Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells
ABSTRACT Mesenchymal stem cell (MSC) therapy is a promising approach to curing bone diseases and disorders. In treating genetic bone disorders, MSC therapy is local or systemic transplantation of isolated and in vitro proliferated MSC rather than bone marrow transplantation. Recent evidence showed t...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | JBMR Plus |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jbm4.10722 |
| _version_ | 1797868081443241984 |
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| author | Hotaka Kawai May Wathone Oo Kiyofumi Takabatake Ikue Tosa Yamin Soe Htoo Shwe Eain Sho Sanou Shigeko Fushimi Shintaro Sukegawa Keisuke Nakano Takarada Takeshi Hitoshi Nagatsuka |
| author_facet | Hotaka Kawai May Wathone Oo Kiyofumi Takabatake Ikue Tosa Yamin Soe Htoo Shwe Eain Sho Sanou Shigeko Fushimi Shintaro Sukegawa Keisuke Nakano Takarada Takeshi Hitoshi Nagatsuka |
| author_sort | Hotaka Kawai |
| collection | DOAJ |
| description | ABSTRACT Mesenchymal stem cell (MSC) therapy is a promising approach to curing bone diseases and disorders. In treating genetic bone disorders, MSC therapy is local or systemic transplantation of isolated and in vitro proliferated MSC rather than bone marrow transplantation. Recent evidence showed that bone marrow MSC engraftment to bone regeneration has been controversial in animal and human studies. Here, our modified bone marrow transplantation (BMT) method solved this problem. Like routine BMT, our modified method involves three steps: (i) isolation of bone marrow cells from the donor, (ii) whole‐body lethal irradiation to the recipient, and (iii) injection of isolated bone marrow cells into irradiated recipient mice via the tail vein. The significant modification is imported at the bone marrow isolation step. While the bone marrow cells are flushed out from the bone marrow with the medium in routine BMT, we applied the enzymes’ (collagenase type 4 and dispase) integrated medium to wash out the bone marrow cells. Then, cells were incubated in enzyme integrated solution at 37°C for 10 minutes. This modification designated BMT as collagenase‐integrated BMT (c‐BMT). Notably, successful engraftment of bone marrow MSC to the new bone formation, such as osteoblasts and chondrocytes, occurs in c‐BMT mice, whereas routine BMT mice do not recruit bone marrow MSC. Indeed, flow cytometry data showed that c‐BMT includes a higher proportion of LepR+, CD51+, or RUNX2+ non‐hematopoietic cells than BMT. These findings suggested that c‐BMT is a time‐efficient and more reliable technique that ensures the disaggregation and collection of bone marrow stem cells and engraftment of bone marrow MSC to the recipient. Hence, we proposed that c‐BMT might be a promising approach to curing genetic bone disorders. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research. |
| first_indexed | 2024-04-09T23:51:31Z |
| format | Article |
| id | doaj.art-80e2ce5e69d548a5ae6ce9ba9e0aff5b |
| institution | Directory Open Access Journal |
| issn | 2473-4039 |
| language | English |
| last_indexed | 2024-04-09T23:51:31Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | JBMR Plus |
| spelling | doaj.art-80e2ce5e69d548a5ae6ce9ba9e0aff5b2023-03-17T08:30:27ZengWileyJBMR Plus2473-40392023-03-0173n/an/a10.1002/jbm4.10722Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem CellsHotaka Kawai0May Wathone Oo1Kiyofumi Takabatake2Ikue Tosa3Yamin Soe4Htoo Shwe Eain5Sho Sanou6Shigeko Fushimi7Shintaro Sukegawa8Keisuke Nakano9Takarada Takeshi10Hitoshi Nagatsuka11Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Regenerative Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Regenerative Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama JapanABSTRACT Mesenchymal stem cell (MSC) therapy is a promising approach to curing bone diseases and disorders. In treating genetic bone disorders, MSC therapy is local or systemic transplantation of isolated and in vitro proliferated MSC rather than bone marrow transplantation. Recent evidence showed that bone marrow MSC engraftment to bone regeneration has been controversial in animal and human studies. Here, our modified bone marrow transplantation (BMT) method solved this problem. Like routine BMT, our modified method involves three steps: (i) isolation of bone marrow cells from the donor, (ii) whole‐body lethal irradiation to the recipient, and (iii) injection of isolated bone marrow cells into irradiated recipient mice via the tail vein. The significant modification is imported at the bone marrow isolation step. While the bone marrow cells are flushed out from the bone marrow with the medium in routine BMT, we applied the enzymes’ (collagenase type 4 and dispase) integrated medium to wash out the bone marrow cells. Then, cells were incubated in enzyme integrated solution at 37°C for 10 minutes. This modification designated BMT as collagenase‐integrated BMT (c‐BMT). Notably, successful engraftment of bone marrow MSC to the new bone formation, such as osteoblasts and chondrocytes, occurs in c‐BMT mice, whereas routine BMT mice do not recruit bone marrow MSC. Indeed, flow cytometry data showed that c‐BMT includes a higher proportion of LepR+, CD51+, or RUNX2+ non‐hematopoietic cells than BMT. These findings suggested that c‐BMT is a time‐efficient and more reliable technique that ensures the disaggregation and collection of bone marrow stem cells and engraftment of bone marrow MSC to the recipient. Hence, we proposed that c‐BMT might be a promising approach to curing genetic bone disorders. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.https://doi.org/10.1002/jbm4.10722BONE FORMATIONBONE MARROW MESENCHYMAL STEM CELLSBONE MARROW TRANSPLANTATION MODELOSTEOBLASTSSYSTEM BIOLOGY—BONE INTERACTOR |
| spellingShingle | Hotaka Kawai May Wathone Oo Kiyofumi Takabatake Ikue Tosa Yamin Soe Htoo Shwe Eain Sho Sanou Shigeko Fushimi Shintaro Sukegawa Keisuke Nakano Takarada Takeshi Hitoshi Nagatsuka Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells JBMR Plus BONE FORMATION BONE MARROW MESENCHYMAL STEM CELLS BONE MARROW TRANSPLANTATION MODEL OSTEOBLASTS SYSTEM BIOLOGY—BONE INTERACTOR |
| title | Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells |
| title_full | Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells |
| title_fullStr | Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells |
| title_full_unstemmed | Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells |
| title_short | Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells |
| title_sort | enzyme cleaved bone marrow transplantation improves the engraftment of bone marrow mesenchymal stem cells |
| topic | BONE FORMATION BONE MARROW MESENCHYMAL STEM CELLS BONE MARROW TRANSPLANTATION MODEL OSTEOBLASTS SYSTEM BIOLOGY—BONE INTERACTOR |
| url | https://doi.org/10.1002/jbm4.10722 |
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