Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening
Watermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening. To date, the molecular mechanics of watermelon ripening remain unclear. Genetic and transcriptome evidences suggested that the ethylene response factor (ERF) gene ClERF069 may be an important candidat...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2020-07-01
|
| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014120300558 |
| _version_ | 1797203994641170432 |
|---|---|
| author | Ming Zhou Shaogui Guo Shouwei Tian Jie Zhang Yi Ren Guoyi Gong Changbao Li Haiying Zhang Yong Xu |
| author_facet | Ming Zhou Shaogui Guo Shouwei Tian Jie Zhang Yi Ren Guoyi Gong Changbao Li Haiying Zhang Yong Xu |
| author_sort | Ming Zhou |
| collection | DOAJ |
| description | Watermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening. To date, the molecular mechanics of watermelon ripening remain unclear. Genetic and transcriptome evidences suggested that the ethylene response factor (ERF) gene ClERF069 may be an important candidate factor affecting watermelon fruit ripening. To dissect the roles of ClERF069 in fruit ripening, structure and phylogenetic analysis were performed using the amplified full-length sequence. Normal-ripening watermelon 97103, non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening indexs. The results indicated that ClERF069 belongs to ERF family group VI and show high homology (83% identity) to melon ERF069-like protein. ClERF069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening progress. Further transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 days. Lycopene, β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit ripening. Taken together, watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening. |
| first_indexed | 2024-04-24T08:28:11Z |
| format | Article |
| id | doaj.art-80e301c8331e44558a0bd830023476b9 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:28:11Z |
| publishDate | 2020-07-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-80e301c8331e44558a0bd830023476b92024-04-16T20:57:31ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412020-07-0164247256Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit RipeningMing Zhou0Shaogui Guo1Shouwei Tian2Jie Zhang3Yi Ren4Guoyi Gong5Changbao Li6Haiying Zhang7Yong Xu8Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaCorresponding author. Tel: 86 10 51503199; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaWatermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening. To date, the molecular mechanics of watermelon ripening remain unclear. Genetic and transcriptome evidences suggested that the ethylene response factor (ERF) gene ClERF069 may be an important candidate factor affecting watermelon fruit ripening. To dissect the roles of ClERF069 in fruit ripening, structure and phylogenetic analysis were performed using the amplified full-length sequence. Normal-ripening watermelon 97103, non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening indexs. The results indicated that ClERF069 belongs to ERF family group VI and show high homology (83% identity) to melon ERF069-like protein. ClERF069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening progress. Further transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 days. Lycopene, β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit ripening. Taken together, watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening.http://www.sciencedirect.com/science/article/pii/S2468014120300558watermelonethylene response factorERFtransgenic tomatoripening |
| spellingShingle | Ming Zhou Shaogui Guo Shouwei Tian Jie Zhang Yi Ren Guoyi Gong Changbao Li Haiying Zhang Yong Xu Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening Horticultural Plant Journal watermelon ethylene response factor ERF transgenic tomato ripening |
| title | Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening |
| title_full | Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening |
| title_fullStr | Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening |
| title_full_unstemmed | Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening |
| title_short | Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening |
| title_sort | overexpression of the watermelon ethylene response factor clerf069 in transgenic tomato resulted in delayed fruit ripening |
| topic | watermelon ethylene response factor ERF transgenic tomato ripening |
| url | http://www.sciencedirect.com/science/article/pii/S2468014120300558 |
| work_keys_str_mv | AT mingzhou overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening AT shaoguiguo overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening AT shouweitian overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening AT jiezhang overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening AT yiren overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening AT guoyigong overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening AT changbaoli overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening AT haiyingzhang overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening AT yongxu overexpressionofthewatermelonethyleneresponsefactorclerf069intransgenictomatoresultedindelayedfruitripening |