Survivability of freeze- and spray-dried probiotics and their effects on the growth and health performance of broilers
Background and Aim: Many strains of probiotics have been exploited and used as animal dietary supplements for broiler production. The efficacy and survival of probiotics during production may reflect better activities of the probiotics in the host. This study investigated the effects of freeze- and...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-09-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.16/September-2023/11.pdf |
| _version_ | 1797682534867271680 |
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| author | Juthamas Buahom Surasak Siripornadulsil Peerapol Sukon Treerat Sooksawat Wilailak Siripornadulsil |
| author_facet | Juthamas Buahom Surasak Siripornadulsil Peerapol Sukon Treerat Sooksawat Wilailak Siripornadulsil |
| author_sort | Juthamas Buahom |
| collection | DOAJ |
| description | Background and Aim: Many strains of probiotics have been exploited and used as animal dietary supplements for broiler production. The efficacy and survival of probiotics during production may reflect better activities of the probiotics in the host. This study investigated the effects of freeze- and spray-drying on the survivability and properties of probiotics and their ability to improve the growth and health performance of broilers.
Materials and Methods: Probiotic powders of four strains of lactic acid bacteria, Enterococcus faecium CA4, Enterococcus durans CH33, Ligilactobacillus salivarius CH24, Pediococcus acidilactici SH8, and Bacillus subtilis KKU213, were prepared using rice bran/chitosan/carboxy methyl cellulose as the carrier. The survival of each probiotic strain was investigated under stress conditions, including freeze-drying, spray-drying, and simulated gastrointestinal conditions. The body weight gain (BWG) and intestinal histomorphology were determined to assess broiler growth performance.
Results: All dried probiotics yielded a high survival rate during freeze-drying (95.8-98.6%) and spray-drying (94.4-98.2%). In addition, an analysis of the main effect revealed that the effectiveness of freeze-drying was higher than that of spray-drying in minimizing the loss of cell viability. The antimicrobial activity of all immobilized dried probiotic strains against Salmonella was maintained. The immobilized probiotics tolerated a low pH value of 2.0 and 0.5% (w/v) bile salt. Probiotic administration of a mixture of the five dried probiotics to 1-day-old hatched male broilers at early and late ages resulted in potential colonization in the broiler intestine, and enhancements in the BWG, lipid metabolism, and gut health (villus height and cryptal depth) were observed in the probiotic-treated groups.
Conclusion: The administration of three doses of the spray-dried probiotic mixture at days 15, 17, and 19 after hatching was sufficient to achieve long-term growth and health benefits in broilers. This finding might provide a cost-effective alternative to the administration of commonly used antibiotics in broiler production. |
| first_indexed | 2024-03-12T00:02:15Z |
| format | Article |
| id | doaj.art-80dd69887c354e38a9a5b83935075934 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-12T00:02:15Z |
| publishDate | 2023-09-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-80dd69887c354e38a9a5b839350759342023-09-17T15:21:08ZengVeterinary WorldVeterinary World0972-89882231-09162023-09-011691849186510.14202/vetworld.2023.1849-1865 Survivability of freeze- and spray-dried probiotics and their effects on the growth and health performance of broilersJuthamas Buahom0https://orcid.org/0000-0002-1356-8258Surasak Siripornadulsil1https://orcid.org/0000-0001-8396-4486Peerapol Sukon2https://orcid.org/0000-0002-0899-2572Treerat Sooksawat3https://orcid.org/0000-0002-8784-5617Wilailak Siripornadulsil4https://orcid.org/0000-0002-4911-7010Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand; Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand.Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand.Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand; Research Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen 40002, Thailand.Background and Aim: Many strains of probiotics have been exploited and used as animal dietary supplements for broiler production. The efficacy and survival of probiotics during production may reflect better activities of the probiotics in the host. This study investigated the effects of freeze- and spray-drying on the survivability and properties of probiotics and their ability to improve the growth and health performance of broilers. Materials and Methods: Probiotic powders of four strains of lactic acid bacteria, Enterococcus faecium CA4, Enterococcus durans CH33, Ligilactobacillus salivarius CH24, Pediococcus acidilactici SH8, and Bacillus subtilis KKU213, were prepared using rice bran/chitosan/carboxy methyl cellulose as the carrier. The survival of each probiotic strain was investigated under stress conditions, including freeze-drying, spray-drying, and simulated gastrointestinal conditions. The body weight gain (BWG) and intestinal histomorphology were determined to assess broiler growth performance. Results: All dried probiotics yielded a high survival rate during freeze-drying (95.8-98.6%) and spray-drying (94.4-98.2%). In addition, an analysis of the main effect revealed that the effectiveness of freeze-drying was higher than that of spray-drying in minimizing the loss of cell viability. The antimicrobial activity of all immobilized dried probiotic strains against Salmonella was maintained. The immobilized probiotics tolerated a low pH value of 2.0 and 0.5% (w/v) bile salt. Probiotic administration of a mixture of the five dried probiotics to 1-day-old hatched male broilers at early and late ages resulted in potential colonization in the broiler intestine, and enhancements in the BWG, lipid metabolism, and gut health (villus height and cryptal depth) were observed in the probiotic-treated groups. Conclusion: The administration of three doses of the spray-dried probiotic mixture at days 15, 17, and 19 after hatching was sufficient to achieve long-term growth and health benefits in broilers. This finding might provide a cost-effective alternative to the administration of commonly used antibiotics in broiler production.https://www.veterinaryworld.org/Vol.16/September-2023/11.pdfbroilersgrowth performanceimmobilized probioticsintestinal morphologylactic acid bacteria. |
| spellingShingle | Juthamas Buahom Surasak Siripornadulsil Peerapol Sukon Treerat Sooksawat Wilailak Siripornadulsil Survivability of freeze- and spray-dried probiotics and their effects on the growth and health performance of broilers Veterinary World broilers growth performance immobilized probiotics intestinal morphology lactic acid bacteria. |
| title | Survivability of freeze- and spray-dried probiotics and their effects on the growth and health performance of broilers |
| title_full | Survivability of freeze- and spray-dried probiotics and their effects on the growth and health performance of broilers |
| title_fullStr | Survivability of freeze- and spray-dried probiotics and their effects on the growth and health performance of broilers |
| title_full_unstemmed | Survivability of freeze- and spray-dried probiotics and their effects on the growth and health performance of broilers |
| title_short | Survivability of freeze- and spray-dried probiotics and their effects on the growth and health performance of broilers |
| title_sort | survivability of freeze and spray dried probiotics and their effects on the growth and health performance of broilers |
| topic | broilers growth performance immobilized probiotics intestinal morphology lactic acid bacteria. |
| url | https://www.veterinaryworld.org/Vol.16/September-2023/11.pdf |
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