Exosomes from MiR-126-Overexpressing Adscs Are Therapeutic in Relieving Acute Myocardial Ischaemic Injury
Background/Aims: Recent studies have indicated that exosomes play an important role in adipose-derived stem cell (ADSC) transplant-mediated ischaemic heart disease therapy. However, the treatment effect is not obvious. The aim of this study is to investigate whether ADSC-derived exosomes enriched wi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2017-12-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/485949 |
| _version_ | 1811267201220476928 |
|---|---|
| author | Qiancheng Luo Dongfeng Guo Guorong Liu Guo Chen Min Hang Mingming Jin |
| author_facet | Qiancheng Luo Dongfeng Guo Guorong Liu Guo Chen Min Hang Mingming Jin |
| author_sort | Qiancheng Luo |
| collection | DOAJ |
| description | Background/Aims: Recent studies have indicated that exosomes play an important role in adipose-derived stem cell (ADSC) transplant-mediated ischaemic heart disease therapy. However, the treatment effect is not obvious. The aim of this study is to investigate whether ADSC-derived exosomes enriched with microRNA (miR)-126 have a more protective effect on acute myocardial infarction (AMI). Methods: Exosomes were characterized by transmission electron microscopy, and the exosome particles were further examined using nanoparticle tracking analyses. A rat model of myocardial infarction and in vitro model of hypoxia-induced H9c2 myocardial cell injury were established to study the protective mechanism of exosomes from miR-126-overexpressing ADSCs. Results: The in vitro results showed that exosomes derived from miR-126-overexpressing ADSCs decreased H9c2 myocardial cell injury by reducing inflammation factor expression during hypoxia induction. The miR-126-enriched exosomes also decreased the expression of fibrosis-related proteins of H9c2 cells under hypoxic conditions. Matrigel® and Transwell® assays showed that miR-126-enriched exosomes significantly promoted microvascular generation and migration, respectively. In vivo studies confirmed that exosomes derived from ADSCs significantly decreased the myocardial injury area of infarction, especially after miR-126-enriched exosome treatment. Cardiac fibrosis and inflammatory cytokine expression were also decreased after treatment with miR-126-enriched exosomes. However, blood vessel formation was promoted in the infarction region of AMI rats. Conclusions: The results suggested that the expression of miR-126-enhanced ADSC-derived exosomes prevented myocardial damage by protecting myocardial cells from apoptosis, inflammation, fibrosis, and increased angiogenesis. |
| first_indexed | 2024-04-12T20:58:09Z |
| format | Article |
| id | doaj.art-80df215f32464b6cb3954b046de35318 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-04-12T20:58:09Z |
| publishDate | 2017-12-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-80df215f32464b6cb3954b046de353182022-12-22T03:16:55ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-12-014462105211610.1159/000485949485949Exosomes from MiR-126-Overexpressing Adscs Are Therapeutic in Relieving Acute Myocardial Ischaemic InjuryQiancheng LuoDongfeng GuoGuorong LiuGuo ChenMin HangMingming JinBackground/Aims: Recent studies have indicated that exosomes play an important role in adipose-derived stem cell (ADSC) transplant-mediated ischaemic heart disease therapy. However, the treatment effect is not obvious. The aim of this study is to investigate whether ADSC-derived exosomes enriched with microRNA (miR)-126 have a more protective effect on acute myocardial infarction (AMI). Methods: Exosomes were characterized by transmission electron microscopy, and the exosome particles were further examined using nanoparticle tracking analyses. A rat model of myocardial infarction and in vitro model of hypoxia-induced H9c2 myocardial cell injury were established to study the protective mechanism of exosomes from miR-126-overexpressing ADSCs. Results: The in vitro results showed that exosomes derived from miR-126-overexpressing ADSCs decreased H9c2 myocardial cell injury by reducing inflammation factor expression during hypoxia induction. The miR-126-enriched exosomes also decreased the expression of fibrosis-related proteins of H9c2 cells under hypoxic conditions. Matrigel® and Transwell® assays showed that miR-126-enriched exosomes significantly promoted microvascular generation and migration, respectively. In vivo studies confirmed that exosomes derived from ADSCs significantly decreased the myocardial injury area of infarction, especially after miR-126-enriched exosome treatment. Cardiac fibrosis and inflammatory cytokine expression were also decreased after treatment with miR-126-enriched exosomes. However, blood vessel formation was promoted in the infarction region of AMI rats. Conclusions: The results suggested that the expression of miR-126-enhanced ADSC-derived exosomes prevented myocardial damage by protecting myocardial cells from apoptosis, inflammation, fibrosis, and increased angiogenesis.https://www.karger.com/Article/FullText/485949ExosomesADSCsAcute myocardial ischaemiaMiR-126Apoptosis |
| spellingShingle | Qiancheng Luo Dongfeng Guo Guorong Liu Guo Chen Min Hang Mingming Jin Exosomes from MiR-126-Overexpressing Adscs Are Therapeutic in Relieving Acute Myocardial Ischaemic Injury Cellular Physiology and Biochemistry Exosomes ADSCs Acute myocardial ischaemia MiR-126 Apoptosis |
| title | Exosomes from MiR-126-Overexpressing Adscs Are Therapeutic in Relieving Acute Myocardial Ischaemic Injury |
| title_full | Exosomes from MiR-126-Overexpressing Adscs Are Therapeutic in Relieving Acute Myocardial Ischaemic Injury |
| title_fullStr | Exosomes from MiR-126-Overexpressing Adscs Are Therapeutic in Relieving Acute Myocardial Ischaemic Injury |
| title_full_unstemmed | Exosomes from MiR-126-Overexpressing Adscs Are Therapeutic in Relieving Acute Myocardial Ischaemic Injury |
| title_short | Exosomes from MiR-126-Overexpressing Adscs Are Therapeutic in Relieving Acute Myocardial Ischaemic Injury |
| title_sort | exosomes from mir 126 overexpressing adscs are therapeutic in relieving acute myocardial ischaemic injury |
| topic | Exosomes ADSCs Acute myocardial ischaemia MiR-126 Apoptosis |
| url | https://www.karger.com/Article/FullText/485949 |
| work_keys_str_mv | AT qianchengluo exosomesfrommir126overexpressingadscsaretherapeuticinrelievingacutemyocardialischaemicinjury AT dongfengguo exosomesfrommir126overexpressingadscsaretherapeuticinrelievingacutemyocardialischaemicinjury AT guorongliu exosomesfrommir126overexpressingadscsaretherapeuticinrelievingacutemyocardialischaemicinjury AT guochen exosomesfrommir126overexpressingadscsaretherapeuticinrelievingacutemyocardialischaemicinjury AT minhang exosomesfrommir126overexpressingadscsaretherapeuticinrelievingacutemyocardialischaemicinjury AT mingmingjin exosomesfrommir126overexpressingadscsaretherapeuticinrelievingacutemyocardialischaemicinjury |