory Open Access Journal |
| issn | 2658-381X |
| language | English |
| last_indexed | 2024-03-09T09:29:04Z |
| publishDate | 2019-09-01 |
| publisher | Belgorod National Research University |
| record_format | Article |
| series | Research Results in Pharmacology |
| spelling | doaj.art-80dc4b118db64909a429911c2f3b83c82023-12-02T05:04:14ZengBelgorod National Research UniversityResearch Results in Pharmacology2658-381X2019-09-0153374210.3897/rrpharmacology.5.3852038520Maximum tolerant dose and analgesic activity of PT1 peptideYuliya A. Palikova0Viktor A. Palikov1Igor A. Dyachenko2Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesBranch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesPushchino Scientific Center for Biological Research of the Russian Academy of SciencesIntroduction: The article presents the results of the study of the maximum tolerant dose (MTD) and the analgesic activity of peptide PT1 isolated from Alopecosa marikovskyi spider venom. PT1 is the first compound of polypeptide nature, capable of exerting a selective modulating effect on purinergic P2X3 receptors. Materials and methods: The study was conducted on 174 ICR mice. The analgesic activity of the peptide was evaluated in a thermal hypersensitivity test triggered by CFA and in a model of chemical irritation. Results and discussion: The determined MTD for the peptide PT1 when administered intravenously provides evidence to attribute it to low-toxic compounds. The maximum analgesic activity of PT1 using the biomodel of hypersensitivity induced by CFA when tested 15 minutes after the administration was recorded at doses of 0.1 and 0.5 mg/kg. In the visceral pain test, the maximum analgesic activity 15 minutes after the administration of the chemical stimulus was observed at a dose of 0.01 mg/kg. Conclusions: According to the results of testing peptide PT1, it is shown that it belongs to low-toxic compounds, has a pronounced analgesic activity in a wide range of doses of 0.0001–10 mg/kg.https://rrpharmacology.pensoft.net/article/38520/download/pdf/ |
| spellingShingle | Yuliya A. Palikova Viktor A. Palikov Igor A. Dyachenko Maximum tolerant dose and analgesic activity of PT1 peptide Research Results in Pharmacology |
| title | Maximum tolerant dose and analgesic activity of PT1 peptide |
| title_full | Maximum tolerant dose and analgesic activity of PT1 peptide |
| title_fullStr | Maximum tolerant dose and analgesic activity of PT1 peptide |
| title_full_unstemmed | Maximum tolerant dose and analgesic activity of PT1 peptide |
| title_short | Maximum tolerant dose and analgesic activity of PT1 peptide |
| title_sort | maximum tolerant dose and analgesic activity of pt1 peptide |
| url | https://rrpharmacology.pensoft.net/article/38520/download/pdf/ |
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