Investigation of the impact of bacterial microencapsulation on natural product discovery
Developing new effective drugs to treat antibiotic resistant infections and cancer is urgently needed to reduce mortality due to these devastating diseases. Despite the promising potential of environmental microorganisms to produce such drugs, researchers are currently facing the problem of rediscov...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Current Research in Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590262823000394 |
| _version_ | 1797397555676446720 |
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| author | Tina Navaei Elias Madadian Bradley A. Haltli Christopher Cartmell Russell G. Kerr Ali Ahmadi |
| author_facet | Tina Navaei Elias Madadian Bradley A. Haltli Christopher Cartmell Russell G. Kerr Ali Ahmadi |
| author_sort | Tina Navaei |
| collection | DOAJ |
| description | Developing new effective drugs to treat antibiotic resistant infections and cancer is urgently needed to reduce mortality due to these devastating diseases. Despite the promising potential of environmental microorganisms to produce such drugs, researchers are currently facing the problem of rediscovering known compounds. To address this challenge, the impact of microencapsulation on natural compound production was explored using, Kitasatospora cystarginea NRRL B-16505 which is known to produce cystargamide and the 20S proteasome inhibitory cystargolide. The current work postulates that the stress of microencapsulation process may induce secondary metabolism, potentially leading to the production of novel metabolites. Different microencapsulation techniques, including microfluidics, co-axial air flow printing, and emulsification, were compared in terms of bead size, cell viability, metabolite profile, and metabolite yields. This study has shown promising results that may be leading to the discovery of new bioactive compounds as well as activating the silent pathways for compound production. Nutrition deficiency, reduced Oxygen, presence of salt, heat shock, bead uniformity, and shear stress during microencapsulation are potential reasons for the production of these putatively novel chemicals. |
| first_indexed | 2024-03-09T01:11:46Z |
| format | Article |
| id | doaj.art-80df6d7e24964a75bbdf1e23b0945f90 |
| institution | Directory Open Access Journal |
| issn | 2590-2628 |
| language | English |
| last_indexed | 2024-03-09T01:11:46Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Biotechnology |
| spelling | doaj.art-80df6d7e24964a75bbdf1e23b0945f902023-12-11T04:16:57ZengElsevierCurrent Research in Biotechnology2590-26282023-01-016100157Investigation of the impact of bacterial microencapsulation on natural product discoveryTina Navaei0Elias Madadian1Bradley A. Haltli2Christopher Cartmell3Russell G. Kerr4Ali Ahmadi5Faculty of Sustainable Design Engineering, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, CanadaDepartment of Mechanical Engineering, Ecole de technologie supérieure, University of Quebec, 1100, rue Notre-Dame Ouest, Montréal (Québec) H3C 1K3, Montreal, Quebec, CanadaNautilus Biosciences Croda, Regis and Joan Duffy Research Centre, 550 University Avenue, Charlottetown, PE C1A 4P3, CanadaDepartment of Pharmacology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA; Department of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, CanadaNautilus Biosciences Croda, Regis and Joan Duffy Research Centre, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada; Department of Chemistry, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada; Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada; Corresponding authors at: Nautilus Biosciences Croda, Regis and Joan Duffy Research Centre, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada (R.G. Kerr). Department of Mechanical Engineering, École de technologie supérieure, 1100, rue Notre-Dame Ouest, Montreal, QC H3C 1K3, Canada (A. Ahmadi).Faculty of Sustainable Design Engineering, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada; Department of Mechanical Engineering, Ecole de technologie supérieure, University of Quebec, 1100, rue Notre-Dame Ouest, Montréal (Québec) H3C 1K3, Montreal, Quebec, Canada; Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada; Corresponding authors at: Nautilus Biosciences Croda, Regis and Joan Duffy Research Centre, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada (R.G. Kerr). Department of Mechanical Engineering, École de technologie supérieure, 1100, rue Notre-Dame Ouest, Montreal, QC H3C 1K3, Canada (A. Ahmadi).Developing new effective drugs to treat antibiotic resistant infections and cancer is urgently needed to reduce mortality due to these devastating diseases. Despite the promising potential of environmental microorganisms to produce such drugs, researchers are currently facing the problem of rediscovering known compounds. To address this challenge, the impact of microencapsulation on natural compound production was explored using, Kitasatospora cystarginea NRRL B-16505 which is known to produce cystargamide and the 20S proteasome inhibitory cystargolide. The current work postulates that the stress of microencapsulation process may induce secondary metabolism, potentially leading to the production of novel metabolites. Different microencapsulation techniques, including microfluidics, co-axial air flow printing, and emulsification, were compared in terms of bead size, cell viability, metabolite profile, and metabolite yields. This study has shown promising results that may be leading to the discovery of new bioactive compounds as well as activating the silent pathways for compound production. Nutrition deficiency, reduced Oxygen, presence of salt, heat shock, bead uniformity, and shear stress during microencapsulation are potential reasons for the production of these putatively novel chemicals.http://www.sciencedirect.com/science/article/pii/S2590262823000394Natural productsMicroorganismsKitasatosporaMicroencapsulationMicrofluidics |
| spellingShingle | Tina Navaei Elias Madadian Bradley A. Haltli Christopher Cartmell Russell G. Kerr Ali Ahmadi Investigation of the impact of bacterial microencapsulation on natural product discovery Current Research in Biotechnology Natural products Microorganisms Kitasatospora Microencapsulation Microfluidics |
| title | Investigation of the impact of bacterial microencapsulation on natural product discovery |
| title_full | Investigation of the impact of bacterial microencapsulation on natural product discovery |
| title_fullStr | Investigation of the impact of bacterial microencapsulation on natural product discovery |
| title_full_unstemmed | Investigation of the impact of bacterial microencapsulation on natural product discovery |
| title_short | Investigation of the impact of bacterial microencapsulation on natural product discovery |
| title_sort | investigation of the impact of bacterial microencapsulation on natural product discovery |
| topic | Natural products Microorganisms Kitasatospora Microencapsulation Microfluidics |
| url | http://www.sciencedirect.com/science/article/pii/S2590262823000394 |
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