Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive
Olive crop is frequently treated with copper fungicides to combat foliar and fruit diseases such as olive leaf spot caused by <i>Fusicladium oleagineum</i> and anthracnose caused by <i>Colletotrichum</i> spp. The replacement of copper-based products with more eco-friendly alt...
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MDPI AG
2021-06-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/7/1667 |
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| author | Panagiota Ntasiou Alexandra Kaldeli Kerou Theodora Karamanidou Afrodite Vlachou George T. Tziros Alexander Tsouknidas George S. Karaoglanidis |
| author_facet | Panagiota Ntasiou Alexandra Kaldeli Kerou Theodora Karamanidou Afrodite Vlachou George T. Tziros Alexander Tsouknidas George S. Karaoglanidis |
| author_sort | Panagiota Ntasiou |
| collection | DOAJ |
| description | Olive crop is frequently treated with copper fungicides to combat foliar and fruit diseases such as olive leaf spot caused by <i>Fusicladium oleagineum</i> and anthracnose caused by <i>Colletotrichum</i> spp. The replacement of copper-based products with more eco-friendly alternatives is a priority. Metal nanoparticles synthesized in several ways have recently revolutionized crop protection with applications against important crop pathogens. In this study, we present the development of four copper-based nanoparticles (CuNP Type 1 to 4) synthesized with a wet chemistry approach. The CuNPs were characterized using Transmission Electron Microscopy, Dynamic Light Scattering, Laser Doppler Electrophoresis, and Attenuated Total Reflection measurements. In addition, the activity of the four CuNP types was tested in vitro and in planta against <i>F. oleagineum</i> and <i>Colletotrichum</i> spp. In vitro sensitivity measurements showed that for both pathogens, mycelial growth was the most susceptible developmental stage to the tested compounds. Against both pathogens, CuNP Type 1 and Type 2 were found to be more active in reducing mycelial growth compared to the reference commercial compounds of copper oxide and copper hydroxide. In planta experiments showed that CuNP Type 3 and CuNP Type 4 exhibited a strong protectant activity against both <i>F. oleagineum</i> and <i>Colletotrichum acutatum</i> with control efficacy values significantly higher than those achieved by the applications of either reference product. |
| first_indexed | 2024-03-10T10:04:41Z |
| format | Article |
| id | doaj.art-80e130e0869f4860b5dab1281b6e0616 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T10:04:41Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-80e130e0869f4860b5dab1281b6e06162023-11-22T01:38:55ZengMDPI AGNanomaterials2079-49912021-06-01117166710.3390/nano11071667Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of OlivePanagiota Ntasiou0Alexandra Kaldeli Kerou1Theodora Karamanidou2Afrodite Vlachou3George T. Tziros4Alexander Tsouknidas5George S. Karaoglanidis6Laboratory of Plant Pathology, Faculty of Agriculture, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, P.O. Box 269, 54124 Thessaloniki, GreecePLiN Nanotechnology S.A., Spectra Business Center 12th km Thessaloniki-Chalkidiki, Thermi, 57001 Thessaloniki, GreecePLiN Nanotechnology S.A., Spectra Business Center 12th km Thessaloniki-Chalkidiki, Thermi, 57001 Thessaloniki, GreecePLiN Nanotechnology S.A., Spectra Business Center 12th km Thessaloniki-Chalkidiki, Thermi, 57001 Thessaloniki, GreeceLaboratory of Plant Pathology, Faculty of Agriculture, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, P.O. Box 269, 54124 Thessaloniki, GreecePLiN Nanotechnology S.A., Spectra Business Center 12th km Thessaloniki-Chalkidiki, Thermi, 57001 Thessaloniki, GreeceLaboratory of Plant Pathology, Faculty of Agriculture, School of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, P.O. Box 269, 54124 Thessaloniki, GreeceOlive crop is frequently treated with copper fungicides to combat foliar and fruit diseases such as olive leaf spot caused by <i>Fusicladium oleagineum</i> and anthracnose caused by <i>Colletotrichum</i> spp. The replacement of copper-based products with more eco-friendly alternatives is a priority. Metal nanoparticles synthesized in several ways have recently revolutionized crop protection with applications against important crop pathogens. In this study, we present the development of four copper-based nanoparticles (CuNP Type 1 to 4) synthesized with a wet chemistry approach. The CuNPs were characterized using Transmission Electron Microscopy, Dynamic Light Scattering, Laser Doppler Electrophoresis, and Attenuated Total Reflection measurements. In addition, the activity of the four CuNP types was tested in vitro and in planta against <i>F. oleagineum</i> and <i>Colletotrichum</i> spp. In vitro sensitivity measurements showed that for both pathogens, mycelial growth was the most susceptible developmental stage to the tested compounds. Against both pathogens, CuNP Type 1 and Type 2 were found to be more active in reducing mycelial growth compared to the reference commercial compounds of copper oxide and copper hydroxide. In planta experiments showed that CuNP Type 3 and CuNP Type 4 exhibited a strong protectant activity against both <i>F. oleagineum</i> and <i>Colletotrichum acutatum</i> with control efficacy values significantly higher than those achieved by the applications of either reference product.https://www.mdpi.com/2079-4991/11/7/1667antifungal activitycopper nanoparticlesCuNPsnanofungicidesolive anthracnoseolive leaf spot |
| spellingShingle | Panagiota Ntasiou Alexandra Kaldeli Kerou Theodora Karamanidou Afrodite Vlachou George T. Tziros Alexander Tsouknidas George S. Karaoglanidis Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive Nanomaterials antifungal activity copper nanoparticles CuNPs nanofungicides olive anthracnose olive leaf spot |
| title | Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive |
| title_full | Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive |
| title_fullStr | Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive |
| title_full_unstemmed | Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive |
| title_short | Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive |
| title_sort | synthesis and characterization of novel copper nanoparticles for the control of leaf spot and anthracnose diseases of olive |
| topic | antifungal activity copper nanoparticles CuNPs nanofungicides olive anthracnose olive leaf spot |
| url | https://www.mdpi.com/2079-4991/11/7/1667 |
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